Yeast extract powder 1, industrial Yeast extract powder 2 and peptone 1 were purchased from Angie Yeast Co., Ltd. (China). Soy meal 1 was purchased from Shandong Longkete Enzyme Preparations Co., Ltd. (China). Soy peptone and soy meal 2 were purchased from Xi Wang Co., Ltd. (China). Yeast extract, peptone, industrial peptone, fish meal peptone, beef extract fermentation, cottonseed meal, bone peptone, tryptone, beef meal, corn steep powder, Yeast extract fermentation and beef peptone were purchased from Sinopharm Chemical Reagent Co., Ltd. (China).
Tryptone
Manufactured by Sinopharm
Sourced in China
Tryptone is a complex mixture of peptides and amino acids derived from the enzymatic digestion of casein. It serves as a nutrient source for the growth of various microorganisms in microbiology and biotechnology applications.
Automatically generated - may contain errors
Lab products found in correlation
Yeast extract, by Sinopharm (8 mentions)
Sodium chloride (nacl), by Sinopharm (6 mentions)
Peptone, by Sinopharm (4 mentions)
Agar powder, by Sinopharm (2 mentions)
Mgso4 7h2o, by Sinopharm (2 mentions)
Dopamine hydrochloride, by Merck Group (2 mentions)
Fish meal peptone, by Sinopharm (1 mentions)
Corn steep powder, by Sinopharm (1 mentions)
Yeast extract, by Maclin Biochemical Technology (1 mentions)
Milli q, by Merck Group (1 mentions)
Calcium sulfate, by Sinopharm (1 mentions)
Glycerin, by Sinopharm (1 mentions)
Maltose, by Sinopharm (1 mentions)
Sorbitol, by Sinopharm (1 mentions)
Kanamycin, by Sinopharm (1 mentions)
Ampicillin, by Sinopharm (1 mentions)
Normal saline, by Sinopharm (1 mentions)
Phosphate buffered saline (pbs), by Sinopharm (1 mentions)
K2hpo4 3h2o, by Sinopharm (1 mentions)
Acetic acid, by Sinopharm (1 mentions)
12 protocols using tryptone
1
Sourcing of Microbial Growth Supplements
2
Conserving and Culturing E. coli ATCC25922
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Escherichia coli ATCC25922 is conserved by the Key Laboratory of Dairy Biotechnology and Safety Control, Yangzhou University (Yangzhou, China). Before use, E. coli cultures were stored in a 1:1 ratio with 70% (v/v) glycerol solution in a −40 °C refrigerator. E. coli was cultured in Luria–Bertani (LB) broth containing 0.5% yeast extract (Macklin Biochemical Co., Ltd., Shanghai, China), 1.0% tryptone (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China), and 1.0% NaCl (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China). The organic acids (AA, LA, BA, CA, and MA) were purchased from Macklin.
Configuration of organic acid solutions: Organic acid solutions of different concentrations for treating E. coli are shown inTable 1 . Based on our previous research [28 (link)], the minimum inhibitory concentration (MIC) was obtained by the microdilution method, and the optimal inhibitory concentration was obtained by the checkerboard method.
Configuration of organic acid solutions: Organic acid solutions of different concentrations for treating E. coli are shown in
3
Synthesis of 1,3-diphenylisobenzofuran
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Valine (99%), triethylamine (99%) and 1,3-diphenylisobenzofuran (DPBF) were purchased from Aladdin Reagent Co., Ltd. (Shanghai, China). Sodium chloride, yeast extract, agar powder, tryptone were purchased from Sinopharm Chemical Reagent Co., Ltd. All chemicals were used directly without further purification. All of solutions were prepared with deionized water from a Millipore water purification system (Milli-Q, Millipore, Billerica, USA).
