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Cd8 fluorescein isothiocyanate

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CD8-fluorescein isothiocyanate is a fluorescently-labeled antibody that binds to the CD8 surface marker on T cells. It is used for the identification and quantification of CD8-positive cells in flow cytometry applications.

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Lab products found in correlation

Facsdiva software 6, by BD (1 mentions) Lsrfortessa cell analyzer, by BD (1 mentions) Cd4 phycoerythrin pe, by BD (1 mentions) Cd16 cd56 pe, by BD (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Permeabilization solution, by BD (1 mentions) Pe mouse igg1, by BD (1 mentions)

Close spelling variants of this product

Anti-CD8+ [fluorescein isothiocyanate (FITC)] (3 citations) CD8-fluorescein isothiocyanate (FITC), (2 citations) CD8-fluorescein isothiocyanate (clone RPA-T8; (2 citations)

2 protocols using cd8 fluorescein isothiocyanate

1

Multiparameter Cell Surface Phenotyping

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To analyze the cell surface expression of the typical protein markers, adherent cells were incubated with the following anti-human conjugated primary antibodies (BD Biosciences, Franklin Lakes, NJ, USA): CD4-phycoerythrin (PE) (monoclonal, dilution: 0.2 µl/1×106 cells, catalog no.: 565999); CD8-fluorescein isothiocyanate (monoclonal, dilution: 0.2 µl/1×106 cells, catalog no.: 557696); CD56 (monoclonal, dilution: 0.4 µl/1×106 cells, catalog no.: 556647); and HLA-DR-R-PE (monoclonal, dilution: 0.8 µl/1×106 cells, catalog no.: 560943). Unconjugated markers were added to react with anti-mouse PE-conjugated secondary antibody (eBioscience, San Diego, CA, USA; catalog no.: 11-5921; dilution: 1/200). A total of 10,000 labeled cells were analyzed using a BD LSRFortessa™ Cell Analyzer (BD Biosciences, San Jose, CA, USA) and the data obtained were analyzed using BD FACSDiva software 6.0 (BD Biosciences).
Chang Z., Mao G., Sun L., Ao Q., Gu Y, & Liu Y. (2016). Cell therapy for cerebral hemorrhage: Five year follow-up report. Experimental and Therapeutic Medicine, 12(6), 3535-3540.
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2

Immunological Assay for T-Cell Function

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SB-273005 was obtained from GlaxoSmithKline (London, UK); dimethylsulfoxide (DMSO) was purchased from Sigma (St. Louis, MO, USA); cluster of differentiation (CD)3-Per, CD8-fluorescein isothiocyanate, CD8 negative control, interferon (IFN)-γ-phycoerythrin (PE), CD16+CD56-PE, mouse-immunoglobulin G (IgG)1-Per, mouse-IgG1-PE, anti-human IL-4-PE, and permeabilization solution were purchased from BD Biosciences (Franklin Lakes, NJ, USA); and trypsin and culture solutions were purchased from Gibco-BRL (Carlsbad, CA, USA). All other reagents were purchased from Sigma.
WANG S., YANG J., WANG C., YANG Q, & ZHOU X. (2014). SB-273005, an antagonist of αvβ3 integrin, reduces the production of Th2 cells and cytokine IL-10 in pregnant mice. Experimental and Therapeutic Medicine, 7(6), 1677-1682.
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