Cells one-day post-treatment were harvested and stained for CD14, CD36, and NRP-1 (BioLegend). Cells were analyzed on a BD FACS Fortessa Flow cytometer (BD Biosciences). Expression analysis was performed using FlowJo (FlowJo, LLC).
Nrp 1
Manufactured by BioLegend
Sourced in United States
NRP-1 is a cell surface receptor protein involved in the regulation of various biological processes. It plays a key role in axon guidance, angiogenesis, and immune cell function. The core function of NRP-1 is to act as a co-receptor for other signaling molecules, facilitating their interactions and downstream cellular responses.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nrp 1
1
Multiparameter Flow Cytometry Analysis
2
Phenotypic Characterization of Regulatory T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Each 7th and 14th day of the expansion samples of Tregs37, Tregs33 and Teffs were labeled with Abs against the following antigens (Ag): CD4, CD25, CD127, CD45RA (BD Biosciences, USA), CD62L (Life Technologies, USA), Nrp-1 (BioLegend, USA), HSP-60, HSP-70, HSP-90 (Abcam, UK), CD39, CTLA-4, Helios and FoxP3 using Foxp3 Staining Buffer Set (eBioscience, USA) and analyzed with flow cytometry (Canto II, BD Biosciences, USA). As it was reported before7 (link),34 Tregs were assigned to naive (Tn), central memory (Tcm) and effector memory (Tem) populations according to the following phenotypes: CD4+FoxP3+CD45RA+CD62L+, CD4+FoxP3+CD45RA-CD62L+ and CD4+FoxP3+CD45RA-CD62L−, respectively.
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