Antibodies for neuroblast (NB) and intermediate neural progenitor (INP) detection were used at the following dilutions in 0.3% PBST (PBS + 10% Triton-X 100): Rabbit anti-phospho-Histone H3 (pH3; Cell Signaling) at 1:500, rat anti-Deadpan (Abcam) at 1:75, goat anti-rabbit Cy3 (Jackson Immunoresearch) at 1:300, and goat anti-rat Alexa 488 (Jackson ImmunoResearch) at 1:250. Anti-Deadpan marks all NBs and INPs and was used to define and exclude the optic lobe during analysis. Anti-pH3 marks all proliferating NBs and progenitor cells in M phase. Antibodies used for the detection of fasciculating axons in the mushroom body were used at the following dilutions in 0.1% PBST: Mouse anti-FasciclinII (Developmental Studies Hybridoma Bank) at 1:20 and goat anti-mouse Alexa 488 (Jackson ImmunoResearch) at 1:1000.
Rabbit anti phospho histone h3 ph3
Manufactured by Cell Signaling Technology
Sourced in Germany
Rabbit anti-phospho-Histone H3 (pH3) is a primary antibody that specifically recognizes histone H3 phosphorylated at serine 10 or serine 28. This antibody can be used to detect cell proliferation and mitosis.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti rabbit cy3, by Jackson ImmunoResearch (1 mentions)
Goat anti rat alexa 488, by Jackson ImmunoResearch (1 mentions)
Rat anti deadpan, by Abcam (1 mentions)
Mouse anti fasciclin 2, by Developmental Studies Hybridoma Bank (1 mentions)
Goat anti mouse alexa 488, by Jackson ImmunoResearch (1 mentions)
Rabbit anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Rabbit anti gfp, by Santa Cruz Biotechnology (1 mentions)
Axiophot, by Zeiss (1 mentions)
Fitc or cy3, by Jackson ImmunoResearch (1 mentions)
2 protocols using rabbit anti phospho histone h3 ph3
1
Immunodetection of Neural Progenitors
2
Drosophila Genetics and Imaging Techniques
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Flies were raised on standard corn-molasses food at 25 °C. The following strains were used: da-Gal4 (BL55849); en-Gal4-GFP [46 (link)], UAS-lacZ (BL8530), UAS-mei-41 (this work), UAS-grapes.ORF.3xHA (F000934; obtained from FlyORF; Zürich, Switzerland) [47 (link)], UAS-chk2 (lok) (gift of U. Abdu) [48 (link)], UASp-lacZ [49 (link)], p53R-GFP [36 (link)]. Staining of third instar wing imaginal discs or salivary glands was done according to standard protocols as described earlier [38 (link), 50 (link)] using the following antibodies: rabbit anti-GFP (1:100; Santa Cruz Biotech, Dallas, USA), guinea pig anti-Pzg (1:1000) [39 (link)], rabbit anti-cleaved Caspase-3 (1:250; Cell Signaling, Germany) and rabbit anti-Phospho-Histone H3 (PH3) (1:50; Cell Signaling, Germany). Secondary antibodies from goat or donkey, coupled to FITC or Cy3, were obtained from Jackson Immuno-Research Laboratories (Dianova, Hamburg, Germany). Larval tissue was documented by confocal microscopy using a MRC1024 confocal scan head coupled to a Zeiss Axiophot (Carl Zeiss AG, Oberkochen, Germany) and LaserSharp 2000 imaging software. Pictures were compiled with Corel Photo Paint and Corel Draw software.
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