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Telomere repeat specific peptide nucleic acid probes

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Telomere-repeat specific peptide nucleic acid (PNA) probes are laboratory tools designed to target and detect telomeric DNA sequences. They are synthetic DNA analogues that can hybridize to complementary nucleic acid strands. These probes provide a reliable and specific method for the analysis of telomere length and structure in various research applications.

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Lab products found in correlation

Colcemid, by Roche (2 mentions) Axio imager d2 microscope, by Zeiss (2 mentions) Plan apo 1.4na, by Zeiss (2 mentions)

2 protocols using telomere repeat specific peptide nucleic acid probes

1

Chromosomal Aberrations Analysis via Telomere-FISH

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Metaphase spreads were carried out as described previously69 . Briefly, cells were irradiated with 4 J/m2 or mock-treated 48 or 72 hours before preparing metaphase spreads (final confluence of 50-80%). Cells were arrested at metaphase by incubating with colcemid (N-methyl-N-deacetyl-colchicine, Roche, 10295892001) for the last 14 hours before harvesting the cells. Collected cells were treated with hypotonic solution (KCl 0.075 M) for 30 minutes at 37°C and fixed with methanol:acetic acid 3:1. Telomere-FISH was further carried out to study chromosomal aberrations. Metaphases were hybridized with telomere-repeat specific peptide nucleic acid (PNA) probes (Applied Biosystems) as described to label telomeres70 (link). A minimum 60 metaphase images were obtained using Carl Zeiss Axio Imager D2 microscope using 63x Plan Apo 1.4 NA oil immersion objective and analyzed with ImageJ software for chromosomal aberrations.
Geijer M.E., Zhou D., Selvam K., Steurer B., Mukherjee C., Evers B., Cugusi S., van Toorn M., van der Woude M., Janssens R.C., Kok Y.P., Gong W., Raams A., Lo C.S., Lebbink J.H., Geverts B., Plummer D.A., Bezstarosti K., Theil A.F., Mitter R., Houtsmuller A.B., Vermeulen W., Demmers J.A., Li S., van Vugt M.A., Lans H., Bernards R., Svejstrup J.Q., Chaudhuri A.R., Wyrick J.J, & Marteijn J.A. (2021). Elongation factor ELOF1 drives transcription-coupled repair and prevents genome instability. Nature cell biology, 23(6), 608-619.
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2

Chromosomal Aberrations Analysis via Telomere-FISH

Check if the same lab product or an alternative is used in the 5 most similar protocols
Metaphase spreads were carried out as described previously69 . Briefly, cells were irradiated with 4 J/m2 or mock-treated 48 or 72 hours before preparing metaphase spreads (final confluence of 50-80%). Cells were arrested at metaphase by incubating with colcemid (N-methyl-N-deacetyl-colchicine, Roche, 10295892001) for the last 14 hours before harvesting the cells. Collected cells were treated with hypotonic solution (KCl 0.075 M) for 30 minutes at 37°C and fixed with methanol:acetic acid 3:1. Telomere-FISH was further carried out to study chromosomal aberrations. Metaphases were hybridized with telomere-repeat specific peptide nucleic acid (PNA) probes (Applied Biosystems) as described to label telomeres70 (link). A minimum 60 metaphase images were obtained using Carl Zeiss Axio Imager D2 microscope using 63x Plan Apo 1.4 NA oil immersion objective and analyzed with ImageJ software for chromosomal aberrations.
Geijer M.E., Zhou D., Selvam K., Steurer B., Mukherjee C., Evers B., Cugusi S., van Toorn M., van der Woude M., Janssens R.C., Kok Y.P., Gong W., Raams A., Lo C.S., Lebbink J.H., Geverts B., Plummer D.A., Bezstarosti K., Theil A.F., Mitter R., Houtsmuller A.B., Vermeulen W., Demmers J.A., Li S., van Vugt M.A., Lans H., Bernards R., Svejstrup J.Q., Chaudhuri A.R., Wyrick J.J, & Marteijn J.A. (2021). Elongation factor ELOF1 drives transcription-coupled repair and prevents genome instability. Nature cell biology, 23(6), 608-619.
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