Surface staining was carried out using PBS with 1% FBS. For intracellular staining of cytokines, cells were first surface stained, then fixed with 1% paraformaldehyde (catalog no. 19943; Affymetrix, Santa Clara, CA) for 15 min at room temperature and permeabilized in permeabilization buffer (TNB-1213-L150; Tonbo Biosciences) before staining for cytokines. Staining for transcription factors was performed with the Foxp3/Transcription Factor Staining Buffer Kit (TNB-1022-L160; Tonbo Biosciences) according to the manufacturer’s instructions. Flow cytometry data were collected with CytoFLEX (BD Biosciences, Franklin Lake, NJ), and results were analyzed using FlowJo software (Becton Dickinson, Franklin Lakes, NJ). Abs specific for mouse CD4 (RM4–5), CD8 (53–6.7), and IL-4 (11B11) were purchased from Tonbo Biosciences; and those for IFN-γ (4S.B3), TNF (MAB11), GATA3 (L50–823), and Tbet (eBio4B10) were purchased from eBioscience (San Diego, CA). Abs specific for human CD4 (RPA-T4), CD8 (RPA-T8), and IL-4 (MP4–25D2) were purchased from Tonbo Biosciences; and those for IFN-γ (4S.B3), GATA3 (16E10A23), and Tbet (eBio4B10) were obtained from eBioscience.
Mp4 25d2
Manufactured by Cytek Biosciences
The MP4-25D2 is a multi-parameter flow cytometer designed for high-resolution analysis of complex biological samples. It features four lasers and up to 25 detection channels, enabling comprehensive characterization of cell populations. The core function of the MP4-25D2 is to provide researchers with a powerful tool for advanced multicolor flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Paraformaldehyde (pfa), by Thermo Fisher Scientific (2 mentions)
Foxp3 transcription factor staining buffer kit, by Cytek Biosciences (2 mentions)
Tnb 1213 l150, by Cytek Biosciences (2 mentions)
Rm4 5, by Cytek Biosciences (2 mentions)
Ebio4b10, by Thermo Fisher Scientific (2 mentions)
Rpa t4, by Cytek Biosciences (2 mentions)
Rpa t8, by Cytek Biosciences (2 mentions)
2 protocols using mp4 25d2
1
Comprehensive Cytokine Profiling with Flow Cytometry
2
Comprehensive Cytokine Profiling with Flow Cytometry
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Surface staining was carried out using PBS with 1% FBS. For intracellular staining of cytokines, cells were first surface stained, then fixed with 1% paraformaldehyde (catalog no. 19943; Affymetrix, Santa Clara, CA) for 15 min at room temperature and permeabilized in permeabilization buffer (TNB-1213-L150; Tonbo Biosciences) before staining for cytokines. Staining for transcription factors was performed with the Foxp3/Transcription Factor Staining Buffer Kit (TNB-1022-L160; Tonbo Biosciences) according to the manufacturer’s instructions. Flow cytometry data were collected with CytoFLEX (BD Biosciences, Franklin Lake, NJ), and results were analyzed using FlowJo software (Becton Dickinson, Franklin Lakes, NJ). Abs specific for mouse CD4 (RM4–5), CD8 (53–6.7), and IL-4 (11B11) were purchased from Tonbo Biosciences; and those for IFN-γ (4S.B3), TNF (MAB11), GATA3 (L50–823), and Tbet (eBio4B10) were purchased from eBioscience (San Diego, CA). Abs specific for human CD4 (RPA-T4), CD8 (RPA-T8), and IL-4 (MP4–25D2) were purchased from Tonbo Biosciences; and those for IFN-γ (4S.B3), GATA3 (16E10A23), and Tbet (eBio4B10) were obtained from eBioscience.
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