6545xt advancebio lc q tof

Manufactured by Agilent Technologies

The 6545XT AdvanceBio LC/Q-TOF is a high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (LC/Q-TOF) system designed for advanced biomolecular analysis. It provides accurate mass measurement and high-resolution separation capabilities.

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Lab products found in correlation

1290 infinity 2 hplc system, by Agilent Technologies (1 mentions) Vertex 80 ftir spectrometer, by Bruker (1 mentions) Hyperion 3000 microscope, by Bruker (1 mentions) Nexsa base model, by Thermo Fisher Scientific (1 mentions) X pert pro mpd, by Philips (1 mentions) Masshunter bioconfirm software, by Agilent Technologies (1 mentions)

Close spelling variants of this product

6545XT AdvanceBio Q-TOF 6545XT Q-TOF

3 protocols using 6545xt advancebio lc q tof

Agilent 6545XT AdvanceBio LC/Q-TOF Peptide Analysis

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The reported spectra
were acquired on an Agilent 6545XT AdvanceBio LC/Q-TOF equipped with
an e-MSion ExD cell for ECD fragmentation. Ionization used the standard
Jet Stream and an Agilent 1290 Infinity II HPLC system for chromatographic
separations. Details of the sample preparation are described in Supporting Information.

Beckman J.S., Voinov V.G., Hare M., Sturgeon D., Vasil’ev Y., Oppenheimer D., Shaw J.B., Wu S., Glaskin R., Klein C., Schwarzer C, & Stafford G. (2021). Improved Protein and PTM Characterization with a Practical Electron-Based Fragmentation on Q-TOF Instruments. Journal of the American Society for Mass Spectrometry, 32(8), 2081-2091.

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Advanced Characterization of Catalytic Materials

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A Bruker 3000 Hyperion Microscope outfitted with a Vertex 80 FTIR spectrometer was utilized on the KBr pellets. The Phillips X’pert Pro MPD (multipurpose diffractometer) was used to perform powder XRD at a scan speed of 2°/min utilizing Cu K radiation (2 = 10–90). Jeol 6390LA/OXFORD XMX N is used for SEM-EDS analysis with accelerating voltage of 0.5 to 30 kV and magnification up to 30k. EDS has a resolution of 136 eV and an area detector of 30 mm². HRTEM was investigated by Jeol/JEM 2100 (200 KV), consisting LaB6 electron gun having lattice resolution and point resolution is 0.14 nm and 0.23 nm, respectively. The free radical test has done by the ESR technique (JES-FA200). The VSM lakeshore model (7400 series) determines the magnetic properties of the catalyst. Nova Station B was used in the N₂ atmosphere for N₂ adsorption-desorption. Before that, the sample was degassed at 80 °C. XPS analysis was obtained from the Nexsa base model made by Thermo Fischer Scientific. FT-RAMAN spectrometer analysis is obtained using Bruker RFS with a wavelength of 50–5000 cm^–1. HPLC with LCMS received from Agilent 6545XT AdvanceBio LC/Q-TOF to know the end products and understand the formation of the iron cluster. The dye concentration was measured by a Genesys 10S UV-Vis spectrophotometer.

Yadav G., Mishra S.R., Gadore V., Yadav N, & Ahmaruzzaman M. (2023). A smart and sustainable pathway for abatement of single and binary mixtures of dyes through magnetically retrievable Ca4Fe9O17 anchored on Biochar matrix. Scientific Reports, 13, 12940.

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Redox Modification Analysis of Yeast PDI

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Recombinant yPDI proteins(20 μg/mL) were reduced with or without 100 μM TCEP in 250 mM sodium acetate buffer, pH 6.5, at 37 °C for 30 min. Samples were treated with 100 μM sodium disulfide (Na₂S₂) at 37 °C for 15 min and were then alkylated with 1 mM iodoacetamide (37 °C, 30 min). Samples were digested with Trypsin Gold (2 μg/mL) in 0.025 % ProteaseMAX Surfactant (37 °C, 3 h) and subjected to LC-ESI-quadrupole time-of-flight MS/MS (LC-ESI-Q-TOF MS/MS). LC-ESI-Q-TOF MS/MS analysis was performed by using 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) connected to the Agilent HPLC-Chip system. Modification analysis of the active center cysteine was performed by means of Agilent MassHunter BioConfirm software. The SS- and SSS-binding levels of C61 and C64 within the LATDSFNEYIQSHDLVLAEFFAPWCGHCK peptide were detected by observations at m/z 835.6369 and 843.8814, respectively.

Nishimura A., Yoon S., Matsunaga T., Ida T., Jung M., Ogata S., Morita M., Yoshitake J., Unno Y., Barayeu U., Takata T., Takagi H., Motohashi H., van der Vliet A, & Akaike T. (2024). Longevity control by supersulfide-mediated mitochondrial respiration and regulation of protein quality. Redox Biology, 69, 103018.

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