Lc ms grade water

Manufactured by Biosolve

Sourced in France, United States, Germany

LC-MS grade water is a highly purified form of water specifically designed for use in liquid chromatography-mass spectrometry (LC-MS) applications. It is characterized by its low level of impurities and high degree of purity, making it suitable for sensitive analytical techniques.

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MS-grade water (4 citations) LC-MS grade (4 citations)

14 protocols using lc ms grade water

Characterization of Biomolecular Interactions

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Cytochrome c (12.4 kDa)
from equine heart, myoglobin (17.6 kDa) from equine heart, pepsin
(35 kDa) from porcine gastric mucosa, bovine serum albumin (BSA, 66.4
kDa), conalbumin (77 kDa) from chicken egg white, concanavalin A (102
kDa) from Canavalia ensiformis, immunoglobulin
G (IgG, ∼150 kDa) from human serum, beta amylase (β-amylase,
223.8 kDa) from sweet potato, chaperonin 60 (GroEL, ∼800 kDa)
from Escherichia coli, ammonium acetate,
tris-acetate, potassium chloride, ethylenediaminetetraacetic acid
(EDTA), adenosine-5′-triphosphate (ATP), magnesium chloride,
ammonium hydroxide, and acetic acid were all purchased from Sigma-Aldrich
(Zwijndrecht, The Netherlands). Methanol, acetone, and LC-MS grade
water were purchased from Biosolve (Valkenswaard, The Netherlands).
Nanospray needles were homemade from preheated borosilicate glass
capillaries (Science Products GmbH, Hofheim, Germany) on a DMZ universal
electrode puller (Zeitz-Instruments Vertriebs GmbH, Munich, Germany)
followed by gold coating with a SC7640 sputter coater (Quorum Technologies,
Kent, UK).

Mathew A., Buijs R., Eijkel G.B., Giskes F., Dyachenko A., van der Horst J., Byelov D., Spaanderman D.J., Heck A.J., Porta Siegel T., Ellis S.R, & Heeren R.M. (2021). Ion Imaging of Native Protein Complexes Using Orthogonal Time-of-Flight Mass Spectrometry and a Timepix Detector. Journal of the American Society for Mass Spectrometry, 32(2), 569-580.

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Optimized Protein Denaturation and Digestion

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Denatured samples were applied to pre-wet Vivacon Hydrosart^® stabilized cellulose filters (30KDa molecular weight cutoff; Sartorius) connected to LoBind tubes for reduction and alkylation steps. Samples were reduced with 20 mM dithiothreitol (DTT; Sigma) in 8 M Urea buffer for 30 min at 37 °C, followed by alkylation of free cysteines with 50 mM iodoacetamide (IAA; Sigma) in 8 M urea buffer for 20 min at room temperature (RT) in the dark. The flowthrough was discarded, and filters were washed three times with 2 M urea in 50 mM ammonium acetate, pH 5.5 (2 M urea buffer) before digestion for 3 h at 37°C using rLys-C and trypsin from the AccuMAP^TM Low pH Protein Digestion Kit (Promega). Tubes were subsequently centrifuged to collect tryptic peptides in the flowthrough. 20% acetonitrile (Biosolve) in 50 mM ammonium acetate pH 5.5 was added to the filter to reduce non-specific binding followed by a further centrifugation step. Finally, 1% acetic acid in LC-MS grade water (Biosolve) was added to the flowthrough to inactivate enzymes prior to storage of the sample at −80 °C until solid-phase extraction and analysis by LC-MS/MS.

Tang C., Verwilligen A., Sadoff J., Brandenburg B., Sneekes-Vriese E., van den Kerkhof T., Dillen L., Rutten L., Juraszek J., Callewaert K., Janssen S., Huizingh J., Euler Z., Schilperoord T., Verhemeldonck M., Langedijk J.P., Hendriks J, & Stieh D.J. (2024). Absolute quantitation of binding antibodies from clinical samples. NPJ Vaccines, 9, 8.

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Protein Extraction and Mass Spectrometry

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Ubiquitin (8.6 kDa) from bovine erythrocytes,
concanavalin A (102 kDa) from Canavalia ensiformis, and ammonium acetate were all purchased from Sigma-Aldrich (Zwijndrecht,
The Netherlands). Cesium iodide (CsI; 392.7 to 11 304 Da) was
obtained from Thermo Fisher Scientific, The Netherlands. Methanol,
isopropanol, and LC–MS grade water were purchased from Biosolve
(Valkenswaard, The Netherlands).

Mathew A., Giskes F., Lekkas A., Greisch J.F., Eijkel G.B., Anthony I.G., Fort K., Heck A.J., Papanastasiou D., Makarov A.A., Ellis S.R, & Heeren R.M. (2023). An Orbitrap/Time-of-Flight Mass Spectrometer for Photofragment Ion Imaging and High-Resolution Mass Analysis of Native Macromolecular Assemblies. Journal of the American Society for Mass Spectrometry, 34(7), 1359-1371.

