Penicillin streptomycin

Normal colon cells (FHC) and CRC cells (HCT116, HT29, SW480, and SW620; Shanghai Cell Bank, Shanghai, China) were cultured in Dulbecco’s Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and penicillin/streptomycin (100 U/mL) in an incubator with 5% CO₂ at 37 °C. In addition, 293 T cells obtained from the American Type Culture Collection were cultured in Roswell Park Memorial Institute 1640 Medium with 10% FBS and penicillin/streptomycin (100 U/mL; Gibco, USA). METTL3 short hairpin RNA plasmid, CRB3 short hairpin RNA plasmid, or negative control (Genomeditech, China) were transfected using FuGene HD transfection reagent (E2311, Promega, USA) per the protocol used in a previous study [27 (link)].

The cell lines A549 and NCI‐H1975 were obtained from American Type Culture Collection (Rockville, MD, USA) and grown in RPMI‐1640 medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Gibco, Carlsbad, CA, USA). HEK‐293T was a generous gift from Prof. Chung Hang LEUNG (University of Macau). HEK‐293T cells were grown in Dulbecco's Modified Eagle Medium containing 10% fetal bovine serum. The lung fibroblast cell line MRC‐5, grown in Minimum Essential Medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin, was obtained from Shanghai Cell Bank (Shanghai, China). The cells were cultured in a humidified incubator containing 5% CO₂ at 37°C. The short tandem repeat (STR) DNA profiling analysis was used for human lung cancer cell line authentication (Shanghai Genesky Biotechnologies Inc.) on July 19, 2019.