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2 protocols using fenofibrate

1

Quantitative Bioanalytical Workflow

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LC-MS grade acetonitrile, methanol, and water were purchased from J.T. Baker (Milwaukee, WI, USA). Amlodipine, atenolol, atorvastatin, digoxin, enalapril, losartan, propranolol, simvastatin, and sulfameter were purchased from Sigma–Aldrich (St. Louis, MO, USA). Fenofibrate, furosemide, nifedipine, and valsartan were purchased from Tokyo Chemical Industry (Tokyo, Japan). Whatman 903 blood sampling cards were purchased from GE Healthcare (Westborough, MA, USA). Human whole blood with EDTA was purchased from Innovative Research (Novi, MI, USA), which is a supplier of human blood samples. Experimental usage of human blood was approved by the Institutional Review Board of Seoul National University (IRB NO. E1605/003-003).
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2

Preparation of Chemical Stock Solutions

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Fenofibrate and gemfibrozil were obtained from Tokyo Chemical Industry (Tokyo, Japan). Methimazole, propylthiouracil, thyroxine, and fatostatin were obtained from Sigma (St. Louis, MO, USA). Stock solutions of these chemicals were prepared by dissolving in dimethyl sulfoxide (Nacalai Tesque, Kyoto, Japan). 2-Phenoxyethanol was obtained from Wako Chemical (Osaka, Japan).
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