The identification of surface molecules was carried out by incubating the cells with human monoclonal APC-conjugated anti-toll like receptor 4 (TLR4), FITC-conjugated CD14, and PE-conjugated CD86 antibodies (all from Biolegend, San Diego, CA, USA) for 20 min at 4 °C, according to the manufacturer’s recommendations. Cells were washed with 1X PBS and fixed with 4% paraformaldehyde (Sigma-Aldrich, Inc.) for 20 min at room temperature, washed, and acquired with a BD FACSVerse Flow Cytometer (BD Biosciences, San Jose, CA, USA). The results were analyzed using Flow Jo ver. 10 (Beckton Dickinson, East Rutherford, NJ, USA).
Pe conjugated cd86
PE-conjugated CD86 is a laboratory reagent that consists of the CD86 antibody conjugated to the fluorescent dye Phycoerythrin (PE). CD86 is a cell surface protein that is expressed on antigen-presenting cells and plays a role in T cell activation and immune response. The PE-conjugated CD86 antibody can be used to identify and study CD86-expressing cells in research applications.
Lab products found in correlation
3 protocols using pe conjugated cd86
Flow Cytometric Analysis of ARPE-19 Cell Surface Markers
The identification of surface molecules was carried out by incubating the cells with human monoclonal APC-conjugated anti-toll like receptor 4 (TLR4), FITC-conjugated CD14, and PE-conjugated CD86 antibodies (all from Biolegend, San Diego, CA, USA) for 20 min at 4 °C, according to the manufacturer’s recommendations. Cells were washed with 1X PBS and fixed with 4% paraformaldehyde (Sigma-Aldrich, Inc.) for 20 min at room temperature, washed, and acquired with a BD FACSVerse Flow Cytometer (BD Biosciences, San Jose, CA, USA). The results were analyzed using Flow Jo ver. 10 (Beckton Dickinson, East Rutherford, NJ, USA).
Flow Cytometric Analysis of Dendritic Cell Maturation
Immunophenotypic Characterization of Cells
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