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Decitabine Formulation Development

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Decitabine was purchased from LC Laboratories^® (Woburn, MA, USA). Labrafac^® WL1349 (caprylic-capric acid triglycerides), Labrafil^® M1944CS (oleoyl macrogol-6 glycerides), Peceol^® (glycerol mono-oleates), and Transcutol^® HP (THP) (highly purified diethylene glycol monoethyl ether) were kindly provided by Gattefossé (Saint-Priest, France). Lipoïd^® S75-3 (soybean lecithin: 69% phosphatidylcholine and 10% phosphatidylethanolamine) and Kolliphor^® HS15 (mixture of free polyethylene glycol [PEG] 660 and PEG 660 hydroxystearate) were kindly supplied by Lipoïd^® and BASF, respectively (Ludwigshafen, Germany). Deionized water was obtained from a Milli-Q plus system (Merck-Millipore, Darmstadt, Germany). Sodium chloride and disodium hydrogen phosphate were purchased from Prolabo (Fontenay-Sous-Bois, France) and sucrose was purchased from Merck-Millipore. Sodium phosphate dibasic dodecahydrate, potassium dihydrogen phosphate, formic acid, ammonium acetate, Triton^® X-100, Tween^® 80, Span^® 80, and formic acid were obtained from Sigma-Aldrich (St Quentin-Fallavier, France). Captex^® 8000 (glyceryl tricaprylate) was a gift from Abitec Corp. (Saint-Quentin Fallavier, France).
Absolute ethanol, methanol ULC/MS grade, acetonitrile, and LC-MS grade water were purchased from Biosolve (Dieuze, France). Acetone was purchased from VWR (Fontenay-sous-Bois, France).

Briot T., Roger E., Lautram N., Verger A., Clavreul A, & Lagarce F. (2017). Development and in vitro evaluations of new decitabine nanocarriers for the treatment of acute myeloid leukemia. International Journal of Nanomedicine, 12, 8427-8442.

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Metabolite and Lipid Profiling Protocol

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LC-MS grade water, methanol, isopropanol, and acetonitrile were purchased from BioSolve (Valkenswaard, The Netherlands). The MS grade mobile phase additives; ammonium formate, ammonium acetate, ammonium hydroxide, and formic acid were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). The list of all standards along with their vendor information is available online http://curatr.mcf.embl.de/inventory/. The Mass Spectrometry Metabolite Library of Standards (MSMLS) kit was purchased from IROA Technologies (MA, USA). All other metabolites and lipid standards were purchased from Sigma-Aldrich, Avanti Polar Lipids, and Matreya. HCT-116 (ATCC® CCL-247) cells were culture to 80% confluency using vendor provided protocol (ATCC; VA, USA).

Phapale P., Palmer A., Gathungu R.M., Kale D.D., Brügger B., & Alexandrov T. (2020). Public LC-Orbitrap-MS/MS Spectral Library for Metabolite Identification.

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Metabolite and Lipid Profiling Protocol

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Phapale P., Palmer A., Gathungu R.M., Kale D., Brügger B, & Alexandrov T. (2021). Public LC-Orbitrap Tandem Mass Spectral Library for Metabolite Identification. Journal of proteome research, 20(4).

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Peptide Synthesis with HBTU and Fmoc-Protected Amino Acids

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2-(1H-Benzotriazol-1-yl)-1,1,3,3-tetramethyluronium fluorophosphate (HBTU) and N-Fmoc protected amino acids were obtained from Iris Biotech GmbH. 4-Mercaptophenylacetic acid (97%, MPAA), 3-mercaptopropionic acid (MPA), tris(2-carboxyethyl)phosphine hydrochloride (≥98%, TCEP), thiophenol, triisopropylsilane (TIS), guanidine hydrochloride (≥99%), sodium phosphate dibasic dihydrate (≥99%), hydrochloric acid (reagent grade, 37%), and sodium hydroxide (pellets, 97%) were purchased from Sigma-Aldrich. All other reagents were purchased from Acros Organics or Merck and were of the purest grade available. Peptide synthesis grade N,N-dimethylformamide, dichloromethane, diethylether, acetonitrile, heptane, LC–MS-grade acetonitrile (0.1% TFA), LC–MS-grade water (0.1% TFA), N,N-diisopropylethylamine (DIEA), and acetic anhydride were purchased from Biosolve and Fisher-Chemical. Trifluoroacetic acid (TFA) was obtained from Biosolve. Decane (synthesis grade) was purchased from Merck. Solvents and reagents were used as received. Water was purified with a Milli-Q UltraPure Water Purification System.

