Cyanine cy3 conjugated donkey anti rabbit igg

Manufactured by Jackson ImmunoResearch

Cyanine Cy3-conjugated donkey anti-rabbit IgG is a secondary antibody that targets rabbit immunoglobulin G (IgG) and is conjugated with the Cyanine 3 (Cy3) fluorescent dye.

Automatically generated - may contain errors

Lab products found in correlation

Cyanine cy3 conjugated donkey anti mouse igg, by Jackson ImmunoResearch (2 mentions) Ab16051, by Abcam (1 mentions) Ab25245, by Abcam (1 mentions) Ab13970, by Merck Group (1 mentions) Alexa fluor 488 conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions) A7811, by Merck Group (1 mentions) A11122, by Abcam (1 mentions) Evos fl auto cell imaging system, by Thermo Fisher Scientific (1 mentions) C6219, by Abcam (1 mentions) Alexa fluor 488 conjugated donkey anti chicken igg, by Jackson ImmunoResearch (1 mentions) Alexa fluor 647 conjugated donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions) Cyan flow cytometer, by BD (1 mentions) Accuri c6, by BD (1 mentions) Mouse monoclonal anti actin, by Merck Group (1 mentions) Hrp conjugated goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) Hrp conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Mouse monoclonal anti lamp1 h4a3, by Santa Cruz Biotechnology (1 mentions) Mouse monoclonal anti v5 r960 25, by Thermo Fisher Scientific (1 mentions) Anti chmp4b, by Proteintech (1 mentions)

2 protocols using cyanine cy3 conjugated donkey anti rabbit igg

Immunofluorescence and Flow Cytometry Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunofluorescence staining and flow cytometry were performed as previously described (18 (link)). Primary antibodies (table S5) were used at the following dilutions: rabbit anti-GFP (Invitrogen, A11122; 1:500), chicken anti-GFP (Abcam, ab13970; 1:1500), anti-αActinin (Sigma-Aldrich, A7811; 1:500), anti-Cx43 (Sigma-Aldrich, C6219; 1:200), anti-Lamp1 (Abcam, ab25245; 1:200), and anti–β-catenin (Abcam, ab16051; 1:200). Secondary antibodies including Alexa Fluor 488–conjugated donkey anti-rabbit IgG, Alexa Fluor 488–conjugated donkey anti-chicken IgG, Alexa Fluor 647–conjugated donkey anti-mouse IgG, cyanine Cy3-conjugated donkey anti-rabbit IgG, and cyanine Cy3-conjugated donkey anti-mouse IgG were all from Jackson ImmunoResearch Inc. Images were captured using EVOS FL Auto Cell Imaging System (Life Technologies). For quantification, 10 to 20 images were randomly taken under ×10 or ×20 magnifications at the same exposure setting and then counted in a double-blinded way. For flow cytometry, iCMs on d10 were harvested by trypsin digestion at 37°C for 5 min. Cells were fixed, permeabilized, probed for αMHC-GFP and cTNT, and then analyzed on a BD Accuri C6 or Cyan flow cytometer. FlowJo software (Tree Star) was used to analyze flow cytometry data.

Wang L., Ma H., Huang P., Xie Y., Near D., Wang H., Xu J., Yang Y., Xu Y., Garbutt T., Zhou Y., Liu Z., Yin C., Bressan M., Taylor J.M., Liu J, & Qian L. (2020). Down-regulation of Beclin1 promotes direct cardiac reprogramming. Science translational medicine, 12(566), eaay7856.

+ Open protocol

+ Expand

Antibody Profiling of TMEM16F, SMS2, and nSMase-2

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies used were: rabbit polyclonal anti-TMEM16F (Sigma-Aldrich, HPA038958; IB 1:1000); mouse monoclonal anti-SMS2 [7D10] (Santa Cruz, sc-293384; IB 1:1000); mouse monoclonal anti-nSMase-2 (Santa Cruz, sc-166637; IB: 1:1000); mouse monoclonal anti-LAMP1 [H4A3] (Santa Cruz, sc-20011; IF 1:200); rabbit polyclonal anti-CHMP4B (Proteintech, 13683-1-AP; IF 1:300); mouse monoclonal anti-ALIX [3A9] (Biolegend, 634501, IB 1:1000); mouse monoclonal anti-Actin (Sigma-Aldrich, A1978; IF 1:1200; IB 1:10,000); rabbit monoclonal anti-Na/K-ATPase [EP1845Y] (Abcam, ab-76020; IF 1:600); mouse monoclonal anti-TSG101 [C-2] (Santa Cruz, sc-7964; IB 1:1000); mouse monoclonal anti-V5 [R960-25] (Invitrogen, r96025; IF 1:400; IB 1:1000); rabbit polyclonal anti-GFP (Novus Biologicals, NB600-303; IF 1:250); HRP-conjugated goat anti-mouse IgG (Thermo Fisher Scientific, 31430; IB 1:5000); HRP-conjugated goat anti-rabbit IgG (Thermo Fisher Scientific; 31460; IB 1:5000); Cyanine Cy™2-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch Laboratories, 715-225-150; IF 1:400); Cyanine Cy™2-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories, 711-225-152; IF 1:400); Cyanine Cy™3-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories, 715-165-152; IF 1:400); and Cyanine Cy™3-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch Laboratories, 715-165-150; IF 1:400).

Niekamp P., Scharte F., Sokoya T., Vittadello L., Kim Y., Deng Y., Südhoff E., Hilderink A., Imlau M., Clarke C.J., Hensel M., Burd C.G, & Holthuis J.C. (2022). Ca2+-activated sphingomyelin scrambling and turnover mediate ESCRT-independent lysosomal repair. Nature Communications, 13, 1875.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!