Multicolor flow cytometric analysis was performed on isolated cells according to standard procedures using human mAbs that cross-react with PTM and RM. Cells were stained with Aqua Live/Dead amine dye (Invitrogen) to identify dead cells. Predetermined optimal concentrations were then used of the following antibodies: anti-CD3-Alexa700 (clone SP34-2, BDPharmigen), anti-CD8 PacBlue (clone RPA-T8, BDPharmigen), anti-CD4 PE-Cy5.5 (clone OKT4, eBioscience), anti-CD4 PE-Cy5.5 (clone L200, BDPharmigen), anti-Ki67 FITC (clone B26, BDPharmigen), anti-CCR5 PE (clone 3A9, BDPharmigen), anti-CD28 ECD (clone 28.2, Beckman Coulter), anti-CD95 PE-Cy5 (clone DX2, BDPharmigen), anti-CD14 APC (clone M5E2, BDPharmigen), All samples were permeabilized and fixed using CytoFix/Perm Kit (BDPharmigen) and intracellularly stained to detect Ki67. The acquired data were anlalyzed using FlowJo software (Treestar).
Anti cd4 pe cy5.5 clone okt4
Manufactured by Thermo Fisher Scientific
The Anti-CD4 PE-Cy5.5 (clone OKT4) is a fluorescently-labeled monoclonal antibody that binds to the CD4 receptor on T cells. It is designed for use in flow cytometry applications to identify and quantify CD4-positive cells within a sample.
Automatically generated - may contain errors
Lab products found in correlation
Live dead aqua amine dye, by Thermo Fisher Scientific (2 mentions)
Cytofix perm kit, by BD (2 mentions)
Anti cd95 pe cy5, by BD (2 mentions)
Anti cd3 alexa700 clone sp34 2, by BD (2 mentions)
Anti cd8 pacblue clone rpa t8, by BD (2 mentions)
Anti cd4 pe cy5.5 clone l200, by BD (2 mentions)
Anti ki67 fitc clone b26, by BD (2 mentions)
Anti ccr5 pe clone 3a9, by BD (2 mentions)
Anti cd28 ecd clone 28, by Beckman Coulter (2 mentions)
Anti cd14 apc clone m5e2, by BD (2 mentions)
2 protocols using anti cd4 pe cy5.5 clone okt4
1
Multicolor Flow Cytometry for T-Cell Phenotyping
2
Multicolor Flow Cytometry for T-Cell Phenotyping
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Multicolor flow cytometric analysis was performed on isolated cells according to standard procedures using human mAbs that cross-react with PTM and RM. Cells were stained with Aqua Live/Dead amine dye (Invitrogen) to identify dead cells. Predetermined optimal concentrations were then used of the following antibodies: anti-CD3-Alexa700 (clone SP34-2, BDPharmigen), anti-CD8 PacBlue (clone RPA-T8, BDPharmigen), anti-CD4 PE-Cy5.5 (clone OKT4, eBioscience), anti-CD4 PE-Cy5.5 (clone L200, BDPharmigen), anti-Ki67 FITC (clone B26, BDPharmigen), anti-CCR5 PE (clone 3A9, BDPharmigen), anti-CD28 ECD (clone 28.2, Beckman Coulter), anti-CD95 PE-Cy5 (clone DX2, BDPharmigen), anti-CD14 APC (clone M5E2, BDPharmigen), All samples were permeabilized and fixed using CytoFix/Perm Kit (BDPharmigen) and intracellularly stained to detect Ki67. The acquired data were anlalyzed using FlowJo software (Treestar).
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