Ollivier N., Toupy T., Hartkoorn R.C., Desmet R., Monbaliu J.C, & Melnyk O. (2018). Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides. Nature Communications, 9, 2847.

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Analytical Protocol for Dental Materials

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Alpha amylase, bisphenol A-d16, Celite 545, deuterium, diethyl phthalate-3,4,5,6-d4, acetic acid (LC-MS grade), formic acid (LC-MS grade), ammonium acetate (LC-MS ultra), ammonium formate (LC-MS), methanol (LC-MS grade), mucin from porcine stomach, potassium thiocyanate, sodium phosphate monobasic dehydrate and uric acid were purchased from Sigma-Aldrich (Diegem, Belgium). LC-MS-grade water was obtained from Biosolve (Valkenswaard, The Netherlands). Ethanol absolute and sodium hydroxide were purchased from Fisher Scientific (Aalst, Belgium). Potassium chloride and sodium chloride were obtained from VWR (Haasrode, Belgium). Urea was purchased from GE Healthcare Europe GmbH (Diegem, Belgium). Palladium on activated charcoal was obtained This article is protected by copyright. All rights reserved.
from Acros Organics (Geel, Belgium). Clearfil SE Bond was obtained from Kuraray Noritake Dental Inc (Okayama, Japan). Tetric EvoFlow was obtained from Ivoclar Vivadent (Schaan, Liechtenstein).

Putzeys E., Cokic S.M., Chong H., Smet M., Vanoirbeek J., Godderis L., Van Meerbeek B., Van Landuyt K.L, & Duca R.C. (2017). Simultaneous analysis of bisphenol A based compounds and other monomers leaching from resin-based dental materials by UHPLC-MS/MS. Journal of separation science, 40(5).

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Enzymatic Saccharification of Rice Straw

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All the chemicals used in this study were procured from Sigma Aldrich and Merck, India.
¹³C gluconic acid (99 atom % ¹³C) were purchased from Omicron Biochemicals, USA, and LC/MS grade water was obtained from Biosolve Chimie, SARL, France. Commercial enzymes Cellulase from T. reesei ATCC 26921 (Celluclast 1.5L) and β-glucosidase (Novozymes 188) were procured from Sigma, Aldrich India. Cellic^® Ctec2 and Cellic^® HTec3 were a kind gift from Novozymes, Mumbai, India. Avicel^® PH 101 and dilute acid-treated rice (Oryza sativa) straw were used as substrates for enzymatic saccharification studies. Rice (Oryza sativa) straw was procured from the local market at Mathura (27.28° N, 77.41° E) in Uttar Pradesh (North, India) air-dried, ground to the particle size of ~ 10 mm using a knife mill ,and stored in an air tight container for further use. All the experiments were conducted from the single stock of harvested rice straw.

Dixit P., Basu B., Puri M., Tuli D.K., Mathur A.S, & Barrow C.J. (2019). A screening approach for assessing lytic polysaccharide monooxygenase activity in fungal strains. Biotechnology for Biofuels, 12, 185.

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Solid-Phase Peptide Synthesis Reagents

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1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate (HATU), 2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium fluorophosphate (HBTU) and N-Fmoc-protected amino acids were obtained from Iris Biotech GmbH. Piperidine, diisopropylcarbodiimide (DIC), N-hydroxybenzotriazole (HOBt), MPAA (97%, MPAA), 3-mercaptopropionic acid (MPA), tris(2-carboxyethyl)phosphine hydrochloride (≥98%, TCEP·HCl), thiophenol, triisopropylsilane (TIS), guanidine hydrochloride (≥99%, Gn·HCl), hydrochloric acid (reagent grade, 37%), and sodium hydroxide (pellets, 97%) as well as other reagents were purchased from Sigma Aldrich, Acros Organics or Merck and were of the purest grade available. Peptide synthesis grade N,N-dimethylformamide (DMF), dichloromethane, diethyl ether, acetonitrile, heptane, LC-MS-grade acetonitrile (0.1% TFA), LC-MS grade water (0.1% TFA), N,N-diisopropylethylamine (DIEA), acetic anhydride (Ac₂O), and trifluoroacetic acid (TFA) were purchased from Biosolve and Fisher-Chemical. NovaSyn TGR solid support was purchased from Sigma Aldrich. Bis(2-sulfenylethyl)amino trityl 1% divinylbenzene cross-linked polystyrene solid support (SEA PS) was produced on demand by Roowin. Solvents and reagents were used as received.

Diemer V., Ollivier N., Leclercq B., Drobecq H., Vicogne J., Agouridas V, & Melnyk O. (2020). A cysteine selenosulfide redox switch for protein chemical synthesis. Nature Communications, 11, 2558.

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