Single-cell suspensions with 1×105 cells/mL in concentration in PBS (HyClone) were prepared. They were loaded onto microfluidic devices and scRNA-seq libraries were constructed according to the manufacturer’s (Singleron GEXSCOPE®) protocol using the GEXSCOPE® Single-Cell RNA Library Kit (Singleron Biotechnologies) (20 (link)). Individual libraries were then diluted to 4nM and pooled for sequencing. These were sequenced on Illumina HiSeq X with 150 bp paired-end reads. The scRNA-Seq data are available at the Gene Expression Omnibus (https://www.ncbi.nlm.nih.gov/geo/ . GEO accession number: GSE190329).
Gexscope
Manufactured by Singleron Biotechnologies
Sourced in China
The GEXSCOPE is a high-performance single-cell multi-omics analysis platform developed by Singleron Biotechnologies. It enables simultaneous profiling of gene expression, DNA methylation, and chromatin accessibility at the single-cell level.
Automatically generated - may contain errors
Lab products found in correlation
Gexscope single cell rna library kit, by Singleron Biotechnologies (10 mentions)
Hiseq x, by Illumina (6 mentions)
Thermal cycler, by Bio-Rad (2 mentions)
Novaseq 6000, by Illumina (2 mentions)
Fragment analyzer, by Illumina (1 mentions)
Novaseq platform, by Illumina (1 mentions)
Gexscope tissue preservation solution, by Singleron Biotechnologies (1 mentions)
Singleron matrix automated single cell processing system, by Singleron Biotechnologies (1 mentions)
Hyclone, by Cytiva (1 mentions)
C57bl 6j mice, by Charles River Laboratories (1 mentions)
Scellive tissue preservation solution, by Singleron Biotechnologies (1 mentions)
Novaseq, by Illumina (1 mentions)
Hiseq x platform, by Illumina (1 mentions)
13 protocols using gexscope
1
Single-Cell RNA-seq with GEXSCOPE® Kit
2
Single-Cell RNA Sequencing Workflow
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The prepared single-cell suspension in PBS was loaded onto a single-cell microfluidic chip according to the manufacturer’s protocols (Singleron GEXSCOPE). Then, 60 μl of resuspended barcoded beads was slowly injected onto the chip over 30 s to allow the beads to slowly spread inside the chip. After slowly adding 100 μl of lysis buffer mix to the chip, the cells were lysed, and mRNA was released during incubation at room temperature for 20 min. Excess liquid from the inlet and outlet reservoirs was immediately removed. Emulsions were immediately acquired from the chip and incubated in a thermal cycler (Bio-Rad) for reverse transcription. The scRNA-seq library preparation was constructed according to the manufacturer’s protocol. The captured mRNA was incubated in a preheated ThermoMixer with the reverse transcription mix at 42°C at 1000 rpm for 90 min. Using PCR to amplify 10 or 50 ng of barcoded full-length cDNA was amplified, the products were purified and fragmented, and adapters were added. The purified cDNA was used for library construction. Last, the cDNA and library quality were measured by Qubit and Agilent Fragment Analyzer, respectively, and sequenced on an Illumina NovaSeq platform with 150-bp paired-end reads.
3
Singleron GEXSCOPE™ scRNA-Seq Protocol
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Fresh maxillary process tissues were conserved in the GEXSCOPE® Tissue Preservation Solution (Singleron) until library preparation. The scRNA-Seq libraries were constructed in accordance with the Singleron GEXSCOPE™ protocol from the GEXSCOPE™ Single-Cell RNA Library Kit (Singleron Biotechnologies). Pools were sequenced on the Illumina HiSeq X to generate 150 bp paired-end reads. Unsupervised clustering of cell populations was performed using the tSNE and UMAP analysis from the Seurat R package.
4
Single-cell RNA Sequencing Protocol
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Single-cell suspensions at 1 × 105 cells/mL in concentration in PBS (HyClone, Shanghai, China) were prepared and loaded onto microfluidic devices and scRNA-seq libraries were constructed according to Singleron GEXSCOPE® protocol by GEXSCOPE® Single-Cell RNA Library Kit (Singleron Biotechnologies) and Singleron Matrix® Automated single-cell processing system (Singleron Biotechnologies). Individual libraries were diluted to 4 ng/μL and pooled for sequencing. Pools were sequenced on Illumina HiSeq X (Illumina, San Diego, CA, USA) with 150 bp paired end reads.
