Live dead near ir dead cell kit

Manufactured by Thermo Fisher Scientific

The Live Dead Near-IR Dead Cell kit is a fluorescence-based assay designed to quantify live and dead cells in a sample. The kit utilizes a near-infrared dye that selectively stains dead cells, allowing for their detection and enumeration alongside live cells.

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Lab products found in correlation

Cd8 pe cy7, by BioLegend (2 mentions) Cd25 pe cy7, by BD (1 mentions) Cd45 bv510, by BioLegend (1 mentions) Cd3 bv785, by BioLegend (1 mentions) Ifnγ bv421, by BioLegend (1 mentions) Cd25 bv421, by BioLegend (1 mentions) Lsrii flow cytometer, by BD (1 mentions) Cd69 fitc, by BD (1 mentions) Cd3 apc, by BioLegend (1 mentions) Cd3 apc cy7, by BioLegend (1 mentions) Ctla 4 bv421, by BioLegend (1 mentions)

Spelling variants of this product

LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit (301 citations) Live/Dead Near-IR (53 citations) LIVE/DEAD fixable Near-IR Dead Cell staining kit (8 citations) Live/Dead Near-IR Dead cell stain kit (7 citations) LIVE/DEAD fixable near-infrared (IR) dead cell stain kit (4 citations) LIVE/DEAD Fixable Near-IR cell stain kit (4 citations) Live/Dead fixable near-IR or blue dead cell staining kit (2 citations) LIVE/DEAD Fixable Violet or Near-IR Dead Cell Stain kit (2 citations) LIVE-DEAD® Fixable yellow or near-IR dead cell stain kit (2 citations)

2 protocols using live dead near ir dead cell kit

Immunophenotyping of Lymphocytes

Cited 1 time

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Cell staining, flow cytometry and intracellular staining was performed as previously described. [26 (link)] Isolated spleen and liver infiltrating lymphocytes were stained with Live Dead Near-IR Dead Cell kit (Invitrogen), CD3-BV785 (Biolegend), CD45-BV510 (Biolegend), CD8-PE-Cy7 (Biolegend), CD4-PE-CF594 (Becton Dickinson), and CD25-PE-Cy7 (Becton Dickinson). Intracellular staining was performed using anti-mouse forkhead box P3 (FoxP3)-AF488 (MF23; BD Pharmingen). Intracellular cell staining for IFNγ and flow cytometry was performed as previously described using IFNγ-BV421 (Biolegend) [26 (link)].

Soares K.C., Rucki A.A., Kim V., Foley K., Solt S., Wolfgang C.L., Jaffee E.M, & Zheng L. (2015). TGF-β blockade depletes T regulatory cells from metastatic pancreatic tumors in a vaccine dependent manner. Oncotarget, 6(40), 43005-43015.

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Murine Lymphocyte Immunophenotyping

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Following the isolation of spleen and liver infiltrating lymphocytes from murine livers and hemispleens, cells were stained with Live Dead Near-IR Dead Cell kit (Invitrogen), CD3-APC (Biolegend), CD3-APC-Cy7 (Biolegend), CD8-PeCy7 (Biolegend), CD4-V500 (BD Horizon), CD25-BV421 (Biolegend), CTLA4-BV421 (Biolegend) and CD69-FITC (BD Pharmingen) for 30 minutes and assayed on an LSR II flow cytometer (BD Biosciences).

Soares K.C., Rucki A.A., Wu A.A., Olino K., Xiao Q., Chai Y., Wamwea A., Bigelow E., Lutz E., Liu L., Yao S., Anders R.A., Laheru D., Wolfgang C.L., Edil B.H., Schulick R.D., Jaffee E.M, & Zheng L. (2015). PD-1/PD-L1 blockade together with vaccine therapy facilitates effector T cell infiltration into pancreatic tumors. Journal of immunotherapy (Hagerstown, Md. : 1997), 38(1), 1-11.

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