The plasma levels for BUN and creatinine were measured by a Fujidrychem7000 and drychem slide system (FUJIFILM, Kanagawa, Japan) following the manufacturer's protocol.
Drychem slide system
Manufactured by Fujifilm
Sourced in Japan
The Drychem slide system is a laboratory equipment product by Fujifilm. It is designed for the analysis and examination of samples on slides. The core function of the Drychem slide system is to provide a reliable and automated platform for slide-based diagnostics and testing.
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2 protocols using drychem slide system
1
Measuring Plasma BUN and Creatinine Levels
2
Measurement of Serum Biomarkers in Chronic Kidney Disease
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Serum concentration of BUN and creatinine was measured using FUJI DRI‐CHEM 7000 and a dry chem slide system (FUJIFILM, Kanagawa, Japan) as per the protocol described by the manufacturer. The serum apelin and elabela concentration was determined via enzyme‐linked immunoassays using an EIA Kit (Phoenix Pharmaceuticals, Inc., Burlingame, CA, USA) as per the protocol described by the manufacturer. The serum concentration of IS was measured via HPLC, as described previously, with a few modifications.15 The HPLC system consisted of a CBM‐20A communication bus module, SPD‐20A, a degasser and an LC‐20 AD pump (SHIMADZU Co., Kyoto, Japan). The conditions for HPLC were as follows: column, Unison UK‐C18 (3 μm, 250 mm × 4.6 mm).
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