Anti atrx

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-ATRX is a laboratory product that detects the ATRX protein. ATRX is a chromatin remodeling protein involved in various cellular processes. The Anti-ATRX product can be used to identify and quantify the ATRX protein in biological samples.

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Lab products found in correlation

Anti histone h3, by Abcam (3 mentions) A21206, by Thermo Fisher Scientific (2 mentions) A31573, by Merck Group (2 mentions) Sa5 35571, by Thermo Fisher Scientific (2 mentions) Ab96051, by Abcam (2 mentions) Anti hira, by Merck Group (2 mentions) A31572, by Merck Group (2 mentions) A31570, by Merck Group (2 mentions) A21202, by Merck Group (2 mentions) Sc 50509, by Proteintech (2 mentions) Anti histone h3, by Cell Signaling Technology (2 mentions) Anti gapdh, by Cell Signaling Technology (2 mentions) Anti β actin, by Merck Group (2 mentions) Mouse anti gfp, by Merck Group (2 mentions) Anti α tubulin, by Santa Cruz Biotechnology (2 mentions) Anti icp0, by Santa Cruz Biotechnology (2 mentions) Rabbit anti gfp, by Abcam (2 mentions) Anti ha, by Abcam (2 mentions) Anti ifi16, by Santa Cruz Biotechnology (2 mentions) Anti slfn5, by Merck Group (2 mentions)

Close spelling variants of this product

Anti-ATRX antibody (4 citations) Anti-ATRX (H-300; (2 citations) Anti ATRX (sc-15408) (2 citations)

12 protocols using anti atrx

SDS-PAGE and Immunoblotting Protocol

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Cell were lysed
into 2% w/v SDS (50 mM Tris, pH 6.8). Lysates were separated using
discontinuous SDS-PAGE to resolve proteins of molecular weight <
200 kDa or tris-acetate gels (NuPAGE, Invitrogen) to resolve proteins
of molecular weight > 200 kDa. Antibodies used for immunoblotting
were anti-SMARCAL1 (1/100, D3P5I; Cell Signaling), anti-DAXX (1/1000,
25C12; Cell Signaling), anti-ATRX (1/1000, D1N2E; Cell Signaling),
anti-ATRX (1/100, sc-55584; Santa Cruz Biotechnology), anti-alpha-tubulin
(1/5000, DM1A; Sigma-Aldrich), and anti-GAPDH (1/5000, ab9485; Abcam).

Goncalves T., Zoumpoulidou G., Alvarez-Mendoza C., Mancusi C., Collopy L.C., Strauss S.J., Mittnacht S, & Tomita K. (2020). Selective Elimination of Osteosarcoma Cell Lines with Short Telomeres by Ataxia Telangiectasia and Rad3-Related Inhibitors. ACS Pharmacology & Translational Science, 3(6), 1253-1264.

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Immunofluorescence Staining of DNA Damage Proteins

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Cells grown on coverslips were prepared for IF by standard procedures. The following antibodies were used for immunostaining: anti-ATRX (Santa Cruz sc-15408); anti-ATRX 39f [38] (link); anti MRE11 (Abcam ab214); anti-MRE11 (Calbiochem PC388); anti-RAD50 (Abcam ab89); anti-PCNA (Santa Cruz sc-9857); anti-RPA32 (S33) (Bethyl A300-246A); anti-NBS1 (BD Biosciences 611871); anti-53BP1 (Novus Biologicals NB100-305); For MRN co-localisation studies cells were pre-permeabilised prior to fixation with ice cold 0.5% Triton X-100. For MRE11/ATRX/PCNA co-localisation in mES cells, cells were attached to coverslips via poly-l-lysine treatment prior to pre-permeabilisation. Telomere FISH was performed subsequent to antibody incubation using Telomere PNA FISH Kit/Cy3 (Dako K5326).

Clynes D., Jelinska C., Xella B., Ayyub H., Taylor S., Mitson M., Bachrati C.Z., Higgs D.R, & Gibbons R.J. (2014). ATRX Dysfunction Induces Replication Defects in Primary Mouse Cells. PLoS ONE, 9(3), e92915.