5
Single-Cell RNA Sequencing Library Prep
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Single-cell suspensions with 1 × 105 cells/mL in PBS were prepared. Then, the suspensions were loaded onto microfluidic devices, and scRNA-seq libraries were constructed according to the Singleron GEXSCOPE™ protocol in the GEXSCOPE™ Single-Cell RNA Library Kit (Singleron Biotechnologies).21 (link) Individual libraries were diluted to 4 nM and pooled for sequencing. Pools were sequenced on an Illumina HiSeq X with 150 bp paired-end reads.
6
Single-Cell RNA Sequencing Library Prep
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Single-cell suspensions were prepared at a concentration of 1 × 105 cells/mL in PBS then loaded onto microfluidic devices, where scRNA-seq libraries were constructed according to the Singleron GEXSCOPE® protocol by the GEXSCOPE® Single-Cell RNA Library Kit (Singleron Biotechnologies) [24] . Individual libraries were diluted to 4 nM and pooled for sequencing. Pools were sequenced on an Illumina HiSeq X with 150 bp paired-end reads.
7
Single-cell RNA Sequencing of Midpalatal Suture
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The midpalatal suture region cell populations were scratched off from each cranial–maxillary complex sample and collected into a preservation solution in sealed tubes. The concentration of the single-cell preparations was assessed using a hemocytometer in an inverted microscope and was adjusted to 1 × 105 cells/mL in PBS (HyClone, Cytiva, Marlborough, MA, USA). Single-cell suspensions were loaded onto microfluidic devices. Then, scRNA-seq libraries were constructed on the basis of the Singleron GEXSCOPE® protocol with GEXSCOPE® Single-Cell RNA Library Kits (Singleron Biotechnologies). Individual libraries were diluted to 4 nM, and the pooled libraries were sequenced on an Illumina novaseq 6000 with 150 bp paired-end reads.
8
Single-Cell RNA Sequencing Library Preparation
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Single-cell suspensions at a concentration of 1×105 cells/mL in PBS (HyClone) were prepared. They were then loaded onto microfluidic devices, and scRNA-seq libraries were constructed according to Singleron GEXSCOPE® protocol by GEXSCOPE® Single-Cell RNA Library Kit (Singleron Biotechnologies) (Dura et al., 2019 (link)). Individual libraries were diluted to 4 nM and pooled for sequencing. Pools were sequenced on Illumina HiSeq X with 150-bp paired end reads.
9
Isolating Cranial-Maxillary Cells for Single-Cell RNA Sequencing
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The experiments in this study were carried out in compliance with the ARRIVE guidelines and were approved by the Beijing Municipal Science and Technology Commission (MDKN-2022-011). Twenty-seven healthy specific pathogen-free (SPF) female C57BL6/J mice at 4, 5, and 9 weeks of age (nine mice at each age) were selected (Beijing Vital River Laboratory Animal Technology Co., Beijing, China). The animals were sacrificed by cervical dislocation, the skin and mandible were removed, and the cranial–maxillary complex was extracted. Then, nine mice (three at 4 weeks of age, three at 5 weeks of age, and three at 9 weeks of age) were used for cell isolation. The cranial–maxillary complex of each sample was split through the midpalatal suture by a blade and tissue cells on bilateral bone surfaces inside the midpalatal suture were scraped off and put into a preserving solution (Singleron GEXSCOPE, Jiangsu, China) and prepared for single-cell RNA sequencing analysis.
10
Single-Cell RNA-seq of Colon Tissue
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The fresh colon tissues were stored in the sCelLiveTM Tissue Preservation Solution (Singleron Biotechnologies, China) at 4 °C. The scRNA-seq libraries were constructed using the GEXSCOPE® Single-Cell RNA Library Kit and Singleron Matrix® Automated single-cell processing system, following the Singleron GEXSCOPE® operation instructions. Individual libraries were diluted to 4 nM and sequenced with 150 bp paired-end reads on Illumina Novaseq6000.
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