Em ccd camera

Manufactured by Zeiss

Sourced in United Kingdom, Germany

The EM-CCD camera is a scientific imaging device designed for low-light applications. It utilizes an electron-multiplying charge-coupled device (EM-CCD) sensor to provide high-sensitivity detection of photons. The EM-CCD camera is capable of capturing images and video in a wide range of scientific and research fields.

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Lab products found in correlation

Axio observer, by Zeiss (1 mentions) Custom 100x 1.46 na oil antiflex objective, by Zeiss (1 mentions) Inverted fluorescence microscopy, by Zeiss (1 mentions) Lab tek 2 chamber coverglass, by Thermo Fisher Scientific (1 mentions) Illustrator cs6, by Adobe (1 mentions) Dm5000b, by Leica (1 mentions) Dfc500, by Leica (1 mentions) Axiocam 506, by Zeiss (1 mentions)

Spelling variants of this product

CCD camera (78 citations) ORCA EM-CCD camera (3 citations) EM‐CCD (2 citations)

4 protocols using em ccd camera

Visualizing Cell-Surface Interactions

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Total internal reflection microscopy (TIRFM) and reflection interference contrast microscopy (RICM) were performed using an inverted microscope (AxioObserver, Zeiss, Germany), equipped with an EM-CCD camera (iXon, Andor, UK). Acquisition was performed using Andor iQ, or Zeiss ZEN software. TIRF and RICM images were taken with a 100X 1.45 NA oil or a custom 100X 1.46 NA oil antiflex objective (Zeiss). For TIRF exposure time was 100 ms, and fluorescence filter sets adapted to the fluorophores were used. For RICM exposure time was 200 ms.

Benard E., Nunès J.A., Limozin L, & Sengupta K. (2018). T Cells on Engineered Substrates: The Impact of TCR Clustering Is Enhanced by LFA-1 Engagement. Frontiers in Immunology, 9, 2085.

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Optogenetic Calcium Imaging of Synaptic Responses

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Primary hippocampal neurons were transduced with AAV2/1.hSynapsin1.SF-iGluSnFR.S72A or AAV2/1.hSynapsin1.SF-iGluSnFR.A184V on DIV4 and imaged on DIV13. A low amplitude field stimulation (1 msec, 20mA, platinum bar electrodes) was applied to recruit a small fraction (~20%) of neurons. Images were acquired with an EM-CCD camera (frame time 5–50 msec) and a stabilized LED light source of cultures visualized through a coverslip with high NA objective (Zeiss, 63×, 1.4 NA, water). All experiments were performed in Tyrode’s solution (1 mL/min) at RT. Low and high affinity versions of SF-iGluSnFR were expressed in a comparable manner.

Marvin J.S., Scholl B., Wilson D.E., Podgorski K., Kazemipour A., Müller J.A., Schoch S., Quiroz F.J., Rebola N., Bao H., Little J.P., Tkachuk A.N., Cai E., Hantman A.W., Wang S.S., DePiero V.J., Borghuis B.G., Chapman E.R., Dietrich D., DiGregorio D.A., Fitzpatrick D, & Looger L.L. (2018). Stability, affinity and chromatic variants of the glutamate sensor iGluSnFR. Nature methods, 15(11), 936-939.

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Live Imaging of Chromosome Dynamics

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The cell line U-2 OS H2B-RFP, which expresses Histone 2B fused to red fluorescent protein, was used to observe chromosome dynamics in live cells. Cells (25 000 cells/well) were seeded in four-well plates adapted to video-microscopy (Lab-Tek II Chamber coverglass, Thermo Fisher Scientific, USA) for 24 h. Cells were exposed to P3 or DMSO for 24 h. During the treatment time, cells were visualized under an inverted fluorescence microscopy (Zeiss, Germany) and images were recorded every 20 min by an EMCCD camera (Zeiss, Germany). Images were analyzed by MetaMorph^® Software (MetaMorph Inc., USA).

Nguyen T.N., Feizbakhsh O., Sfecci E., Baratte B., Delehouzé C., Garcia A., Moulin C., Colas P., Ruchaud S., Mehiri M, & Bach S. (2019). Kinase-Based Screening of Marine Natural Extracts Leads to the Identification of a Cytotoxic High Molecular Weight Metabolite from the Mediterranean Sponge Crambe tailliezi. Marine Drugs, 17(10), 569.

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Microscopic Imaging: Protocols and Analysis

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Whole-mount tissues and cryosections were imaged at room temperature on one of two microscopes: an inverted Zeiss confocal microscope with a Yokogawa CSU-10 spinning disc and Andor iXon EMCCD camera using a Zeiss Plan-NeoFluar 40x (NA: 1.3) 1022–818 oil immersion objective or with an inverted Zeiss Spinning Disc with a Yokogawa CSU-X1 spinning disc and Axiocam 506 CCD camera using a Zeiss 40x (NA: 1.1) LD C-Apochromat water immersion objective. FFPE sections were imaged on an upright Leica DM5000B microscope with a Leica DFC 500 camera and using 40x (NA: 0.75) HCX PL S-APO air objective or a 63x (NA: 1.3) HCX PL APO oil objective. All image analysis was performed using FIJI (Schindelin et al., 2015 (link)). Figures were created using Adobe Illustrator CS6.

Jones K.B., Furukawa S., Marangoni P., Ma H., Pinkard H., D’Urso R., Zilionis R., Klein A.M, & Klein O.D. (2018). Quantitative clonal analysis and single cell transcriptomics reveal division kinetics, hierarchy and fate of oral epithelial progenitor cells. Cell stem cell, 24(1), 183-192.e8.

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