Caliper ivis lumina 2 imaging station

Arthritis was induced exactly as described above. Once paw swelling was evident (arthritis score = ~ 2), 50 mg/kg F3 antibody conjugated to AF647 was administered via IP injection. Whole animal fluorescence was measured at various times using a Caliper IVIS Lumina II Imaging station (PerkinElmer, Santa Clara, CA) coupled to an ISOON5160 Andor Nikon camera equipped with Living Image Software Version 4.0 (Oxford Instruments, Concord, MA).

Mice were injected via tail vein with 10 nmol of a fluorescent dye conjugate. Mice were sacrificed at 2, 4, and 6 h post-injection, and organs were removed (n = 1 per timepoint per conjugate for the initial UreterGlow-0, -3, -11, and -45 biodistribution studies and n = 3 per timepoint per conjugate for the UreterGlow-11, IR800CW, IR800BK, and ZW800-1 biodistribution studies). For urine analysis, mice were administered 10 nmol UreterGlow-11 via tail vein injection, sacrificed at various timepoints, and urine was removed from the bladder via syringe. The organs and urine were imaged using a Caliper IVIS Lumina II Imaging Station (PerkinElmer, Waltham, MA, USA) coupled with an ISOON5160 Andor Nikon camera equipped with Living Image Software Version 4.0 (PerkinElmer, Waltham, MA, USA). The settings were as follows: lamp level, high; excitation, 745 nm; emission, ICG; epi illumination; binning (M) 4; FOV, 12.5; f-stop, 4; acquisition time, 1 s.