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6 protocols using manganese chloride

1

Simultaneous Detection of Cd(II) and Hg(II) Ions

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L-arginine and standard reserve solutions of Cd2+ (892.9 μM), and Hg2+(498.5 μM) were obtained from Aladdin (Aladdin, Shanghai, China). Potassium chloride (KCl), sodium chloride (NaCl), magnesium chloride (MgCl2), calcium chloride (CaCl2), manganese chloride (MnCl2), zinc chloride (ZnCl2), aluminum chloride (AlCl3), nickel chloride (NiCl2.6H2O), sodium nitrate (NaNO3), sodium nitrite (NaNO2), sodium dihydrogen phosphate (NaH2PO4), sodium sulfate (Na2SO4), 1,10-phenanthroline, Rochelle salt, EDTA, sodium diphosphate, phytic acid, potassium iodide, acetate buffer, and trisodium citrate dihydrate were obtained from Sinopharm Chemical Reagent (Shanghai, China). All the reagents and solvents of analytical grades were used without further purification. All the experiments were carried out using deionized water.
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2

Synthesis of Catalysts using Transition Metal Salts

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All the materials used for synthesizing catalysts, including ethylene glycol, poly(ethylene glycol) (PEG), sodium acetate (CH3COONa), ferric chloride (FeCl3·6H2O), manganese chloride (MnCl2·H2O), zinc chloride (ZnCl2), cupric chloride (CuCl2·2H2O), nickel chloride (NiCl2·6H2O), cobalt chloride (CoCl2·6H2O), ethyl alcohol (C2H6O), 5,5-dimethyl-1-pyrroline N-oxide (DMPO), were purchased from Sinopharm Chemical Reagent Co., Ltd. All chemicals were used without further purification.
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3

Wastewater Sludge Cr(VI) Removal

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Ferric chloride (FeCl3·6H2O), manganese chloride (MnCl2·5H2O), sodium hydroxide (NaOH), hydrochloric acid (HCl), and potassium dichromate (K2Cr2O7) were purchased from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). Above reagent was of analytical reagent. Different concentration of Cr(vi) solutions was obtained through K2Cr2O7 solids dissolution. Dewatering sludge from Chengdu (Sichuan Province, China) municipal wastewater treatment plant.
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4

Synthesis of Transition Metal Oxides

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All the reagents used were of analytical grade and used as received. Nickel chloride (NiCl2·6H2O), cobalt chloride (CoCl2·6H2O), ferric chloride (FeCl3), manganese chloride (MnCl2·4H2O), chromium chloride (CrCl3·6H2O), and glucose (C6H12O6) were purchased from Sinopharm Chemical Reagent Co. Ltd.
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5

Intratumoral Immune Cell Analysis

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ALG was obtained from J&K Chemicals. CaCl2 was obtained from Sigma-Aldrich. Manganese chloride (MnCl2), sodium chloride (NaCl), zinc chloride (ZnCl2), and potassium chloride (KCl) were purchased from Sinopharm Chemical Reagent Co. RPMI 1640 medium (no calcium nitrate) was purchased from HyClone. Antibodies for flow cytometric analysis of intratumoral immune cells were obtained from BioLegend and eBioscience. ELISA kits were obtained from Invitrogen.
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6

Cytoskeletal Regulation in Cell Migration

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Antibody against α-tubulin (11224-1-AP, Western blotting 1:5000, immunofluorescence 1:200) was obtained from Proteintech Group (Rosemont, IL, USA). Antibody against talin (A02859-1, Western blotting 1:500) was purchased from Boster Bio (Pleasanton, CA, USA). Fluorescein-conjugated goat anti-mouse IgG (H+L) (ZF-0312, IF 1:100) and fluorescein-conjugated goat anti-rabbit IgG (H+L) (ZF-0311, IF 1:100) were obtained from Zsgb-Bio (Beijing, China).
Ciliobrevin D, blebbistatin, and cilengitide were purchased from Aladdin (Shanghai, China). Ispinesib (SB-715992) was purchased from Beyotime Biotechnology (Jiangsu, China). Cytochalasin D (Cyto D) and nocodazole (Noc) were obtained from MilliporeSima (Burlington, MA, USA). Manganese chloride was obtained from Sinopharm (Beijing, China).
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