Chemicals: PP2 inhibitor (Tocris), propidium iodide (PI) and Hoechst stains (Biolegend), LT-1 transfection reagent (Mirus). For imaging analysis, #0030730119 (tissue culture (TC) treated) and #0030730011 (non-TC culture treated) Eppendorf 96-well plates were used. Non-TC treated plates were used for agonistic antibody adsorption. Sterile carrier-free anti-CD3 (2C11) and anti-CD28 (37.51) were obtained from Biolegend. Fluorescent anti-TCRβ (AF647) (H57–597) and anti-CD8α (PE) (53–6.7) were obtained from Biolegend. Streptavidin nanospheres were obtained from Biolegend (#480016). Biotinylated antibodies used for negative selection cocktail were obtained from Biolegend: Biotin anti-CD11b (M1/70) (#101204), Biotin anti-CD11c (N418) (#117304), Biotin anti-CD19 (6D5) (#115504), Biotin anti-CD24 (M1/69) (#101804), Biotin anti-CD45R (B220) (RA3-6B2) (#103204), Biotin anti-CD49b (DX5) (#108904), Biotin anti-TER119 (clone TER-119) (#116204), Biotin CD4 (GK1.5) (#100404). Antibodies (0.5 mg each, except for 0.1 mg anti-CD11c) were pooled and dialyzed in PBS before sterile filtration and storage at 80 °C.
Biotin cd4 gk1.5
Manufactured by BioLegend
Biotin CD4 (GK1.5) is a lab equipment product from BioLegend. It is a fluorescent-labeled antibody that binds to the CD4 surface antigen on T helper cells. The biotin label can be used for various detection and separation techniques.
Automatically generated - may contain errors
Lab products found in correlation
Propidium iodide, by BioLegend (2 mentions)
Anti cd28 37, by BioLegend (2 mentions)
Pp2 inhibitor, by BioLegend (2 mentions)
Lt 1 transfection reagent, by BioLegend (2 mentions)
Sterile carrier free anti cd3 2c11, by BioLegend (2 mentions)
Fluorescent anti tcrβ af647 h57 597, by BioLegend (2 mentions)
Anti cd8α pe 53 6.7, by BioLegend (2 mentions)
Streptavidin nanospheres, by BioLegend (2 mentions)
Biotin anti cd11b m1 70, by BioLegend (2 mentions)
Biotin anti cd11c n418, by BioLegend (2 mentions)
Biotin anti cd19 6d5, by BioLegend (2 mentions)
Biotin anti cd24 m1 69, by BioLegend (2 mentions)
Biotin anti cd45r b220 ra3 6b2, by BioLegend (2 mentions)
Biotin anti cd49b dx5, by BioLegend (2 mentions)
Biotin anti ter119 clone ter 119, by BioLegend (2 mentions)
2 protocols using biotin cd4 gk1.5
1
Isolation and Activation of Murine T Cells
2
Isolation and Activation of Murine T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chemicals: PP2 inhibitor (Tocris), propidium iodide (PI) and Hoechst stains (Biolegend), LT-1 transfection reagent (Mirus). For imaging analysis, #0030730119 (tissue culture (TC) treated) and #0030730011 (non-TC culture treated) Eppendorf 96-well plates were used. Non-TC treated plates were used for agonistic antibody adsorption. Sterile carrier-free anti-CD3 (2C11) and anti-CD28 (37.51) were obtained from Biolegend. Fluorescent anti-TCRβ (AF647) (H57–597) and anti-CD8α (PE) (53–6.7) were obtained from Biolegend. Streptavidin nanospheres were obtained from Biolegend (#480016). Biotinylated antibodies used for negative selection cocktail were obtained from Biolegend: Biotin anti-CD11b (M1/70) (#101204), Biotin anti-CD11c (N418) (#117304), Biotin anti-CD19 (6D5) (#115504), Biotin anti-CD24 (M1/69) (#101804), Biotin anti-CD45R (B220) (RA3-6B2) (#103204), Biotin anti-CD49b (DX5) (#108904), Biotin anti-TER119 (clone TER-119) (#116204), Biotin CD4 (GK1.5) (#100404). Antibodies (0.5 mg each, except for 0.1 mg anti-CD11c) were pooled and dialyzed in PBS before sterile filtration and storage at 80 °C.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!