Fetal bovine serum (FBS), Cell Dissociation Reagent, medium LHC-9 and Dulbecco’s modified Eagle medium (DMEM) were provided by Invitrogen (Carlsbad, CA). The antibody against VECTOR ImmPRESS Anti-Goat Ig was provided by Santa Cruz Biotechnology (Santa Cruz, CA). Protein Block solution and DAB substrate were provided by Abcam (Cambridge, MA). Antibodies against vimentin, E-cadherin, N-cadherin and TCTP were purchased from Cell Signaling (Boston, MA). ECL reagent kit was purchased from GE Healthcare Life Sciences (Piscataway, NJ). NNK was purchased from Chemsyn Science Laboratories (Lenexa, KS). 10 µM NNK was used in the experiments [20 (link)]. PM2.5 was collected at Kowloon Tong of Hong Kong and 5 µg/ml PM2.5 was used in the experiments as details described in our previous study [21 (link)].
Antibodies against vimentin
Manufactured by Cell Signaling Technology
Sourced in United States
Antibodies against vimentin are laboratory reagents used to detect and quantify the presence of the vimentin protein. Vimentin is a type III intermediate filament protein that is commonly used as a marker for mesenchymal cells. These antibodies can be used in various techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to study the expression and localization of vimentin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
E cadherin, by Cell Signaling Technology (2 mentions)
Protein block solution, by Abcam (1 mentions)
Dab substrate, by Abcam (1 mentions)
Cell dissociation reagent, by Thermo Fisher Scientific (1 mentions)
Ecl reagent kit, by GE Healthcare (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
N cadherin, by Cell Signaling Technology (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Af 101 na, by R&D Systems (1 mentions)
Af 291 na, by R&D Systems (1 mentions)
Mab275r, by R&D Systems (1 mentions)
Mab294, by R&D Systems (1 mentions)
Gw3965, by Merck Group (1 mentions)
Cck 8 cell viability kit, by Dojindo Laboratories (1 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Gefitinib, by AstraZeneca (1 mentions)
Phenylmethylsulfonyl fluoride, by Roche (1 mentions)
Nitrocellulose membrane, by Merck Group (1 mentions)
Ripa protein extraction reagent, by Beyotime (1 mentions)
4 protocols using antibodies against vimentin
1
Epithelial-Mesenchymal Transition Induction
2
Neutralizing Antibodies for Cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Unless otherwise stated, all chemicals and plastics were obtained from Sigma-Aldrich (St. Louis, MO, USA). Neutralizing antibodies against EGF (# AF236), GRO-1 (# MAB275R), HGF (# MAB294), IGF-1 (# AF-291-NA), and TGFβ1 (# AF-101-NA), as well as the antibody detecting ICAM-1 (# BBA3), were obtained from R&D Systems (Abingdon, UK). Anti-integrin α5β1 antibody (# AB1999) and isotype control antibody (# CBL600) were purchased from Merck (Dorset, UK). Antibodies against vimentin (# 5741) were obtained from Cell Signaling Technology (Beverly, MA, USA). Integrin-linked kinase inhibitor (Cpd22) was purchased from Sigma-Aldrich. Exogenous, recombinant human TGF-β1, HGF, GRO-1, and IGF-1 were obtained from R&D Systems.
3
Evaluating Gefitinib's Impact on Lung Cancer Cell Viability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Reagents and suppliers were as follows: gefitinib (AstraZeneca UK Limited, Macclesfield, Cheshire, UK); CCK-8 cell viability kit (Dojindo, Kumamoto, Japan); GW3965 (Sigma-Aldrich Co., St Louis, MO, USA); penicillin–streptomycin solution (Thermo Fisher Scientific, Waltham, MA, USA); antibodies against vimentin, E-cadherin, β-actin, and EGFR (Cell Signaling Technology, Danvers, MA, USA); cell lines, HCC827, H1299, H1975, H1650, and H358 (Shanghai Institutes for Biological Sciences, China Academy of Cell Resource Center).
4
Western Blot Analysis of EMT Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were lysed using RIPA protein extraction reagent (Beyotime, Beijing, China) supplemented with a protease inhibitor cocktail (Roche, Pleasanton, CA, USA) and phenylmethylsulfonyl fluoride (Roche). The concentration of proteins was determined using the Bio-Rad (Hercules, CA, USA) protein assay kit. Protein extracts (50 μg) were separated by 10% SDS-polyacrylamide gel electrophoresis, then transferred to nitrocellulose membranes (Sigma-Aldrich) and incubated with antibodies. Autoradiograms were quantified by densitometry using Quantity One software (Bio-Rad) using GAPDH as a control. Antibodies (1 : 1000 dilution) against E-cadherin and N-cadherin were purchased from BD Biosciences. Antibodies against vimentin was purchased from Cell Signaling Technology (Danvers, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!