Under anesthesia, serums were collected from WKY and SHR and stored at -80°C for analysis. Serum levels of IL-17A and IL-10 were analyzed by using enzyme-linked immunosorbent assay (ELISA) kits: IL-17A (BMS635; Thermo Fisher Scientific, Waltham, MA, USA) and IL-10 (R1000; R&D Systems, Inc., Minneapolis, MN, USA). The optical density value was measured at 450 nm, and the concentrations of IL-17A and IL-10 were calculated with the standard curves.
Il 17a bms635
Manufactured by Thermo Fisher Scientific
Sourced in United States
IL-17A (BMS635) is a laboratory reagent used in research applications. It functions as a recombinant human interleukin-17A protein. Interleukin-17A is a pro-inflammatory cytokine that plays a role in host defense against extracellular pathogens.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using il 17a bms635
1
Serum Cytokine Levels in Animal Model
2
Serum Cytokine Analysis in Rats
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After sacrifice, serum from the rats was collected and stored at −80°C before analysis. Serum IL-17A, IL-10 and CD25 were analyzed using ELISA, which was performed according to the manufacturer's instructions with ELISA kits [IL17A (BMS635; Thermo Fisher Scientific, Waltham, MA, USA), IL-10 (R1000; R&D Systems, Minneapolis, MN, USA), CD25 (E-EL-R0899; Elabscience Biotechnology, Houston, TX, USA)]. The optical density value at 450 nm was measured. The concentrations of IL-17A, IL-10 and CD25 were calculated according to the standard curves.
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