Log In Sign up
The largest database of trusted experimental protocols

Anti β tubulin 647 antibody

Manufactured by Novus Biologicals
Visit Supplier

The Anti-β-tubulin-647 antibody is a fluorescently labeled antibody that specifically binds to β-tubulin, a component of the cytoskeletal microtubules in cells. This antibody can be used to detect and visualize β-tubulin in various applications, such as immunofluorescence microscopy.

Automatically generated - may contain errors

Lab products found in correlation

Lsm800 confocal microscope, by Zeiss (4 mentions) Zen blue software, by Zeiss (4 mentions) Mouse anti muc5ac, by Thermo Fisher Scientific (2 mentions) Goat anti hpiv3, by Abcam (2 mentions) Anti goat af 568, by Thermo Fisher Scientific (2 mentions) Mouse anti zo 1, by BD (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

β-tubulin (6 citations) Anti-β-Tubulin (2 citations)

2 protocols using anti β tubulin 647 antibody

1

3D Culture Immunofluorescence Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
3D cultures were fixed with 4% paraformaldehyde-PBS for 20 minutes followed by permeabilization with 0.5 % TritonX-100-PBS for 2 hours. Nonspecific protein binding sites were blocked with 5% BSA-PBS for 1 hour. HPIV3 was detected using goat anti-HPIV3 (Abcam) followed by anti-goat AF-568 (Thermo Scientific). Muc5AC was detected using mouse anti-Muc5AC (Thermo Scientific). ZO-1 (tight junctions) was detected using mouse anti-ZO-1 (BD Biosciences; 610966). Anti-mouse-FITC secondary antibody (SantaCruz) was used for both Muc5AC and ZO-1 staining. Anti-β-tubulin-647 antibody (Novus Biologicals) was used for detection of β-tubulin. Membranes were placed on glass slides using a mounting medium supplemented with 0.1 mM 4,6-diamidino-2-phenylindole (DAPI; Molecular Probes), covered with a coverslip, and edges sealed with nail polish. A Zeiss LSM 800 confocal microscope coupled with AiryScan module was used for detection, Zeiss Zen Blue software was employed for image analysis.
Cox R.M., Sourimant J., Toots M., Yoon J.J., Ikegame S., Govindarajan M., Watkinson R.E., Thibault P., Makhsous N., Lin M.J., Marengo J.R., Sticher Z., Kolykhalov A.A., Natchus M.G., Greninger A.L., Lee B, & Plemper R.K. (2020). Orally Efficacious Broad-Spectrum Allosteric Inhibitor of Paramyxovirus Polymerase. Nature microbiology, 5(10), 1232-1246.
+ Open protocol
+ Expand
2

3D Culture Immunofluorescence Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
3D cultures were fixed with 4% paraformaldehyde-PBS for 20 minutes followed by permeabilization with 0.5 % TritonX-100-PBS for 2 hours. Nonspecific protein binding sites were blocked with 5% BSA-PBS for 1 hour. HPIV3 was detected using goat anti-HPIV3 (Abcam) followed by anti-goat AF-568 (Thermo Scientific). Muc5AC was detected using mouse anti-Muc5AC (Thermo Scientific). ZO-1 (tight junctions) was detected using mouse anti-ZO-1 (BD Biosciences; 610966). Anti-mouse-FITC secondary antibody (SantaCruz) was used for both Muc5AC and ZO-1 staining. Anti-β-tubulin-647 antibody (Novus Biologicals) was used for detection of β-tubulin. Membranes were placed on glass slides using a mounting medium supplemented with 0.1 mM 4,6-diamidino-2-phenylindole (DAPI; Molecular Probes), covered with a coverslip, and edges sealed with nail polish. A Zeiss LSM 800 confocal microscope coupled with AiryScan module was used for detection, Zeiss Zen Blue software was employed for image analysis.
Cox R.M., Sourimant J., Toots M., Yoon J.J., Ikegame S., Govindarajan M., Watkinson R.E., Thibault P., Makhsous N., Lin M.J., Marengo J.R., Sticher Z., Kolykhalov A.A., Natchus M.G., Greninger A.L., Lee B, & Plemper R.K. (2020). Orally Efficacious Broad-Spectrum Allosteric Inhibitor of Paramyxovirus Polymerase. Nature microbiology, 5(10), 1232-1246.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!