Mcp 500 polarimeter

Manufactured by Anton Paar

Sourced in Austria

The MCP 500 polarimeter from Anton Paar is a laboratory instrument designed to measure the optical rotation of samples, which is a fundamental physical property of many organic and inorganic compounds. It is capable of determining the specific rotation of a substance with high precision and accuracy.

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Lab products found in correlation

Maxis q tof mass spectrometer, by Bruker (3 mentions) Chirascan circular dichroism spectrometer, by Applied Photophysics (2 mentions) Drx 500 spectrometer, by Bruker (2 mentions) Silica gel, by Qingdao Marine Chemical (2 mentions) Uv 2600 pc spectrometer, by Shimadzu (2 mentions) Iraffinity 1 spectrophotometer, by Shimadzu (2 mentions) Ir affinity 1 spectrometer, by Shimadzu (1 mentions) Biotek synergy h1, by Agilent Technologies (1 mentions) Av 400 spectrometer, by Bruker (1 mentions) 1525 pump, by Waters Corporation (1 mentions) Orbitrap fusion high resolution mass spectrometer, by Thermo Fisher Scientific (1 mentions) Thermo hypersil c18 column, by Waters Corporation (1 mentions) Chirascan, by Applied Photophysics (1 mentions) 2487 dual wavelength detector, by Waters Corporation (1 mentions) Toyopearl hw 40f, by Tosoh (1 mentions) Avance 3, by Bruker (1 mentions) Merck tlc silica gel 60 f254, by Merck Group (1 mentions) Lh 20, by GE Healthcare (1 mentions) Uv 2600 spectrophotometer, by Shimadzu (1 mentions) 1260 infinity system, by Agilent Technologies (1 mentions)

Spelling variants of this product

MCP 500 (7 citations) MCP 500 Modular Circular Polarimeter (2 citations) MCP 500 automatic polarimeter (2 citations)

7 protocols using mcp 500 polarimeter

Quantitative Phase Diagram Analysis

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After centrifugation,
part of each
phase was isolated. The composition of each phase was measured using
polarimetry^{30 (link)} at four wavelengths (589,
546, 436, and 365 nm) on an Anton Paar MCP 500 polarimeter, allowing
simultaneous determination of the concentration of dextran and gelatin
of each isolated phase. For an elaborate description of the procedure,
see ref (31 (link)). We verified
that the specific rotation of dextran is independent of molar mass;
thereby, our measurements on systems containing both 20 and 70 kDa
dextran yield their combined mass fraction in each phase. These measurements
allow construction of phase diagrams and computation of the tie-line
length defined in terms of the difference in polymer mass fraction
between the two phases where w_i^j indicates the (total)
mass fraction of dextran (A) or gelatin (B)
in each phase (denoted α and β).

Vis M., Blokhuis E.M., Erné B.H., Tromp R.H, & Lekkerkerker H.N. (2017). Interfacial Tension of Phase-Separated Polydisperse Mixed Polymer Solutions. The Journal of Physical Chemistry. B, 122(13), 3354-3362.

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Comprehensive Analytical Characterization Protocol

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Optical rotations were determined using an AntonPaar MCP500 polarimeter. UV spectra were measured with a Shimadzu UV-2600 PC spectrometer. IR spectra were recorded on a Shimadzu IR Affinity-1 spectrometer with KBr pellets. 1D and 2D NMR spectra were collected on a Bruker DRX-500 spectrometer, δ in ppm rel. to TMS, J in Hz. HRESIMS were performed using a Bruker maXis TOF-Q mass spectrometer (Bruker, Daltonics, Billerica, MA, USA). Silica gel (100–200 mesh, 200–300 mesh, Qingdao Marine Chemical Ltd., Qingdao, China), Sephadex LH-20 (GE Healthcare Bio-sciences AB, Uppsala, Sweden), YMC*GEL ODS-A (S-50 μm, 12 nm) (YMC Co., Ltd., Kyoto, Japan) were used for column chromatography. Semipreparative HPLC was performed using an ODS column (YMC-ODS-A, 250 × 10 mm, 5 μm). CD spectra were measured on a Biologic MOS-450 spectra polarimeter (Biologic Science, Claix, France). ECD spectra were measured with a Chirascan circular dichroism spectrometer (Applied Photophysics). MTT and antimicrobial assays were analyzed using a microplate reader (BioTek Synergy H1, BioTek Instruments, Inc., Winooski, VT, USA).

Lei H., Bi X., Lin X., She J., Luo X., Niu H., Zhang D, & Yang B. (2021). Heterocornols from the Sponge-Derived Fungus Pestalotiopsis heterocornis with Anti-Inflammatory Activity. Marine Drugs, 19(11), 585.

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Comprehensive Analytical Techniques for Compound Characterization

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Optical rotation was measured with an MCP-500 polarimeter (Anton Paar). The UV spectrum was recorded with a TU-1810DSPC UV-Vis spectrometer (Puxi Tongyong). IR spectrum was measured on a IR Affinity-1 spectrophotometer (Shimadzu); CD was measuredon a Chirascan^TM (Applied Photophysics Ltd., Leatherhead, UK). NMR was acquired on an AV-400 spectrometer (Bruker) with TMS as internal standard, J in Hz; HRESIMS spectra were measured on the Orbitrap Fusion high resolution mass spectrometer (Thermo) equipped with ESI source. Preparative or semi-preparative HPLC were performed on a HPLC system equipped with a Waters 1525 pump and a Waters 2487 Dual Wavelength Detector using a Thermo hypersil C₁₈ column (5 μm, 150 × 25.0 mm, i.d.) or Thermo hypersil C₁₈ column (5 μm, 250 × 10 mm, i.d.). Open column chromatography was performed using silica gel (200–300 mesh, Qingdao Marine Chemical, Qingdao, China), Sephadex LH-20 (25–100 μm, Fiji), MCI gel CHP 20P (75–150 μm, Fiji), Toyopearl HW 40F (TOSOH, Tokyo, Japan) or ODS (40–63 μm, Merck, Kenilworth, NJ, USA).

