Freezone plus 4

NDH-4338 NSs were prepared using sonoprecipitation, an evaporative-ultrasonication method [37 (link)]. Briefly, NDH 4338 was dissolved in acetone (i.e., the solvent) and injected all at once using a needle (30 G ½) into water (i.e., the antisolvent) containing Vitamin E TPGS, and the resulting mixture was placed in an ice bath. The ultrasonic probe was placed into the antisolvent solution and operated with one minute off between each minute using a Branson Sonifier 450 (Branson Ultrasonics, Danbury, CT, USA) at amplitude settings of 50, 60, and 70% and processing times of 5, 10, and 15 min. During the pause, the probe was removed from the mixture to let it cool. All NSs were lyophilized (FreeZone Plus 4.5, Labconco, Kansas City, MO, USA) with liquid nitrogen for 72 h with vacuum pressure below 20 mTorr and condenser temperature at −80 °C. Samples were reconstituted with refrigerated (2–8 °C) water and gently vortexed.

OG amyloid fibrils aerogel and hydrogel were fabricated from 2 wt% aqueous amyloid fibril dispersion. The used solutions contained either mature rigid amyloid fibrils obtained from the heating process (90 °C), or the mixture of mature rigid fibrils and worm‐like fibrils yielded from the heating process and a successive incubation at RT (25 °C). Before any further processing, the protein solution was dialyzed for 1 day. For aerogel preparation, an aliquot of amyloid fibril dispersion was freeze‐dried (FreeZone Plus 4.5, Labconco, USA) for 2 days in an aluminum cap. For hydrogel synthesis, the amyloid fibril solution was cross‐linked with a NaCl solution at a final concentration of 450 mm and further dialyzed 2 days at 4 °C.