May grunwald solution

Manufactured by Merck Group

Sourced in Germany, United States

May-Grunwald solution is a biological stain used in microscopy for the differentiation of blood cells. It is a mixture of methylene blue and eosin dyes that stains cellular components, allowing for the identification and analysis of various blood cell types.

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Lab products found in correlation

Giemsa solution, by Merck Group (5 mentions) Cytospin, by Hanil (2 mentions) Shandon cytospin 4, by Thermo Fisher Scientific (1 mentions) Dm2000 inverted microscope, by Leica (1 mentions) Giemsa stain, by Merck Group (1 mentions) Anthrone reagent, by Merck Group (1 mentions) Chloroquine diphosphate, by Merck Group (1 mentions) Dimethyl sulphoxide, by Merck Group (1 mentions) Sigmacote, by Merck Group (1 mentions) Sulphuric acid h2so4, by Avantor (1 mentions) Application suite, by Leica (1 mentions) Vectamount, by Vector Laboratories (1 mentions)

Close spelling variants of this product

May-Grunwald (15 citations) May-Grunwald-Giemsa solution (3 citations) May-Grunwald and Giemsa staining solution (2 citations) May-Grunwald solution 1 (1 citations) May-Grunwald’s solution (1 citations)

6 protocols using may grunwald solution

Analyzing BALF Cell Composition in BALB/c Mice

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After anesthetizing the BALB/c mice with alfaxan (Alfaxalone^®, Jurox Pty Ltd., Hunter Valley, Australia), the mouse lungs were lavaged three times with cold 1× PBS (yield: 80%, total volume = 0.8 mL). After centrifugation of BALF at 2,000× g for 5 min at 4 °C, the supernatant was collected for ELISA analysis and the pellets were used for cell analysis. Total cells of the BALF pellet were attached to a slide glass using a cytospin (5 min, 500 rpm, Hanil Electric, Wonju, Korea), then fixed in methanol for 30 s. These slides were subsequently processed in May–Grunwald solution (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) for 5 min, then in Giemsa solution (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) for 12 min. After rinsing three times, the slides were covered, after which immune cells were counted under light microscopy at 400× magnification.

Choi J.Y., Kim J.E., Park J.J., Lee M.R., Song B.R., Park J.W., Kang M.J., Lee H.S., Son H.J., Hong J.T, & Hwang D.Y. (2018). The Anti-Inflammatory Effects of Fermented Herbal Roots of Asparagus cochinchinensis in an Ovalbumin-Induced Asthma Model. Journal of Clinical Medicine, 7(10), 377.

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Cytospin Cell Staining Protocol

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10⁵ cells were cytospun for 5 min at 300 × g onto glass slides. Slides were then stained for 3 min with May–Grunwald solution (Sigma-Aldrich) at room temperature. After washing in water, they were incubated for 20 min in Giemsa solution (Sigma-Aldrich) (1:20 in water). Slides were washed again in water before being mounted with Mowiol embedding medium.

Tzelepis K., De Braekeleer E., Aspris D., Barbieri I., Vijayabaskar M.S., Liu W.H., Gozdecka M., Metzakopian E., Toop H.D., Dudek M., Robson S.C., Hermida-Prado F., Yang Y.H., Babaei-Jadidi R., Garyfallos D.A., Ponstingl H., Dias J.M., Gallipoli P., Seiler M., Buonamici S., Vick B., Bannister A.J., Rad R., Prinjha R.K., Marioni J.C., Huntly B., Batson J., Morris J.C., Pina C., Bradley A., Jeremias I., Bates D.O., Yusa K., Kouzarides T, & Vassiliou G.S. (2018). SRPK1 maintains acute myeloid leukemia through effects on isoform usage of epigenetic regulators including BRD4. Nature Communications, 9, 5378.

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Cytospin Cell Staining Protocol

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Cytospins were prepared on glass slides (5×10⁴ cells in 200 μL of PBS), using the Thermo Scientific Shandon 4 Cytospin (300 g for 5 min). Slides were stained with May-Grunwald solution (Sigma-Aldrich) for 5 min, rinsed in 40 mM Tris buffer for 90 s, and subsequently stained with Giemsa solution (Sigma-Aldrich) for 15 minutes. Cells were imaged using a Leica DM2000 inverted microscope under 100 × objective magnification.