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Antibody Validation for Immunofluorescence and Western Blotting

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The following antibodies were used for immunofluorescence or western blotting: Primary rabbit polyclonal: anti-actin (Sigma-Aldrich, A5060), anti-Daxx (Upstate, 07–471), anti-ATRX (Santa Cruz, H300), anti-PML (Bethyl Laboratories, A301-167A; Jena Biosciences, ABD-030), anti-Sp100 (GeneTex, GTX131569), anti-Mx1 (Santa Cruz, sc-50509; ProteinTech, 13750-1-AP), anti-ISG15 (ProteinTech, 15981-1-AP), anti-ISG54 (IFIT2, proteinTech, 12604-1-AP), and anti-histone H3 (abcam, ab1791). Primary mouse monoclonal: anti-HIRA (Millipore, 04–1488), anti-ICP0 (11060, [114 (link)]), anti-ICP4 (58s, [115 (link)]), anti-VP5 (DM165, [116 (link)]) anti-UL42 (Z1F11; [117 (link)]), and anti-PML (abcam, ab96051). Primary antibodies were detected using the following secondary antibodies: DyLight-680 or -800 conjugated goat anti-rabbit or -mouse (Thermo; 35568 and SA5-35571), Alexa -488, -555, or -647 conjugated donkey anti-rabbit or -mouse (Invitrogen; A21206, A21202, A31572, A31570, A31573, A31571), or HRP conjugated goat anti-mouse (Sigma-Aldrich, A4416).

McFarlane S., Orr A., Roberts A.P., Conn K.L., Iliev V., Loney C., da Silva Filipe A., Smollett K., Gu Q., Robertson N., Adams P.D., Rai T.S, & Boutell C. (2019). The histone chaperone HIRA promotes the induction of host innate immune defences in response to HSV-1 infection. PLoS Pathogens, 15(3), e1007667.

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DNA Damage Response Assay Protocol

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Mirin (Selleckchem, SML1839; Sigma-aldrich, M9948), PFM01 (Sigma-Aldrich, SML1735), PFM39(Sigma-Aldrich, SML1839), Talazoparib (Selleckchem, S7048), aphidicolin (Sigma-Aldrich, A0781), CD437 (Sigma-Aldrich, C5865), Agarose (Sigma-Aldrich, A9539), SSC buffer (Sigma-Aldrich, S6639), CDP-star (Roche, 11685627001), Blocking reagent (Roche, 11096176001), PBS (Genesee, 25–507B), Glycogen (GlycoBlue Coprecipitant, Invitrogen, AM9515), anti-Beta actin (abcam, ab125096), anti-Histone H3 (Cell signaling, 2650), anti-ATRX (Santa Cruz, sc-15408), anti-hMRE-11 (Calbiochem, PC388), anti-GAPDH (Cell signaling, 97166), TB Green Premix Ex Taq II (Tli RNase H Plus) (Takara, #RR820A), RevertAid First Strand cDNA synthesis kit (Thermo scientific, K1621), Lipofectamine 3000 (Thermo scientific, L3000001), TransIT (Mirus, MIR2300), Qubit dsDNA HS Assay Kit (Invitrogen, Q32851), Nylon Membranes-positively charged, (Roche, 11417240001), exo^‒ Klenow fragment (NEB, M0212), T4 DNA polymerase (NEB, M0203), Lambda exonuclease (NEB, M0262), DIG-dUTP (biorbyt, orb533128), NxGen phi29 (Biosearch Technologies, 30221)

Lee J., Lee J., Sohn E.J., Taglialatela A., O’Sullivan R.J., Ciccia A, & Min J. (2023). Extrachromosomal Telomeres Derived from Excessive Strand Displacements. bioRxiv.

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Comprehensive Antibody Characterization Protocol

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The following antibodies were used: anti-SLFN5 (Sigma-Aldrich; Cat.HPA017760; Lot.B96361), anti-SLFN11 (Novus Biologicals; Cat.NBP1–92368; Lot.H96783), anti-PML (Bethyl Laboratory; Cat.A301–167A, Santa Cruz; Cat.sc-966; Lot.H1413), anti-IFI16 (Santa Cruz; Cat.sc-8023; Lot.C1312), anti-ATRX (Santa Cruz; Cat.sc-15408), anti-DNA-PKcs (Santa Cruz; Cat.sc-5282; Lot.G280), anti-SUMO2+3 (Abcam; Cat.ab3742; Lot.GR8249–1), anti-RNAP II (Santa Cruz; Cat.sc-56767), anti-GFP rabbit (Abcam; Cat.ab290; Lot.GR3251545–1), anti-GFP mouse (Millipore; Cat.MAB2510; Lot.2512480), anti-RAD50 (GeneTex; Cat.GTX70228; Lot.40186), anti-V5 (Santa Cruz; Cat.sc-271944; Lot.E2217), anti-HA (Abcam; Cat.ab9110; Lot.GR3217183–2), anti-GAPDH (GeneTex; Cat.GTX100118; Lot.42158), anti-α-Tubulin (Santa Cruz; Cat.sc-69969; Lot.DO412), anti-β-Actin (Sigma-Aldrich; Cat.a5441; Lot.064M4789V), anti-KU70 (Abcam; Cat.ab83501; Lot.GR3176811–2), anti-Histone H3 (Abcam; Cat.ab1791; Lot.GR3198176–1), anti-ICP0 (Santa Cruz; Cat.sc-53070; Lot. A0313), anti-ICP8 (gifted from David M. Knipe), anti-TK (Santa Cruz; Cat.sc-28037; Lot.K1813), anti-VP21 and anti-gD (gifted from Gary H. Cohen), anti-IE1/IE2 (Virusys; Cat.P1215; Lot.A1345070), anti-UL44 (Virusys; Cat.ca006–100; Lot.C1034151), adenovirus late protein antibody staining Hexon, Penton and Fiber (gift from James M. Wilson), and anti-DBP (gift from Arnold J. Levine).

