Anti cttn

Anti-CTTN (Proteintech, Whuhan, China, 11381-1-AP), HRP-conjugated goat anti-mouse IgG (CST, Boston, MA, USA, 7076), HRP-conjugated goat anti-rabbit IgG (CST, 7074), anti-GFP (Proteintech, 50430-2-AP), anti-α-tubulin (Proteintech, 11224-1-AP), anti-FLAG (Sigma, St. Louis, MO, USA, F3165), anti-HA (Proteintech, 51064-2-AP), anti-UCHL1 (CST, 13179S), anti-Ub (Santa Cruz, Dallas, TX, USA, sc-8017), Goat-anti-mouse Alexa Fluor ®488 IgG (Life Technologies, Carlsbad, CA, USA, A-10684), Goat-anti-rabbit, and Alexa Fluor ®594 IgG (Life Technologies, A-11079), MG132 (Sigma, 474787), DAC (Sigma, 189825), CHX (Sigma, 5087390001), and puromycin (Thermo, Waltham, MA, USA, A1113802) were purchased from the indicated manufacturers.

Total protein was extracted using RIPA lysis buffer containing protease and phosphatase inhibitor cocktail, EDTA-free, 100X (APT006 or APT008, AntiProtech Inc.) Western blot analysis was performed using standard procedures as previously described [18 (link)]. The antibodies used were as follows: anti-NEK9 (cat. no. ab138488, Abcam), anti-ROBO1 (cat. no. 20219-1-AP, ProteinTech Group, Inc.), anti-Flag (cat. no. MA5558, AntiProtech Inc.), anti-STAT3 (cat. no. 12640, Cell Signaling Technology, Inc.), anti-p-STAT3 (cat. no. 9145, Cell Signaling Technology, Inc), anti-mouse IgG H&L (Alexa Fluor 488) (cat. no. ab150113, Abcam), anti-TRIM28 (cat. no. 15202-1-AP, ProteinTech Group, Inc.), anti-CTTN (cat. no. sc-55579, Santa Cruz Biotechnology, Inc.) anti-pCTTN (cat. no. 4569, Cell Signaling Technology, Inc.), anti-p100 (cat. no. 4882, Cell Signaling Technology, Inc.), anti-HA (cat. no. 51064-2-AP, ProteinTech Group, Inc.), anti-p-Ser (cat. no. sc-81514, Santa Cruz Biotechnology, Inc.) and anti-β-actin (cat. no. A2228, clone AC-74, Millipore Sigma).