Log In Sign up
The largest database of trusted experimental protocols

Lenalidomide

Manufactured by Santa Cruz Biotechnology
Visit Supplier
Sourced in United States

Lenalidomide is a small molecule that functions as an immunomodulatory agent. It is a synthetic derivative of thalidomide.

Automatically generated - may contain errors

Lab products found in correlation

Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Biacore 3000 system, by GE Healthcare (1 mentions) D4902, by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Melphalan, by Santa Cruz Biotechnology (1 mentions) Z vad fmk, by Medical & Biological Laboratories (1 mentions) Facsverse, by BD (1 mentions) Vorinostat, by Selleck Chemicals (1 mentions) Apicidin, by Merck Group (1 mentions) Bortezomib, by Cell Signaling Technology (1 mentions) Belinostat, by Merck Group (1 mentions) Trichostatin a, by Merck Group (1 mentions) Scriptaid, by Merck Group (1 mentions) Cytarabine, by Pfizer (1 mentions) Panobinostat, by Selleck Chemicals (1 mentions) Melphalan, by Merck Group (1 mentions) Thapsigargin, by Fujifilm (1 mentions) Stf 083010, by Merck Group (1 mentions) Carfilzomib, by Amgen (1 mentions) Perk inhibitor gsk2606414, by Selleck Chemicals (1 mentions)

6 protocols using lenalidomide

1

Kinetic Analysis of NPM1 Binding

Check if the same lab product or an alternative is used in the 5 most similar protocols
We determined the binding kinetics by a surface plasmon resonance (SPR) analysis with the Biacore 3000 system (GE Healthcare, Buckinghamshire, UK). All experiments were performed at 25°C using TBS buffer (25 mM Tris-HCl, pH 7.4, 137 mM NaCl, 3 mM KCl). Recombinant NPM1 was expressed in E. coli and purified. The NPM1 obtained (1.84 µM, 100 µL) was immobilized onto the sensor chip NTA (GE Healthcare). The measurements were performed under conditions of 662 resonance units of the ligand and at a flow rate of 30 µL/min. To determine the dissociation constants, we injected three different concentrations of TC11 and lenalidomide (Santa Cruz Biotechnology, Santa Cruz, CA). The injection periods for association and dissociation were 60 and 300 s, respectively. The binding data were analyzed with 1:1 binding with the mass transfer model in the BIA evaluation software ver. 4.1 (Biacore).
Matsushita M., Ozaki Y., Hasegawa Y., Terada F., Tabata N., Shiheido H., Yanagawa H., Oikawa T., Matsuo K., Du W., Yamada T., Hozumi M., Ichikawa D, & Hattori Y. (2015). A Novel Phthalimide Derivative, TC11, Has Preclinical Effects on High-Risk Myeloma Cells and Osteoclasts. PLoS ONE, 10(1), e0116135.
+ Open protocol
+ Expand
2

Thymic Cell Culture and Compound Screening

Check if the same lab product or an alternative is used in the 5 most similar protocols
Thymic cells were cultured as described (35 (link)). Briefly, enzymatically dispersed thymic cell suspensions were washed (and some aliquots irradiated with 1,250 rads from a 60Co source) and cryo-stored within a few hours of thymectomy. Subsequently, they were thawed carefully, and cultured at 6× 105 - 1× 106 cells per well in 96 well round-bottomed plates, without added stimulants, in 200 μL of RPMI medium containing 15% fetal bovine serum (Bodinco, the Netherlands), 50 U/mL penicillin, 50 U/mL streptomycin and 1 mM sodium pyruvate, at 37°C in humidified air with 5% CO2. Every 2 - 3 days, we removed (and stored) 90 μL of supernatant from each well, and replaced it with 100 μL of fresh medium ± any test drugs. Thymic cells were pre-cultured for 3 - 7 days, to allow recovery from the thawing procedure, adaptation to culture conditions and for measuring baseline Ab production before addition of test drugs.
We dissolved lyophilized bortezomib (Velcade, Janssen-Cilag B.V., Belgium) in sterile saline, dexamethasone (D4902; Sigma-Aldrich) in absolute ethanol, and lenalidomide (Santa Cruz Biotechnology; sc-218656) in dimethyl sulfoxide.
Gomez A.M., Willcox N., Vrolix K., Hummel J., Nogales-Gadea G., Saxena A., Duimel H., Verheyen F., Molenaar P.C., Buurman W.A., De Baets M.H., Martinez-Martinez P, & Losen M. (2014). Proteasome inhibition with bortezomib depletes plasma cells and specific autoantibody production in primary thymic cell cultures from early-onset myasthenia gravis patients. Journal of immunology (Baltimore, Md. : 1950), 193(3), 1055-1063.
+ Open protocol
+ Expand
3