Kim E.T., Dybas J.M., Kulej K., Reyes E.D., Price A.M., Akhtar L.N., Orr A., Garcia B.A., Boutell C, & Weitzman M.D. (2021). Comparative proteomics identifies Schlafen 5 (SLFN5) as a herpes simplex virus restriction factor that suppresses viral transcription. Nature microbiology, 6(2), 234-245.

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Western Blot Analysis of Chromatin Regulators

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Cells were lysed with RIPA buffer supplemented with protease inhibitor cocktail and PMSF. A quantity of 50 μg cell lysate was loaded into a SDS–PAGE gel and transferred onto a polyvinylidene difluoride membrane (Bio-Rad). The membrane was blocked with 5% skim milk at room temperature for 1 h, followed by overnight incubation with primary antibodies at 4 °C. The blot was subsequently incubated with either horseradish peroxidase (HRP)-conjugated anti-mouse IgG, HRP-conjugated anti-rabbit IgG, or HRP-conjugated anti-goat IgG (1:10,000, Beith). Signal was detected using SuperSignal West Dura Extended Duration Substrate (Thermo Scientific) and captured on CLXposure film (Thermo Scientific).
The primary antibodies used for the western blot were anti-hemagglutinin (HA) (1:1000, Santa Cruz #sc-7392), anti- Actin (1:1000, Santa Cruz #sc-10731), anti-Hira (1:2000, Millipore #04–1488), anti-Daxx (1:1000, Santa Cruz #sc-7152), anti-Atrx (1:500, Santa Cruz #sc-15408) anti-H3.1/H3.2 (1:1000, Millipore #ABE154) and anti-H3.3 (1:500, Millipore #17–10245).

Fang H.T., EL Farran C.A., Xing Q.R., Zhang L.F., Li H., Lim B, & Loh Y.H. (2018). Global H3.3 dynamic deposition defines its bimodal role in cell fate transition. Nature Communications, 9, 1537.

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Antibody Characterization for SLFN5, SLFN11, and DNA Repair

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The following antibodies were used: anti-SLFN5 (Sigma-Aldrich; Cat.HPA017760; Lot.B96361), anti-SLFN11 (Novus Biologicals; Cat.NBP1-92368; Lot.H96783), anti-PML (Bethyl Laboratory; Cat.A301-167A, Santa Cruz; Cat.sc-966; Lot.H1413), anti-IFI16 (Santa Cruz; Cat.sc-8023; Lot.C1312), anti-ATRX (Santa Cruz; Cat.sc-15408), anti-DNA-PKcs (Santa Cruz; Cat.sc-5282; Lot.G280), anti-SUMO2+3 (Abcam; Cat.ab3742; Lot.GR8249-1), anti-RNAP II (Santa Cruz; Cat.sc-56767), anti-GFP rabbit (Abcam; Cat.ab290; Lot.GR3251545-1), anti-GFP mouse (Millipore; Cat.MAB2510; Lot.2512480), anti-RAD50 (GeneTex; Cat.GTX70228; Lot.40186), anti-V5 (Santa Cruz; Cat.sc-271944; Lot.E2217), anti-HA (Abcam; Cat.ab9110; Lot.GR3217183-2), anti-GAPDH (GeneTex; Cat.GTX100118; Lot.42158), anti-α-Tubulin (Santa Cruz; Cat.sc-69969; Lot.DO412), anti-β-Actin (Sigma-Aldrich; Cat.a5441; Lot.064M4789V), anti-KU70 (Abcam; Cat.ab83501; Lot.GR3176811-2), anti-Histone H3 (Abcam; Cat.ab1791; Lot.GR3198176-1), anti-ICP0 (Santa Cruz; Cat.sc-53070; Lot. A0313), anti-ICP8 (gifted from David M. Knipe), anti-TK (Santa Cruz; Cat.sc-28037; Lot.K1813), anti-VP21 and anti-gD (gifted from Gary H. Cohen), anti-IE1/IE2 (Virusys; Cat.P1215; Lot.A1345070), anti-UL44 (Virusys; Cat.ca006-100; Lot.C1034151), adenovirus late protein antibody staining Hexon, Penton and Fiber (gift from James M. Wilson), and anti-DBP (gift from Arnold J. Levine).

