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Ultrascence pico ultra western substrate

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The UltraScence Pico Ultra Western Substrate is a chemiluminescent detection reagent designed for use in Western blotting applications. It provides a sensitive and efficient method for detecting proteins of interest on a membrane.

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Lab products found in correlation

Criterion stain free imaging system, by Bio-Rad (3 mentions) Chemidoc mp imaging system, by Bio-Rad (3 mentions) Mini protean tgx stain free protein gel, by Bio-Rad (3 mentions) Ma5 32876, by Merck Group (2 mentions) Sab3701073, by Merck Group (2 mentions) Mab3408, by Merck Group (2 mentions) Trans blot turbo transfer system, by Bio-Rad (2 mentions) Mmp 1, by Santa Cruz Biotechnology (1 mentions) Timp 2, by Abcam (1 mentions) Goat anti mouse hrp, by Merck Group (1 mentions) Thbs1, by Santa Cruz Biotechnology (1 mentions) Serpine1, by Santa Cruz Biotechnology (1 mentions) Col6a1, by Santa Cruz Biotechnology (1 mentions) Goat anti rabbit hrp, by Merck Group (1 mentions) Trans blot turbo system, by Bio-Rad (1 mentions)

3 protocols using ultrascence pico ultra western substrate

1

Protein Expression Analysis Workflow

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CM were mixed with 4× Laemmli sample buffer (containing 2-mercaptoethanol) and boiled for 10 min at 95°C. 15-μl protein volumes were separated on 4–15% Mini-PROTEAN TGX stain-free protein gels (4568086; Bio-Rad), and whole proteins were detected with the Criterion Stain-free imaging system (Bio-Rad). Next, the proteins were transferred on polyvinylidene difluoride membranes using the Trans-Blot-Turbo system (Bio-Rad). The membranes were blocked in 5% nonfat dry milk/TBS-T for 1 h at RT. The following primary antibodies were diluted 1:1,000 in 5% BSA/TBS-T and incubated overnight at 4°C: MMP1 (sc-30069; Santa Cruz), SERPINE1 (sc-5297; Santa Cruz), TIMP2 (ab53730; Abcam), COL6A1 (sc-377143; Santa Cruz), FN1 (F3648; Sigma-Aldrich), and THBS1 (sc-59887; Santa Cruz). Membranes were 3× washed in TBS-T and the following secondary antibodies were diluted 1:20,000 in 2.5% nonfat dry milk/TBS-T and incubated for 1 h at RT: goat-anti-mouse-HRP (SAB3701073-2; Sigma-Aldrich) or goat-anti-rabbit-HRP (SAB3700878-1; Sigma-Aldrich). HRP was visualized by the UltraScence Pico Ultra Western Substrate (CCH345-B; GeneDireX) and the ChemiDoc MP imaging system (Bio-Rad).
Blache U., Horton E.R., Xia T., Schoof E.M., Blicher L.H., Schönenberger A., Snedeker J.G., Martin I., Erler J.T, & Ehrbar M. (2019). Mesenchymal stromal cell activation by breast cancer secretomes in bioengineered 3D microenvironments. Life Science Alliance, 2(3), e201900304.
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2

Western Blot Analysis of PIEZO1

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Cells were washed with PBS and lysed directly in the cell culture dish with 80 μl of 1x reduced Laemmli buffer (Fisher Scientific 15493939) and boiled for 5 min at 95°C. 15 μl of each sample was loaded onto a 4-15% Mini-PROTEAN TGX stain-free protein gel (Bio-Rad 4568086). Total protein was analysed using the Criterion Stain-free imaging system (Bio-Rad) and subsequently transferred on polyvinylidene difluoride membranes using the Trans-Blot-Turbo Transfer System (Bio-Rad). Membrane blocking was carried out with 5% nonfat dry milk/TBS-T for 1 h at room temperature. The primary antibodies targeting PIEZO1 (Thermo Fisher Scientific MA5-32876, 1:500) and β-tubulin (MERCK Millipore MAB3408, 1:10’000) were diluted in 5% BSA/TBS-T and incubated overnight at 4° C. Next, the membranes were washed 3 times in TBS-T and incubated with the secondary antibody (anti-mouse, Sigma-Aldrich SAB3701073, 1:20’000) for 1 h at room temperature. Images were taken using UltraScence Pico Ultra Western Substrate (GeneDireX CCH345-B) and the ChemiDoc MP imaging system (Bio-Rad).
Passini F.S., Jaeger P.K., Saab A.S., Hanlon S., Chittim N.A., Arlt M.J., Ferrari K.D., Haenni D., Caprara S., Bollhalder M., Niederöst B., Horvath A.N., Götschi T., Ma S., Passini-Tall B., Fucentese S.F., Blache U., Silván U., Weber B., Silbernagel K.G, & Snedeker J.G. (2021). Shear-stress sensing by PIEZO1 regulates tendon stiffness in rodents and influences jumping performance in humans. Nature biomedical engineering, 5(12), 1457-1471.
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3

Western Blot Analysis of PIEZO1

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed with PBS and lysed directly in the cell culture dish with 80 μl of 1x reduced Laemmli buffer (Fisher Scientific 15493939) and boiled for 5 min at 95°C. 15 μl of each sample was loaded onto a 4-15% Mini-PROTEAN TGX stain-free protein gel (Bio-Rad 4568086). Total protein was analysed using the Criterion Stain-free imaging system (Bio-Rad) and subsequently transferred on polyvinylidene difluoride membranes using the Trans-Blot-Turbo Transfer System (Bio-Rad). Membrane blocking was carried out with 5% nonfat dry milk/TBS-T for 1 h at room temperature. The primary antibodies targeting PIEZO1 (Thermo Fisher Scientific MA5-32876, 1:500) and β-tubulin (MERCK Millipore MAB3408, 1:10’000) were diluted in 5% BSA/TBS-T and incubated overnight at 4° C. Next, the membranes were washed 3 times in TBS-T and incubated with the secondary antibody (anti-mouse, Sigma-Aldrich SAB3701073, 1:20’000) for 1 h at room temperature. Images were taken using UltraScence Pico Ultra Western Substrate (GeneDireX CCH345-B) and the ChemiDoc MP imaging system (Bio-Rad).
Passini F.S., Jaeger P.K., Saab A.S., Hanlon S., Chittim N.A., Arlt M.J., Ferrari K.D., Haenni D., Caprara S., Bollhalder M., Niederöst B., Horvath A.N., Götschi T., Ma S., Passini-Tall B., Fucentese S.F., Blache U., Silván U., Weber B., Silbernagel K.G, & Snedeker J.G. (2021). Shear-stress sensing by PIEZO1 regulates tendon stiffness in rodents and influences jumping performance in humans. Nature biomedical engineering, 5(12), 1457-1471.
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