Trypsin blue dye

Tissue culture reagents (RPMI-1640, fetal bovine serum, penicillin, streptomycin, and L-glutamine) were from Gibco BRL. The tissue culture incubator (water jacket CO₂ incubator, Forma Series II, Model 3100 Series) was from Thermo Fisher. The tissue culture workstation (Logic+ A2 hood) was from Labconco (Kansas City, MO, USA). Tissue culture plastic (flasks, plates, cell scraper) was from CytoOne (USA Scientific, Ocala, FL, USA). Cell counting reagent (trypsin blue dye), slides (dual chamber), counter (TC20 Automatic Cell Counter), and microscope (Zoe Florescent Cell Imager) were from Bio-Rad (Hercules, CA, USA). The microplate spectrophotometer (Epoch) was from BioTek Instruments (Winooski, VT, USA).

Cell cytotoxicity reagent (MTT based-In Vitro Toxicology Assay Kit), gossypol, LPS and other chemicals (chloroform, DMSO, ethanol, hexane, sodium bicarbonate, sulfuric acid) were from Sigma (St. Louis, MO). Tissue culture reagents (DMEM, MEM, fetal bovine serum, penicillin, streptomycin, L-glutamine) were from Gibco BRL (Thermo Fisher). Tissue culture incubator was water jacket CO₂ incubator, Forma Series II, Model 3100 Series (Thermo Fisher). Tissue culture workstation was Logic + A2 hood (Labconco, Kansas City, MO). Tissue culture plastic ware (flasks, plates, cell scraper) was from CytoOne (USA Scientific, Ocala, FL). Cell counting reagent (trypsin blue dye), slides (dual chamber), counter (TC20 Automatic Cell Counter) and microscope (Zoe Florescent Cell Imager) were from Bio-Rad (Hercules, CA). Molecular sieves were from USA Standard Testing Sieves (Milwaukee, WI). Microplate spectrophotometer (Epoch) was from BioTek Instruments (Winooski, VT).

Cell cytotoxicity reagent (MTT based-In Vitro Toxicology Assay Kit) and DMSO were from Sigma. Tissue culture reagents were from Gibco BRL (Thermo Fisher). Tissue culture incubator was water jacket CO₂ incubator (Thermo Fisher). Tissue culture workstation was Logic + A2 hood (Labconco, Kansas City, MO). Tissue culture plastic ware was from CytoOne (USA Scientific, Ocala, FL). Cell counting reagent (trypsin blue dye), slides (dual chamber), counter (TC20 Automatic Cell Counter) and microscope (Zoe Florescent Cell Imager) were from Bio-Rad (Hercules, CA). Microplate spectrophotometer (Epoch) was from BioTek Instruments (Winooski, VT).