Camera control unit ds l2

Immunohistochemical staining was performed for Ki-67 and cleaved caspase-3. Slides were stained as described previously (Matsuu-Matsuyama et al., 2010) . Anti-rat Ki-67 monoclonal antibody (MIB-5) (Agilent Dako, CA, USA) and cleaved caspase-3 antibody (Cell Signaling Technology Inc., MA, USA) were used. The secondary antibody, LSAB-2 system-HRP for use on rat specimens (Agilent Dako, CA, USA), was used according to the manufacturer's instructions.
Cells showing immunoreactivity for Ki-67 and cleaved caspase-3 were counted in more than 30 crypts and 50 villi of the jejunum per rat at 400× magnification using light microscopy. The cleaved caspase-3 positive bone marrow cells were counted in five or six areas from four rats in every group at 400× magnification captured with light microscopy and a digital camera system (Nikon Camera Control Unit DS-L2).

Apoptosis was confirmed using a TUNEL technique (Apoptag Peroxidase In Situ Apoptosis Detection Kit, EMD Millipore Co., CA, USA) that detects apoptosis-associated DNA fragmentation by labeling the 3'-OH termini with digoxigenin nucleotides using terminal deoxynucleotidyl transferase. Slides were stained as described previously (Matsuu-Matsuyama et al., 2010) . TUNEL positive cells were counted in more than 30 crypts and 50 villi of the jejunum per rat at 400× magnification using light microscopy. TUNEL positive bone marrow cells were counted in five or six areas per rat at 400× magnification captured with light microscopy and a digital camera system (Nikon Camera Control Unit DS-L2).