Cd14 allophycocyanin apc clone m5e2

Manufactured by BD

Sourced in Germany

CD14-allophycocyanin (APC; clone M5E2) is a fluorophore-conjugated antibody that binds to the CD14 cell surface receptor. CD14 is a glycosylphosphatidylinositol-anchored protein that serves as a co-receptor for the detection of bacterial lipopolysaccharide. The APC fluorophore conjugated to the CD14 antibody allows for the detection and analysis of CD14-expressing cells using flow cytometry techniques.

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Lab products found in correlation

Cd105 fluorescein isothiocyanate fitc clone mem 226, by Immunotools (2 mentions) Facscanto 2, by BD (2 mentions) Cd73 pe clone ad2, by BD (1 mentions) Cd34 apc clone 8g12, by BD (1 mentions) Cd31 pe clone wm59, by BD (1 mentions) Cd45 apc clone hi30, by BD (1 mentions) Cd90 apc clone 5e10, by BD (1 mentions)

Spelling variants of this product

CD14-APC (49 citations) CD14 (clone M5E2, (21 citations) CD14 (M5E2, (13 citations) CD14-APC (clone M5E2, (5 citations) CD14 allophycocyanin (4 citations) APC clone M5E2 (3 citations)

2 protocols using cd14 allophycocyanin apc clone m5e2

Immunophenotypic Characterization of Cells

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Surface marker expression was analysed with a FACS Canto II (BD Biosciences, NJ, USA). The following antibodies were used for immunophenotypic analysis: CD14-allophycocyanin (APC; clone M5E2), CD29-phycoerythrin (PE; clone MAR4), CD31-PE (clone WM59), CD34-APC (clone 581), CD45-APC (clone HI30), CD73-PE (clone AD2), CD90-APC (clone 5E10; all from BD Biosciences) and CD105-fluorescein isothiocyanate (FITC; clone MEM-226; ImmunoTools, Friesoythe, Germany).

Göbel C., Goetzke R., Eggermann T, & Wagner W. (2018). Interrupted reprogramming into induced pluripotent stem cells does not rejuvenate human mesenchymal stromal cells. Scientific Reports, 8, 11676.

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Immunophenotypic Surface Marker Analysis

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Immunophenotypic surface marker analysis was performed on a FACS Canto II (BD, Heidelberg, Germany) upon staining with CD14 allophycocyanin (APC, clone M5E2; BD), CD29 phycoerythrin (PE, clone MAR4; BD), CD31 PE (clone WM59; BD), CD34 APC (clone 8G12; BD), CD45 APC (clone HI30; BD), CD73 PE (clone AD2; BD), CD90 APC (clone 5E10; BD), CD105 fluorescein isothiocyanate (FITC, clone MEM-226; ImmunoTools, Friesoythe, Germany).

Fernandez-Rebollo E., Mentrup B., Ebert R., Franzen J., Abagnale G., Sieben T., Ostrowska A., Hoffmann P., Roux P.F., Rath B., Goodhardt M., Lemaitre J.M., Bischof O., Jakob F, & Wagner W. (2017). Human Platelet Lysate versus Fetal Calf Serum: These Supplements Do Not Select for Different Mesenchymal Stromal Cells. Scientific Reports, 7, 5132.

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