4
Diverse Biological Reagents Acquisition
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Neutral protease, papain, chitosan, bovine insulin, myoglobin, and tryptophan were purchased from Aladdin Reagent Co., Ltd. (Shanghai, China). Tryptone, yeast extract, Agar, NaCl, glycerin, K2HPO4·3H2O (AR), sorbitol, MgSO4·7H2O (AR), trisodium citrate, MgCl2 (AR), kanamycin, ampicillin, peptone, maltose, sodium acetate, calcium sulfate, acetic acid, Triton X-100, phosphate-buffered saline, normal saline, trichloroacetic acid, and other reagents (AR) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
5
Cultivation of Ganoderma lucidum Strain CGMCC5.26
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Ganoderma lucidum CGMCC5.26 was purchased from China General Microbiological Culture Collection Center (CGMCC) and maintained on potato dextrose agar (PDA) slants at 4 °C. In order to get consistent metabolites and transcriptome data, a semi‐synthetic media was selected in this study. The medium was composed of glucose 20 g l−1, yeast nitrogen base without amino acids (YNB) 5 g l−1, tryptone 5 g l−1, KH2PO4 4.5 g l−1 and MgSO4·7H2O 2 g l−1 at initial pH. Glucose, KH2PO4 and MgSO4·7H2O purchased from Sinopharm (Beijing, China), YNB purchased from BioDee Biotechnology Co., Ltd (Beijing, China), and tryptone purchased from OXOID (Hants, UK). The medium has been used many years to study the secondary metabolism of G. lucidium (Peng et al.,2015 ; Ma et al., 2018 , 2019 ). The seed was in a 250‐ml flask containing 80 ml medium and kept at 30 °C on a rotary shaker (150 rpm) for ten days.
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Ganoderma lucidum CGMCC5.26 was purchased from China General Microbiological Culture Collection Center (CGMCC) and maintained on potato dextrose agar (PDA) slants at 4 °C. In order to get consistent metabolites and transcriptome data, a semi‐synthetic media was selected in this study. The medium was composed of glucose 20 g l−1, yeast nitrogen base without amino acids (YNB) 5 g l−1, tryptone 5 g l−1, KH2PO4 4.5 g l−1 and MgSO4·7H2O 2 g l−1 at initial pH. Glucose, KH2PO4 and MgSO4·7H2O purchased from Sinopharm (Beijing, China), YNB purchased from BioDee Biotechnology Co., Ltd (Beijing, China), and tryptone purchased from OXOID (Hants, UK). The medium has been used many years to study the secondary metabolism of G. lucidium (Peng et al.,
6
Tetracycline Antibiotic Soil Extraction
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All chemicals were purchased from Sinopharm Chemical Reagent Co. Ltd., including tetracycline standard, tryptone, yeast extract, NaCl, glucose, chromatography grade methanol and acetonitrile, analytical grade sodium dihydrogen phosphate, oxalic acid, disodium ethylenediaminetetraacetate, and citric acid.
The soil sample was obtained from the sludge in the sediment pool of a pig factory using tetracycline antibiotics as feed additive for a long-term (4 a) in Changsha. The average temperature and pH value of the sampling location in this study was 20°C and about 6.8. The latitude and longitude of our sampling locations are 28.1932 and 112.6973, respectively.
Lysogeny broth (LB) medium contained the following (in g L−1): tryptone, 10; yeast extract, 5; NaCl, 5; glucose, 10. The pH of LB medium was adjusted to 7.0–7.2 before autoclaving at 121°C for 20 min.
The soil sample was obtained from the sludge in the sediment pool of a pig factory using tetracycline antibiotics as feed additive for a long-term (4 a) in Changsha. The average temperature and pH value of the sampling location in this study was 20°C and about 6.8. The latitude and longitude of our sampling locations are 28.1932 and 112.6973, respectively.
Lysogeny broth (LB) medium contained the following (in g L−1): tryptone, 10; yeast extract, 5; NaCl, 5; glucose, 10. The pH of LB medium was adjusted to 7.0–7.2 before autoclaving at 121°C for 20 min.
7
Antarctic Psychrobacter sp. ANT206 Cultivation
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The strains used in this study are listed in Table 7 .