Gonzalez-Menendez P., Romano M., Yan H., Deshmukh R., Papoin J., Oburoglu L., Daumur M., Dumé A.S., Phadke I., Mongellaz C., Qu X., Bories P.N., Fontenay M., An X., Dardalhon V., Sitbon M., Zimmermann V.S., Gallagher P.G., Tardito S., Blanc L., Mohandas N., Taylor N, & Kinet S. (2021). An IDH1-vitamin C crosstalk drives human erythroid development by inhibiting pro-oxidant mitochondrial metabolism. Cell reports, 34(5), 108723.

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Phytochemical Analysis of Clove Extract

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All drugs and chemicals which were of analytical grade quality were sourced from standard pharmaceutical suppliers. Chloroquine diphosphate (C₁₈H₂₆CIN₃∙2H₃PO₄), anthrone reagent, sigmacote, dimethyl sulphoxide (DMSO), Giemsa stain and May-Grunwald solution (Sigma-Aldrich Chemical Company, St Louis, Missouri, USA); calcium chloride (CaCl₂), potassium hydroxide (KOH), sodium sulphate (Na₂SO₄), sodium hydroxide (NaOH), potassium dihydrogen phosphate (KH₂PO₄) and 95% ethanol (C₂H₅OH) (Merck Chemicals (PTY) LTD, Johannesburg, South Africa); diethyl ether (C₄H₁₀O) (NT Laboratory Supplies (PTY) LTD, Johannesburg, South Africa); sulphuric acid (H₂SO₄) (BDH Chemicals LTD, Poole, Dorset, England), halothane (Fluorothane^®, AstraZeneca Pharmaceuticals (PTY) LTD, Johannesburg, South Africa) and insulin ELISA kit (Mecrodia AB, Uppsala, Sweden). S. aromaticum dried flower buds were purchased from Kies Supermarket (Reservoir Hills, Durban, South Africa)

Sibiya H.P., Mabandla M.V, & Musabayane C.T. (2016). The Effects of Transdermally Delivered Oleanolic Acid on Malaria Parasites and Blood Glucose Homeostasis in P. berghei-Infected Male Sprague-Dawley Rats. PLoS ONE, 11(12), e0167132.

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BALF Cell Analysis in BALB/c Mice

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After the BALB/c mice (n = 4) were anaesthetized with alfaxan (Alfaxalone®, Jurox Pty Ltd., Hunter Valley, Australia), BALF was obtained (yield: 80%, total volume of 0.8 ml) from mice of subset groups via tracheal cannulation using cold 1× PBS. After the centrifugation of BALF at 2000 x g for 5 min at 4°C, the supernatant were collected for ELISA analysis and the pellets were used for cell analysis. Total cells of BALF pellet were attached to slide glass using a cytospin (5 min, 500 x g, Hanil Electric, Wonju, Korea) and fixed in methanol for 30 s. These slides were processed in May-Grunwald solution (Sigma-Aldrich Co.) for 5 min, and subsequently in Giemsa solution (Sigma-Aldrich Co.) for 12 min. After rinsing with three times, the slides were covered, and then, immune cells were counted using the Leica Application Suite (Leica Microsystems, Wetzlar, Germany) at 400× magnification.

Choi J.Y., Kim S.H., Kim J.E., Park J.W., Kang M.J., Choi H.J., Bae S.J., Lee J.H., Jung Y.S, & Hwang D.Y. (2019). Four amino acids as serum biomarkers for anti-asthma effects in the ovalbumin-induced asthma mouse model treated with extract of Asparagus cochinchinensis. Laboratory Animal Research, 35, 32.

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Erythroid Morphology Analysis Protocol

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5 × 10⁴ to 1 × 10⁵ were collected onto microscope slides with a cytocentrifuge (Shandon/Thermo Scientific) and air dried. Slides were stained in May-Grunwald solution (Sigma) and then stained in 1:33 dilution of Giemsa solution (Sigma) for 12 minutes. Slides were washed twice in water and air dried prior to Vectamount (Vector Laboratories) and coverslip. Erythroid morphology was quantified as basophilic, polychromatic, orthochromatic erythroblasts, or reticulocytes.

Clough C.A., Pangallo J., Sarchi M., Ilagan J.O., North K., Bergantinos R., Stolla M.C., Naru J., Nugent P., Kim E., Stirewalt D.L., Subramaniam A.R., Abdel-Wahab O., Abkowitz J.L., Bradley R.K, & Doulatov S. (2022). Coordinated missplicing of TMEM14C and ABCB7 causes ring sideroblast formation in SF3B1-mutant myelodysplastic syndrome. Blood, 139(13), 2038-2049.

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