Waters 2478 dual λ absorbance detector

The total ginsenoside content and ginsenoside composition of UGBE and GBE were analyzed using a Waters 1525 binary high-performance liquid chromatography (HPLC) system (Milford, US). The separation of UGBE was performed on an Eurospher analytical column (100-5 C18, 250 mm × 3.0 mm, 5 μm, Knauer, Berlin, DE) by gradient elution at room temperature. The eluent was a mixture of A (acetonitrile for HPLC) and B (distilled water for HPLC). The process of elution was carried out according to the following conditions: 0 mins, 17% of A; 25 mins, 25% of A; 50 mins, 40% of A; 105 mins, 60% of A; 110 mins, 100% of A. The flow rate was 0.8 mL/min, the injection volume was 20 μL, and the chromatograms were obtained using a Ultraviolet-visible Waters 2478 Dual λ Absorbance Detector (Milford, USA) at 203 nm.

UGBE and GBE water solvent extracts were kindly provided by Professor Sung Kwon Ko of Semyung University. We analyzed the constituents of UGBE and GBE bythe Waters 1525 binary HPLC system (Waters, Milford, MA, USA). Separation of UGBE was performed on an analytical column (Eurospher, 100-5 C18, 250 mm × 3.0 mm, 5μm; Knauer, Berlin, Germany) by gradient elution at room temperature. The eluent was a mixture of acetonitrile for HPLC (A) and distilled water for HPLC (B). The elution process was performed according to the following conditions: 0 min, 17% of A; 25 min, 25% of A; 50 min, 40% of A; 105 min, 60% of A; 110 min, 100% of A. The flow rate was 0.8 mL/min, injection volume was 20μL, and chromatograms were acquired by a UV/VIS Waters 2478 Dual λ Absorbance Detector (Waters) at 203 nm. After 10 h of ultrasonification, ginsenosides Rb1, Rb2, Rd, Re, Rf, Rg1, Rg2, Rg3, Rg6, Rh1, Rh4, Rk1, Rk3, F1, and F4 were identified. UGBE solution was orally administered to the rats once/d at doses of 100 mg/kg body weight (b.w.), 250 mg/kg b.w., and 500mg/kg b.w.