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Antibody panel for protein detection

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The following antibodies were used for immunofluorescence or western blotting: Primary rabbit polyclonal: anti-actin (Sigma-Aldrich, A5060), anti-Daxx (Upstate, 07–471), anti-ATRX (Santa Cruz, H300), anti-PML (Bethyl Laboratories, A301-167A; Jena Biosciences, ABD-030), anti-Sp100 (GeneTex, GTX131569), anti-SUMO2/3 (Abcam, ab22654), anti-Mx1 (Santa Cruz, sc-50509;ProteinTech, 13750-1-AP), anti-ISG15 (ProteinTech, 15981-1-AP), and anti-ISG54 (IFIT2, proteinTech, 12604-1-AP). Primary mouse monoclonal: anti-ICP0 (11060, [92 (link)]), anti-ICP4 (58s, [93 (link)]), anti-VP5 (DM165, [94 (link)]), anti-SUMO2/3 (Abcam, ab81371), anti-PML (abcam, ab96051), anti-IFI16 (abcam, ab55328; Santa Cruz, sc-8023). Primary antibodies were detected using the following secondary antibodies: DyLight-680 or -800 conjugated anti-rabbit or -mouse (Thermo; 35568 and SA5-35571), Alexa -488, -555, or -647 conjugated anti-rabbit, or -mouse (Invitrogen; A21206, A21202, A31572, A31570, A31573, A31571), HRP conjugated anti-mouse (Sigma-Aldrich, A4416).

Alandijany T., Roberts A.P., Conn K.L., Loney C., McFarlane S., Orr A, & Boutell C. (2018). Distinct temporal roles for the promyelocytic leukaemia (PML) protein in the sequential regulation of intracellular host immunity to HSV-1 infection. PLoS Pathogens, 14(1), e1006769.

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Western Blot for Protein Analysis

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The following antibodies were used for Western Blot: anti-Beta actin (abcam, ab125096), anti-Histone H3 (Cell signaling, 2650), anti-ATRX (Santa Cruz, sc-15408), anti-hMRE-11 (Calbiochem, PC388), anti-GAPDH (Cell signaling, 97166)

Lee J., Lee J., Sohn E.J., Taglialatela A., O’Sullivan R.J., Ciccia A, & Min J. (2023). Extrachromosomal Telomeres Derived from Excessive Strand Displacements. bioRxiv.

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Chromatin Immunoprecipitation Protocol

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Antibodies used were directed against anti-H3 (Abcam, #ab1791), anti-H3.3 (Abcam, #ab176840), anti-H3K9me3 (Abcam, ab8898), anti-H3K36me3 (Abcam, #ab9050), anti-γH2A.X/phospho-histone H2A.X (Ser139) clone JBW301 (Merck Millipore, #05-636), anti-ATRX (Santa Cruz Biotechnologies, #sc15408), anti- KDM4B (Abcam, #ab191434) anti-IDH1 (Sigma Aldrich, SAB4100064), anti-IDH1^R132H (Sigma Aldrich, SAB4200548), anti-HP1α (Merck Millipore, #MAB3584), anti-PML (Merck Millipore, #MAB3738), anti-TP53 (Cell Signaling Technologies, #cst-2524), anti-Flag (Sigma, #F1804), anti-TERF1 (Alpha Diagnostics, #TRF12-S) and anti-BrdU (Abcam, #ab6326), Anti-β Actin (AC-15) (Santa Cruz Biotechnology, #sc69879), Goat anti Rabbit IgG, HRP conjugate (Merck Millipore, #AP187P), Donkey anti-Mouse IgG HRP conjugate (Merck Millipore, #AP192P), Donkey anti-Mouse IgG (H + L) Alexa Fluor 594 (Invitrogen, #A-21203), Donkey anti-Rabbit IgG (H + L) Alexa Fluor 488 (Invitrogen, #A-21206).

Udugama M., Hii L., Garvie A., Cervini M., Vinod B., Chan F.L., Das P.P., Mann J.R., Collas P., Voon H.P, & Wong L.H. (2021). Mutations inhibiting KDM4B drive ALT activation in ATRX-mutated glioblastomas. Nature Communications, 12, 2584.

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