Bl306a

Manufactured by Biosharp

The BL306A is a high-performance centrifuge designed for a variety of laboratory applications. It features a compact and durable construction, with a maximum speed of 6,000 RPM and a maximum relative centrifugal force (RCF) of 4,000 x g. The unit is equipped with an imbalance detection system and automatic shut-off for safety.

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Lab products found in correlation

04 001 1acs, by Sartorius (2 mentions) Egf 50482 mnch lc13ja1403, by Sino Biological (2 mentions) Bfgf 50177 m08h lc12se1702, by Sino Biological (2 mentions) Penicillin streptomycin solution, by Beyotime (1 mentions) Zq500 s, by Shanghai Zhong Qiao Xin Zhou Biotechnology (1 mentions) Csp006, by Shanghai Zhong Qiao Xin Zhou Biotechnology (1 mentions)

3 protocols using bl306a

BMSC Culture Protocol with Growth Factors

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BMSC complete medium consisted of α-MEM basic medium (BL306A, Biosharp) plus 10% fetal bovine serum (04-001-1ACS, Biological Industries), 1% penicillin–streptomycin solution (C0222, Beyotime), 20 ng/mL EGF (50482-MNCH LC13JA1403, Sino Biological), and 20 ng/mL bFGF (50177-M08H LC12SE1702, Sino Biological).

Liu S., Fan M., Xu J.X., Yang L.J., Qi C.C., Xia Q.R, & Ge J.F. (2022). Exosomes derived from bone-marrow mesenchymal stem cells alleviate cognitive decline in AD-like mice by improving BDNF-related neuropathology. Journal of Neuroinflammation, 19, 35.

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Isolation and Culture of Murine Pulmonary Fibroblasts

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The lungs were separated from 9‐week‐old mice anesthetized and perfused as previously described, minced, and digested to generate pulmonary fibroblasts, which were cultured in 90% α‐MEM (BL306A; Biosharp) with 10% FBS (ZQ500‐S; Zhong Qiao Xin Zhou Biotechnology), 100 U mL⁻¹ penicillin, and 0.1 mg mL⁻¹ streptomycin (CSP006; Zhong Qiao Xin Zhou Biotechnology).

Zhou J., Chen H., Wang Q., Chen S., Wang R., Wang Z., Yang C., Chen A., Zhao J., Zhou Z., Mao Z., Zuo G., Miao D, & Jin J. (2022). Sirt1 overexpression improves senescence‐associated pulmonary fibrosis induced by vitamin D deficiency through downregulating IL‐11 transcription. Aging Cell, 21(8), e13680.

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Culturing Bone Marrow-Derived Stem Cells

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Cell culture medium BMSCs complete medium consists of α-MEM basic medium (BL306A, biosharp) plus 10% fetal bovine serum (04-001-1ACS, Biological Industries), 1% penicillin-streptomycin solution (C0222, Beyotime), 20ng/mL EGF (50482-MNCH LC13JA1403, Sino Biological), and 20ng/mL bFGF (50177-M08H LC12SE1702, Sino Biological).

Liu S., Fan M., Xu J., Ge J., Xia Q., & Ge J. (2021). Protective Effect of Exosomes Derived from Bone Marrow Mesenchymal Stem Cells Against AD-Like Behaviors in Mice: Involvement with Regulating Glial Activation and its Associated Neuroinflammation and BDNF-Related Neuropathological Changes in the Hippocampus.

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