Cytotoxicity and Apoptosis Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cytotoxicity of the compounds was analyzed by Cell Counting Kit-8 (Dohjin Chemicals, Kumamoto, Japan) or trypan blue dye exclusion assay according to the manufacturer's protocol. The MEBCYTO Apoptosis kit was used to detect apoptosis by flow cytometry using FACSVerse (BD Biosciences, Franklin Lakes, NJ, USA) according to the manufacturer's protocol. Melphalan and lenalidomide were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA) and Sigma-Aldrich; Merck KGaA, (Darmstadt, Germany), respectively. A pan-caspase inhibitor, ZVAD-fmk (Medical and Biological Laboratories, Nagoya, Japan), was utilized in some experiment at a concentration of 32 nM.
Fujii E., Inada Y., Kakoki M., Nishimura N., Endo S., Fujiwara S., Wada N., Kawano Y., Okuno Y., Sugimoto T, & Hata H. (2018). Bufalin induces DNA damage response under hypoxic condition in myeloma cells. Oncology Letters, 15(5), 6443-6449.
+ Open protocol
+ Expand
4

Cytarabine storage and epigenetic compound libraries

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cytarabine (147–94-4, Pfizer, New York, NY, USA) was aliquoted and stored at 4 °C with bulk concentrations of 411 mM. SCREEN-WELL® Epigenetics library (BML-2836, Enzo LifeSciences Inc., Farmingdale, NY, USA) and Chemotherapeutic Agent Library (L1500, Selleck, Munich, Germany) were stored at − 80 °C until use. Substances used for proliferation studies including bortezomib (2204S, Cell Signaling Technologies), lenalidomide (PCID-216326 Santa Cruz Biotechnology, USA), apicidin (A8851, Sigma Aldrich), belinostat (PXD101), M-344 (M5820, Sigma Aldrich), oxamflatin (O3139, Sigma Aldrich, St. Louis, MO, USA), scriptaid (S7817, Sigma Aldrich), trichostatin A (T1952, Sigma Aldrich) and vorinostat (SAHA MK0683, Selleck) were dissolved in DMSO (Sigma Aldrich), aliquoted and stored at − 80 °C until use.
Freiburghaus C., Emruli V.K., Johansson A., Eskelund C.W., Grønbæk K., Olsson R., Ek F., Jerkeman M, & Ek S. (2018). Bortezomib prevents cytarabine resistance in MCL, which is characterized by down-regulation of dCK and up-regulation of SPIB resulting in high NF-κB activity. BMC Cancer, 18, 466.
+ Open protocol
+ Expand
5

Solubilization of Anticancer Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
Melphalan (Sigma-Aldrich), lenalidomide (Santa Cruz Biotechnology, Dallas, TX, USA), panobinostat (Selleck Chemicals, Houston, TX, USA), bortezomib (Selleck Chemicals), and carfilzomib (Amgen, Thousand Oaks, CA, USA) were dissolved in dimethyl sulfoxide (DMSO) (Sigma-Aldrich) and kept frozen at −20 °C. Dexamethasone (MSD, Tokyo, Japan) was stored at 4 °C until use. Thapsigargin (Wako, Osaka, Japan), PERK inhibitor (GSK2606414; Selleck Chemicals) and IRE1α inhibitor (STF083010; Sigma-Aldrich) were also dissolved with DMSO and stored at −20 °C.
Matsushita M., Kashiwazaki S., Kamiko S., Kobori M., Osada M., Kunieda H., Hirao M., Ichikawa D, & Hattori Y. (2023). Immunomodulatory Effect of Proteasome Inhibitors via the Induction of Immunogenic Cell Death in Myeloma Cells. Pharmaceuticals, 16(10), 1367.
+ Open protocol
+ Expand
6

Combination Immunomodulatory Drug Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following reagents were purchased from the indicated manufacturers: panobinostat (LBH589) and ACY-1215 from Cayman Chemical Company (MN, USA); entinostat (MS-275) from Selleck (Houston, USA); lenalidomide from Santa Cruz Biotechnology (CA, USA); pomalidomide from Toronto Research Chemicals (Toronto, Canada); recombinant human (rh) IFN-γ from R&D systems (MN, USA); rh IL-6 from Pepro Tech Inc. (London, UK) and MG132 from Selleck (Houston, USA); phycoerythrin (PE)-conjugated monoclonal antibodies (mAbs) against human PD-L1 (#329706), human MICA/MICB (#320906), human CD119 (IFN-γR1) (#308606) and mouse IgG1-κ isotype control (#400114) from BioLegend (CA, USA); human ULBP-2/5/6 (FAB1298P) from R&D systems (MN, USA); fluorescein isothiocyanate (FITC)-conjugated mAbs against human CD38 (#555459) from BD Biosciences (CA, USA); anti-phosho-STAT1 (Tyr701) antibody (#7649), anti-STAT1 antibody (#14995), anti-PD-L1 antibody (#13684) and anti-GAPDH antibody (#5174) from Cell Signaling; anti-IRF1 antibody (sc-497) from Santa Cruz (CA, USA); and anti-β-actin antibody (A5316) from Sigma-Aldrich (St. Louis, MO, USA); 17-AAG from Calbiochem EMD Biosciences, Inc. (La Jolla, CA, USA).
Iwasa M., Harada T., Oda A., Bat-Erdene A., Teramachi J., Tenshin H., Ashtar M., Oura M., Sogabe K., Udaka K., Fujii S., Nakamura S., Miki H., Kagawa K., Ozaki S, & Abe M. (2019). PD-L1 upregulation in myeloma cells by panobinostat in combination with interferon-γ. Oncotarget, 10(20), 1903-1917.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!