Tsq 8000 evo mass spectrometer

For compound identification, mass spectra were generated by TSQ 8000 Evo mass spectrometer coupled to a Thermo Trace GC equipped with a TriPlus RSH Autosampler (Thermo Scientific). The samples (1 µL) were injected on column. The separation of the volatiles was performed on a DB-FFAP capillary column (30 m × 0.25 mm, 0.25 µm film thickness; Agilent) connected with a fused silica pre-column capillary (5 m × 0.53 mm; Handel Müller GmbH) using helium as the carrier gas at a constant flow rate of 2.5 mL/min, by application of the following temperature program: 40 °C for 6 min, then raised to 240 °C at a rate of 7 °C/min and held at 240 °C for 25 min. The injection port was set at 50 °C, and the transfer line to 240 °C with an increase of 14.5 °C/s.
The identification was done in the electron ionization (EI) mode (70 eV). The spectra for identification were recorded in the full scan mode from 35–250 amu with a scan time of 11 ms. The ion source temperature was 220 °C. NIST mass spectral library software (version 2.2; Standard Reference Data Program of the National Institute of Standards and Technology, distributed by Agilent Technologies) was used to assist in compound identification. Most of the compounds identified by the MS-Spectra were verified by commercially available standards (see supporting information S14).