Stanbio glucose liqui uv hexokinase kit

The Stanbio Glucose Liqui-UV (Hexokinase) Kit (Stanbio Laboratory, Boerne, TX) was used to determine plasma glucose concentrations following the manufacturer’s instructions. Plasma non-esterified fatty acid (NEFA) concentrations were also determined using a commercial kit (NEFA-HR (2) Kit, Fujifilm, Mountain View, CA) according to the manufacturer’s instructions. Plasma creatine kinase concentrations were determined using a Creatine Kinase Assay Kit (Abnova Corporation, Tapei, Taiwan). The kit is based on enzyme coupled reactions in which creatine phosphate and adenosine diphosphate (ADP) are converted to creatine and adenosine triphosphate (ATP) by the CK enzyme. The generated ATP is used to phosphorylate glucose by hexokinase to generate glucose-6-phosphate, which is then oxidized by NADP in the presence of glucose-6-phosphate dehydrogenase (G6P-DH). The NADH produced is proportional to the CK activity in the given plasma sample.

Plasma glucose concentrations were determined using the Stanbio Glucose Liqui-UV (Hexokinase) Kit (Stanbio Laboratory, Boerne, TX, USA). This analysis is based on two following reactions: (i) catalyzed by hexokinase, glucose and ATP form glucose-6-phosphate and ADP and (ii) in the presence of NAD, glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form 6-phosphogluconate and NADH. The increase in NADH concentration is directly proportionate to the glucose concentration and this can be determined spectrometrically at 340 nm. The assay was conducted following the procedure given by the manufacturer and the absorbances were determined using the Synergy HTX Microplate Reader (Bio-Tek, Winooski, VT, USA). The sensitivity of the Stanbio Glucose Liqui-UV Kit method is 2 mg/dL. Plasma non-esterified fatty acid (NEFA) concentrations were determined using the NEFA-HR (2) Kit (Fujifilm, Mountain View, CA, USA) according to the manufacturer’s instructions. The minimum detectable level of this method is 0.0014 mEq/L.