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Vitek 2 gn ast n222

Manufactured by bioMérieux
Sourced in France

The VITEK 2 GN AST-N222 is a laboratory instrument used for the rapid identification and antimicrobial susceptibility testing of Gram-negative bacteria. It is designed to provide automated and standardized testing for clinical microbiology laboratories.

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2 protocols using vitek 2 gn ast n222

1

Bacterial Identification and Antimicrobial Susceptibility Testing

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Cultures were performed according to the working protocol of the Bacteriology Laboratory of SCJUPBT. All isolates were first identified using the VITEK® 2 GN, VITEK® 2 GP ID cards (BioMérieux, Marcy l’Etoile, France). Antimicrobial susceptibility tests (AST) were performed using the VITEK 2 GN AST-N222 and VITEK 2 AST GP 67 cards (BioMérieux, Marcy l’Etoile, France) by determination of the minimum inhibitory concentration (MIC) and classification into resistance phenotypes, according to the Clinical Laboratory and Standards Institute (CLSI) 2020/2021 criteria. The reference strains used were: Escherichia coli ATCC 25922 and Klebsiella pneumoniae ATCC 1705, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923. Clostridioides difficile enterocolitis was diagnosed by determining the A and/or B toxin from the spontaneously emitted feces by using the LIAISON® Analyzer through an automatic immunoassay testing.
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2

Sputum-Based Microbiological Analysis

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Expectorate sputum was selected as the sampling technique and routine microbiological investigations were conducted at the medical microbiology laboratory using standard bacteriology. All isolates were first identified using the VITEK® 2 GN and VITEK® 2 GP ID cards (BioMérieux, Marcy, l’Etoile, France). Antimicrobial susceptibility tests were performed using the VITEK 2 GN AST-N222 and VITEK 2 AST GP 67 cards (BioMérieux, Marcy, l’Etoile, France).
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