Zhong C., Hu D., Hou L.B., Song L.Y., Zhang Y.J., Xie Y, & Tian L.W. (2017). Phenolic Compounds from the Rhizomes of Smilax china L. and Their Anti-Inflammatory Activity. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(4), 515.

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Synthesis and Characterization of Novel Compounds

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Commercially available chemicals used in this work were purchased from Sigma-Aldrich (Darmstadt, Germany) and were used as supplied, without additional purification. NMR spectra were recorded in CDCl₃ on a Bruker Avance III (600 MHz for 1H NMR, 150 MHz for 13C NMR) (Billerica, MA, USA); coupling constants are reported in hertz (Hz). The rotations were measured using an Anton Paar MCP 500 polarimeter (Anton Paar GmbH, Graz, Austria). Melting points are uncorrected. Chromatographic purification of compounds was achieved with 230−400 mesh size silica gel. The progress of reactions was monitored by silica gel thin-layer chromatography plates (Merck TLC Silicagel 60 F254) (Merck Millipore, Darmstadt, Germany).
Copies of 1H and 13C NMR spectra of compounds 9–20 are included in the Supplementary Material.

Tracz A., Malinowska M., Leśniak S, & Zawisza A. (2022). Aziridine Ring Opening as Regio- and Stereoselective Access to C-Glycosyl-Aminoethyl Sulfide Derivatives. Molecules, 27(6), 1764.

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Instrumental Analysis of Molecular Compounds

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Optical rotations were measured on an AntonPaar MCP500 polarimeter. IR spectra were performed on a Shimadzu IR spectrometer with KBr disk. HRESIMS were used for a Bruker maXis TOF-Q mass spectrometer. Bruker DRX-500 spectrometer was used to measure the NMR spectra. The UV spectra were recorded on a Shimadzu UV-2600 PC spectrometer. Silica gel (100–200 mesh, 200–300 mesh, Qingdao, China), YMC*GEL ODS-A (S-50 μm, 12 nm) (YMC Co., Ltd., Kyoto, Japan), Sephadex LH-20 (GE, Sweden) were used for column chromatography. Semipreparative HPLC was used for ODS column (YMC-ODS-A). CD spectra were performed on a Biologic MOS-450 spectra polarimeter.

Lei H., Zou S., Lin J., Zhai L., Zhang Y., Fu X., Chen S., Niu H., Liu F., Wu C, & Zhang D. (2022). Antioxidant and anti-inflammatory activity of constituents isolated from Dendrobium nobile (Lindl.). Frontiers in Chemistry, 10, 988459.

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Spectroscopic Analysis of Compounds

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Optical rotations were measured with a MCP 500 polarimeter (Anton Paar, Graz, Austria). UV spectra were recorded using a UV-2600 spectrophotometer (Shimadzu, Kyoto, Japan). ECD spectra were measured with a Chirascan circular dichroism spectrometer (Applied Photophysics, Ltd, London, UK). IR spectra were determined with a FT-IR-NICOLET spectrophotometer. 1 H, 13 C NMR and 2D NMR spectra were acquired with a Bruker AV-500/700 MHz

Nong X.H., Wei X.Y, & Qi S.H. (2017). Pteridic acids C-G spirocyclic polyketides from the marine-derived Streptomyces sp. SCSGAA 0027. The Journal of antibiotics, 70(11).

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Analytical Techniques for Natural Product Isolation

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Column chromatography (CC) was performed by using silica gel (100-200 mesh; Jiangpeng Silica Gel Development, Inc., Yantai, China) or Sephadex LH-20 (40-70 μm; GE Healthcare, Uppsala, Sweden). TLC was conducted with precoated glass plates (0.1-0.2 mm; silica gel GF 254 , 10-40 nm, Jiangpeng). HPLC analyses were performed with a 1260 infinity system (Agilent, Santa Clara, CA, USA) using a Phenomenex Prodigy ODS (2) column (150 × 4.6 mm, 5 μm; Torrance, CA, USA). Semipreparative HPLC was performed with a Primaide 1110 solvent delivery module equipped with a 1430 photodiode array detector (Hitachi, Japan), using a YMC-Pack ODS-A column (250 × 10 mm, 5 μm; Kyoto, Japan). UV spectra were recorded on a U-2910 spectrometer (Shimadzu, Tokyo, Japan); IR spectra were obtained on an IRAffinity-1 spectrophotometer (Shimadzu). High-resolution mass spectral data were obtained on a MaXis Q-TOF mass spectrometer (Bruker, Rleigh, NC, USA). Optical rotations were recorded with an MCP-500 polarimeter (Anton Paar, Graz, Austria). NMR spectra were recorded on an Avance 500 spectrometer (Bruker) at 500 MHz for 1 H nuclei and 125 MHz for 13 C nuclei. Chemical shifts (δ) were given with reference to tetramethyl silane (TMS) or solvent residue signal. Coupling constants (J) were given in Hz.

Zhu X., Duan Y., Cui Z., Wang Z., Li Z., Zhang Y., Ju J, & Huang H. (2017). Cytotoxic rearranged angucycline glycosides from deep sea-derived Streptomyces lusitanus SCSIO LR32. The Journal of antibiotics, 70(7).

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