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Cd3 cd28 mabs

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CD3/CD28 mAbs are a pair of monoclonal antibodies that bind to the CD3 and CD28 receptors on the surface of T cells. These antibodies are commonly used in cell culture systems to activate and expand T cells.

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Lab products found in correlation

Lymphocyte separation medium, by Thermo Fisher Scientific (2 mentions) Lipopolysaccharides (lps), by InvivoGen (2 mentions) Vacutainer, by BD (1 mentions) Legendplex, by BioLegend (1 mentions)

Close spelling variants of this product

CD3 mAb

2 protocols using cd3 cd28 mabs

1

Stimulation of Human PBMCs and Cytokine Measurement

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Human PBMCs were isolated from whole blood by using lymphocyte separation medium (Thermo Fisher Scientific, Waltham, MA). The freshly isolated PBMCs were cultured at 4 × 106/ml with GA (Teva Pharmaceutical Industries Ltd., Israel) at 50 µg/ml, LPS (InvivoGen, San Diego, CA) at 5 μg/ml, or CD3/CD28 mAbs (Biolegend, San Diego, CA) at 2 µg/ml for 3 days, and cytokine production was examined by ELISA (Biolegend, San Diego, CA). The remaining cells were frozen and stored in liquid nitrogen. The serum was isolated from the blood by using BD Vacutainer (Becton Dickinson, Franklin Lakes, NJ), and the serum concentration of cytokines was examined by LEGENDplex™ (Biolegend, San Diego, CA).
Ito K., Ito N., Yadav S.K., Suresh S., Lin Y, & Dhib-Jalbut S. (2021). Effect of switching glatiramer acetate formulation from 20 mg daily to 40 mg three times weekly on immune function in multiple sclerosis. Multiple Sclerosis Journal - Experimental, Translational and Clinical, 7(3), 20552173211032323.
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2

Cytokine Production in Isolated PBMCs

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Human PBMCs were isolated from whole blood by using lymphocyte separation medium (Thermo Fisher Scientific, Waltham, MA). The freshly isolated PBMCs were cultured at 2 × 106/ml cell density unstimulated or with LPS (In vivoGen, San Diego, CA) at 5 μg/ml, or CD3/CD28 mAbs (Biolegend, San Diego, CA) at 2 µg/ml for 3 days, and cytokine production was examined by ELISA (Biolegend, San Diego, CA). The remaining cells were frozen and stored in liquid nitrogen.
Yadav S.K., Ito N., Mindur J.E., Kumar H., Youssef M., Suresh S., Kulkarni R., Rosario Y., Balashov K.E., Dhib-Jalbut S, & Ito K. (2022). Fecal Lcn-2 level is a sensitive biological indicator for gut dysbiosis and intestinal inflammation in multiple sclerosis. Frontiers in Immunology, 13, 1015372.
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