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Enzyme linked immunosorbent assay kit for porcine il 6

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The Enzyme-linked immunosorbent assay (ELISA) kit for porcine IL-6 is a laboratory tool used to quantify the levels of interleukin-6 (IL-6) in porcine biological samples. It utilizes the ELISA technique to detect and measure the concentration of the target analyte.

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Porcine IL-6 IL-6 assay kit IL-6 kits

2 protocols using enzyme linked immunosorbent assay kit for porcine il 6

1

Quantifying Porcine IL-6 in TGEV Infection

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The amount of porcine IL-6 cytokine was measured as described (Yang et al., 2017b (link)). Briefly, the supernatant of TGEV infected ST cells at an MOI of 0.01 was harvested at the indicated periods and the amount of secreted IL-6 was determined using an enzyme-linked immunosorbent assay kit for porcine IL-6 (R&D Systems, Inc.). Appropriate dilutions were performed prior to determination of the amounts of IL-6 when the harvested supernatants contained concentrations of IL-6 too high to be accurately assayed using the enzyme-linked immunosorbent assay kit.
Yang C.W., Chang H.Y., Lee Y.Z., Hsu H.Y, & Lee S.J. (2018). The cardenolide ouabain suppresses coronaviral replication via augmenting a Na+/K+-ATPase-dependent PI3K_PDK1 axis signaling. Toxicology and Applied Pharmacology, 356, 90-97.
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2

Quantifying TGEV-Induced IL-6 Secretion

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The supernatant of TGEV infected ST cells (MOI of 7) with an indicated treatment at 6 h.p.i. was harvested and diluted to proper concentrations to determine the amount of IL-6 secreted using enzyme-linked immunosorbent assay kit for porcine IL-6 (R&D Systems, Inc.), per the manufacturer’s recommendations.
Yang C.W., Lee Y.Z., Hsu H.Y., Shih C., Chao Y.S., Chang H.Y, & Lee S.J. (2017). Targeting Coronaviral Replication and Cellular JAK2 Mediated Dominant NF-κB Activation for Comprehensive and Ultimate Inhibition of Coronaviral Activity. Scientific Reports, 7, 4105.
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