Anti cleaved casp8

Manufactured by Cell Signaling Technology

Anti-cleaved-CASP8 is a primary antibody that specifically detects the cleaved form of caspase-8, a key enzyme involved in the apoptosis (programmed cell death) signaling pathway. This antibody can be used to monitor caspase-8 activation in cellular assays.

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Lab products found in correlation

Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Jsh 23, by Selleck Chemicals (1 mentions) Anti β actin, by Bioworld Technology (1 mentions) Nlrc4, by ABclonal (1 mentions) Anti phospho nf kb p65, by Cell Signaling Technology (1 mentions) Hif 1α, by ABclonal (1 mentions) Il 1β, by ABclonal (1 mentions) Nlrp3, by ABclonal (1 mentions) Gsdmd, by ABclonal (1 mentions) Tissuelyser 2, by Qiagen (1 mentions) Odyssey fc, by LI COR (1 mentions) Anti α tubulin, by Merck Group (1 mentions) Anti cleaved casp3, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Casp8 (5 citations)

2 protocols using anti cleaved casp8

Antibody-mediated Regulation of NLRP3 and NF-κB Signaling

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Antibodies targeting the following proteins were purchased from ABclonal Biotech Co., Ltd. (USA): HIF-1α (#A11945), HIF-1α (#A1544), NLRP3 (#A6345), NLRP12 (#A6671), NLRC4 (#A7382), CASP1 (#A0964), CASP8 (#A0215), IL-1β (#A1112) and GSDMD (#A10164). Anti-cleaved-CASP8 (#8592S) and anti-phospho-NF-kB P65 (#3033S) antibodies were obtained from Cell Signaling Technology. An IL-1β neutralizing antibody (#14–7012-85) was obtained from eBioscience. The CASP1 inhibitor (CASP1 inh) Z-YVAD-FMK (YVAD, #ab141388) was obtained from Abcam. The NF-kB P65 inhibitor JSH-23 (#S7351) was purchased from Selleckchem. Anti-β-actin (#AP0060) and secondary antibodies were purchased from Bioworld Technology. Lipofectamine 3000 was purchased from Invitrogen.

Chen H., Deng Y., Gan X., Li Y., Huang W., Lu L., Wei L., Su L., Luo J., Zou B., Hong Y., Cao Y., Liu Y, & Chi W. (2020). NLRP12 collaborates with NLRP3 and NLRC4 to promote pyroptosis inducing ganglion cell death of acute glaucoma. Molecular Neurodegeneration, 15, 26.

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Protein Analysis of Liver Tissue

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Liver tissue was smashed using a tissue lyser (TissueLyser II; Qiagen) and lysed using sodium dodecyl sulfate (SDS) lysis buffer (1.1% SDS, 11% glycerol, 0.1 mol/L Tris, pH 6.8) with 10% β‐mercaptoethanol. Blots were probed with anti‐α‐tubulin (Merck, Kenilworth, NJ), anti‐cleaved Casp3, anti‐cleaved Casp8, or anti‐beta‐2 microglobulin (Cell Signaling) followed by detection with the Odyssey infrared imaging system (Odyssey Fc; LI‐COR Biosciences, Lincoln, NE). Immunoblots were quantified using ImageJ.

Xu H.C., Huang J., Pandyra A.A., Pandey P., Wang R., Zhang Z., Zhuang Y., Gertzen C.G., Münk C., Herebian D., Borkhardt A., Recher M., Gohlke H., Esposito I., Oberbarnscheidt M., Häussinger D., Lang K.S, & Lang P.A. (2022). Single MHC‐I Expression Promotes Virus‐Induced Liver Immunopathology. Hepatology Communications, 6(7), 1620-1633.

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