M. tuberculosis strains H37Rv and H37Ra were obtained from the American Type Tissue Culture (Manassas, VA). Stocks were propagated in Middlebrook 7H9 medium (Difco/Becton Dickinson, Sparks, MD) made up in low-endotoxin water (Sigma, St. Louis, MO) supplemented with albumin-dextrose-catalase supplement (Becton Dickinson) and 0.05% Tween 80 (Difco). Aliquots were stored at –80°C, thawed and propagated in Middlebrook 7H9 medium to log phase before use.
Albumin dextrose catalase supplement
Manufactured by BD
Sourced in United States
Albumin-dextrose-catalase supplement is a laboratory reagent. It is a mixture of albumin, dextrose, and catalase. The core function is to provide a source of these components for use in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using albumin dextrose catalase supplement
1
Propagation of M. tuberculosis Strains
2
Cultivation and Cryopreservation of Mycobacterial Strains
Check if the same lab product or an alternative is used in the 5 most similar protocols
M. tuberculosis H37Rv, M. celatum ATCC 51130 (strain 1908), M. fortuitum ATCC 6841, and a clinical isolate of M. abscessus (previously identified by rRNA 16S sequencing) were cultured in Dubos medium with 10% of albumin-dextrose-catalase supplement (bovine albumin fraction V 50 g/L, dextrose 20 g/L, and catalase 0.04 g/L) (Becton Dickinson, Franklin Lakes, NJ, USA) and incubated at 37°C. The bacteria were then pelleted by centrifugation and resuspended in RPMI 1640 medium (Gibco, Life Technologies, Grand Island, NY, USA) [17 ]. Viability was evaluated by counting the colony-forming units (CFUs), and the bacterial suspension was aliquoted and frozen at −70°C.
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M. tuberculosis H37Rv, M. celatum ATCC 51130 (strain 1908), M. fortuitum ATCC 6841, and a clinical isolate of M. abscessus (previously identified by rRNA 16S sequencing) were cultured in Dubos medium with 10% of albumin-dextrose-catalase supplement (bovine albumin fraction V 50 g/L, dextrose 20 g/L, and catalase 0.04 g/L) (Becton Dickinson, Franklin Lakes, NJ, USA) and incubated at 37°C. The bacteria were then pelleted by centrifugation and resuspended in RPMI 1640 medium (Gibco, Life Technologies, Grand Island, NY, USA) [17 ]. Viability was evaluated by counting the colony-forming units (CFUs), and the bacterial suspension was aliquoted and frozen at −70°C.
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