Psychrobacter sp. ANT 206 (GenBank accession numbers MK968312), isolated from a sample of Antarctic sea-ice (68° 30’ E, 65° 00’ S), was cultured in 2216E sea water medium (5 g of peptone (Sinopharm, Beijing, China) and 1 g of yeast extract powder (Sinopharm, Beijing, China) per 1 L of solution, pH 7.5) at 12 °C with shaking at 200 rpm and then frozen in glycerol at −80 °C for long term storage. Psychrobacter sp. ANT206 was used as a source of chromosomal DNA. All strains were stored in 16% glycerol at −20 °C, BL21/pET-28a(+) and E. coli BL21 were cultured in Luria Bertani (LB) medium (10 g of tryptone (Sinopharm, Beijing, China), 5 g of yeast extract powder, and 10 g of NaCl (Sinopharm, Beijing, China) per 1 L of solution, pH 7.0) at 37 °C.
Psychrobacter sp. ANT 206 (GenBank accession numbers MK968312), isolated from a sample of Antarctic sea-ice (68° 30’ E, 65° 00’ S), was cultured in 2216E sea water medium (5 g of peptone (Sinopharm, Beijing, China) and 1 g of yeast extract powder (Sinopharm, Beijing, China) per 1 L of solution, pH 7.5) at 12 °C with shaking at 200 rpm and then frozen in glycerol at −80 °C for long term storage. Psychrobacter sp. ANT206 was used as a source of chromosomal DNA. All strains were stored in 16% glycerol at −20 °C, BL21/pET-28a(+) and E. coli BL21 were cultured in Luria Bertani (LB) medium (10 g of tryptone (Sinopharm, Beijing, China), 5 g of yeast extract powder, and 10 g of NaCl (Sinopharm, Beijing, China) per 1 L of solution, pH 7.0) at 37 °C.
8
Photosensitizer-mediated Antimicrobial Protocol
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Chlorin e6 (Ce6) was purchased from Frontier Scientific Inc. (West Logan, UT, USA). 1,3-diphenylisobenzofuran (DPBF) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Perfluorodecalin (FDC), lecithin, and cholesterol were purchased from Aladdin (Shanghai, China). Yeast extract, tryptone, agar, and crystal violet (CV) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Distilled water (DW) used in the experiments was made from a Milli-Q Direct 16 Water Purification System (Millipore Corporation, Bedford, MA, USA) with resistivity higher than 18.2 MΩ cm−1. Other chemicals were supplied by Sinopharm Chemical Reagent Co., Ltd., China, and used as received. E. coli was purchased from Guangdong Microbial Culture Collection Center (Guangzhou, China) and A. baumannii was kindly supplied by Dr. Yishan Zheng at The Second Hospital of Nanjing.
9
Antimicrobial Efficacy and Cytotoxicity Evaluation
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Dopamine hydrochloride was purchased from Sigma-Aldrich. Tri(hydroxymethyl) amino methane (Tris)-HCl, tryptone, yeast extract, agar powder and other chemicals of at least analytical reagent were obtained from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). S. aureus (ATCC 29213) and E. coli (ATCC 8739) were obtained from China General Microbiological Culture Collection Center (Beijing, China). Human cervix carcinoma cells (HeLa) were obtained from China Infrastructure of Cell Line Resources (Beijing, China). Aqueous solutions were prepared using deionized water (Milli-Q system).
10
Purification and Analysis of Enzymes
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NaCl, yeast extract, Tryptone, agar, imidazole, d‐ glucose, Nicotinamide adenine dinucleotide (NAD+), 3,5‐dinitrosalicylic acid (DNS), sodium hydroxide, potassium sodium tartrate, phenol, sodium sulfite, and sodium nitrate hexahydrate were provided by Sinopharm (Shanghai, China). Kanamycin, isopropyl‐β‐d‐ thiogalactoside (IPTG), and dopamine hydrochloride were all purchased from Sigma (St. Louis, MO, USA). The Ni‐NTA resin was supplied by Nano‐Micro Co. Ltd (Suzhou, China). Unless otherwise stated, all chemicals and reagents were commercial analytical‐ or biological‐grade.
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