The human RPE cell line ARPE-19 was obtained from the American Type Culture Collection (Manassas, VA, USA). TRIzol reagent and Dulbecco's modified Eagle's medium (DMEM)/F12 culture medium were purchased from Life Technologies (Grand Island, NY, USA); iScript cDNA synthesis kit from Bio-Rad Laboratories (Hercules, CA, USA); DreamTaq Green PCR Master Mix from Thermo Scientific (Pittsburgh, PA, USA); fetal bovine serum, penicillin, and streptomycin from Life Technologies (Grand Island, NY, USA); rabbit polyclonal anti-GPR91 antibody from Alpha Diagnostic International (San Antonio, TX, USA); antibodies against Smad1/5 and phospho-Smad1/5/8 from Cell Signaling Technology, Inc., (Danvers, MA, USA); antibody against phospho-Smad4 from Abgent, Inc., (San Diego, CA, USA); and secondary antibodies (goat anti-rabbit IgG conjugated with Alexa Fluor 568 and goat anti-rabbit IgG coupled to Alexa Fluor 488) from Molecular Probes (Carlsbad, CA, USA).
Dulbecco s modified eagle s medium dmem f12 culture medium
Manufactured by Thermo Fisher Scientific
Sourced in United States
Dulbecco's modified Eagle's medium (DMEM)/F12 culture medium is a cell culture medium widely used for the in vitro cultivation of various cell types. It provides a balanced salt solution and a combination of nutrients required for the growth and maintenance of cells.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Dreamtaq green pcr master mix, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Iscript cdna synthesis kit, by Bio-Rad (1 mentions)
Arpe 19, by American Type Culture Collection (1 mentions)
B27 supplement, by Thermo Fisher Scientific (1 mentions)
Cy3 conjugated goat anti rabbit antibody, by Merck Group (1 mentions)
Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (1 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Anti β actin antibody, by Abcam (1 mentions)
Anti caspase 3 antibody, by Abcam (1 mentions)
2 protocols using dulbecco s modified eagle s medium dmem f12 culture medium
1
ARPE-19 Cell Line Characterization
2
Isolation and Culture of Neural Stem Cells
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Dulbecco’s modified Eagle’s medium (DMEM)/F12 culture medium was purchased from Life Technologies (Gaithersburg, MD, USA). Fetal bovine serum (FBS) and B27 supplement were purchased from Gibco-BRL (Grand Island, NY, USA). Basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) were obtained from PeproTech (Rocky Hill, NJ, USA). Normal goat serum was provided by Wuhan Boshide Biotechnology Co., Ltd. (Wuhan, China). Poly-L-lysine, 4′,6-diamidino-2-phenylindole (DAPI), fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit antibody and Cy3-conjugated goat anti-rabbit antibody were provided by Sigma (St. Louis, MO, USA). Rabbit anti-mouse CD133 monoclonal antibody and glial fibrillary acidic protein (GFAP) polyclonal antibody were purchased from Abcam (Cambridge, MA, USA) and Zhongshan Jinqiao Biotechnology, Ltd., (Beijing, China), respectively. Anti-caspase-3 antibody (rabbit polyclonal against mouse, rat or human) and anti-β-actin antibody were obtained from Abcam Inc. (Cambridge, MA, USA). Cell Counting kit-8 (CCK-8) was purchased from Dojindo Laboratories (Tokyo, Japan).
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