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Mouse cxcl10 elisa kit

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The Mouse CXCL10 ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse CXCL10 levels in cell culture supernatants, serum, and plasma.

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Lab products found in correlation

Recombinant murine ifn γ, by Thermo Fisher Scientific (1 mentions) Ifn γ, by R&D Systems (1 mentions)

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ELISAs (144 citations) Mouse ELISA kits (102 citations) CXCL10 (92 citations) CXCL10 ELISA kits (5 citations) Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA kit (3 citations) Mouse CXCL10 (2 citations) Mouse CXCL10 Quantikine ELISA kit (2 citations)

3 protocols using mouse cxcl10 elisa kit

1

Investigating CXCL10 Secretion in WT and KO Cells

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WT (MB49, Ctrl # 116 and 376) and KO (KO # 175, 345 and 377) cells were cultured in normal media supplemented or not with recombinant murine IFN-γ (#315–05, PeproTech): 0, 2, 5, 10 and 25 ng/mL. After 72 h of IFN-γ treatment, supernatants were collected and secreted CXCL10 was assayed with mouse CXCL10 ELISA kit (#DY466-05, #DY008, R&D Systems) according to the manufacturer’s instructions.
Baloche V., Rivière J., Tran T.B., Gelin A., Bawa O., Signolle N., Diop M.K., Dessen P., Beq S., David M, & Busson P. (2021). Serial transplantation unmasks galectin-9 contribution to tumor immune escape in the MB49 murine model. Scientific Reports, 11, 5227.
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2

Quantifying CXCL10 in Melanoma Xenografts

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To assess the concentration of CXCL10 in NSG mice, human melanoma cell lines were xenotransplanted in NSG mice. When the tumors reached 10 mm in diameter, the mice were sacrificed and tumor tissue and spleens were harvested from individual mice. Tissue was then pressed through a 70 μm filter and lysed in TPER buffer. The concentration of mouse CXCL10 in these tumor and spleen suspensions was then evaluated by a mouse CXCL10 ELISA kit (R&D Systems, Minneapolis, MN), per manufacturer’s instructions.
Jenkins M.H., Brinckerhoff C.E, & Mullins D.W. (2015). CXCR3 Signaling in BRAFWT Melanoma Increases IL-8 Expression and Tumorigenicity. PLoS ONE, 10(3), e0121140.
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3

CXCL10 Secretion Quantification

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The level of CXCL10 secreted into the supernatant was measured using the Mouse CXCL10 ELISA Kit (DY466-05, R&D Systems) and Human CXCL10 ELISA Kit (JL28903, Jianglai Biological) according to the manufacturer’s instructions.
Jie X., Chen Y., Zhao Y., Yang X., Xu Y., Wang J., Meng R., Zhang S., Dong X., Zhang T., Yang K., Xu S, & Wu G. (2022). Targeting KDM4C enhances CD8+ T cell mediated antitumor immunity by activating chemokine CXCL10 transcription in lung cancer. Journal for Immunotherapy of Cancer, 10(2), e003716.
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