Pyridine

High-fructose corn syrup (F55) was supplied by Guangzhou Shuangqiao Co., Ltd. (Guanzhou, China). Nonadecanoic acid was purchased from Sigma-Aldrich (Sigma, San Diego, USA). Methanol, hexane, and chloroform were of HPLC (High Performance Liquid Chromatography) grade and were from Merck (Darmstadt, German). Pyridine, O-methylhydroxylamine hydrochloride, N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA), and trimethylchlorosilane (TMCS) were from J&K Scientific Co. Ltd. (Tianjing, China).

Chemical standards of sterol lipids were purchased from J&K Scientific (Shanghai, China), Avanti Polar Lipids (Alabaster, USA), Steraloids (Newport, RI, USA), and Sigma-Aldrich (St. Louis, MO, USA). Acetic acid was purchased from Fisher Scientific (Morris Plains, NJ, USA). Picolinic acid, pyridine, and 2-methyl-6-nitrobenzoic anhydride (MNBA) were purchased from J&K Scientific (Shanghai, China). 4-dimethylaminopyridine (DMAP) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Triethylamine (TEA) was purchased from China National Pharmaceutical Group Corporation (Shanghai, China). Potassium hydroxide was purchased from BBI Life Science Corporation (Shanghai, China). Butylated hydroxytoluene (BHT) was purchased from Sigma-Aldrich (St. Louis, MO, USA). LC-MS grade water (H₂O), acetonitrile (ACN), methanol (MeOH), HPLC grade dichloromethane (DCM), and HPLC grade hexane were purchased from Honeywell (Muskegon, MI, USA).

Branched polyethyleneimine (PEI, Mw 2 kDa), 2-(N,N-diethylamino)ethyl methacrylate (DEA), Cysteamine (95%), Azobisisobutyronitrile (AIBN), Succinic anhydride (SA), cis-Aconitic anhydride, dimethyl sulfoxide (DMSO), Triethylamine (TEA), pyridine, and acetone were purchased from J&K Scientific Ltd. (Beijing, China). ZrOCl2∙8H2O, Tetrakis (4-carboxyphenyl)porphyrin (TCPP), N,N-Dimethylformamide (DMF), N-ethyl-N-(3-(dimethylamino)-propyl) carbodiimide hydrochloride (EDC), and N-hydroxysuccinimide (NHS) were supplied by Aladdin Industrial Co., Ltd. (Shanghai, China). The siZEB1 (GTT​GTT​CTG​CCA​ACA​GTT​G) and siZEB2 (GGC​CGA​ATG​AGA​AAC​AAT​A) target both human and mice’s genes, and Cy5-labeled siZEB1/2 were designed and synthesized by RiboBio (Guangzhou, China). Lipofectamine 2000 was purchased from Invitrogen (Carlsbad, United States), and the LPS was obtained from Sigma-Aldrich (Missouri, United States). The Cell Counting Kit-8 (CCK-8) was bought from Boster Biological Technology (Wuhan, China). DAPI and total RNA Reagent Trizol were purchased from Life Technologies (New York, United States). The PrimeScript®RT Reagent Kit with gDNA Eraser was provided by TaKaRa (Beijing, China). Quantitative real-time qPCR kits (iTaq™ universal SYBR^® Green Supermix) were purchased from Bio-Rad (California, United States).