For surface marker staining, cells were stained in PBS supplemented with 1% FBS for 30 min on ice with the following antibodies (PerCP‐conjugated anti‐CD3, BV421‐conjugated anti‐CD4, PE‐conjugated anti‐CD8, BV570‐conjugated anti‐CD45, BV421‐conjugated anti‐F4/80, BV650‐conjugated anti‐CD86, APC‐conjugated anti‐CD206, PE‐Cy7‐conjugated anti‐CD25, FITC‐conjugated anti‐γδ TCR, APC‐Cy7‐conjugated anti‐CD11b, PE‐conjugated anti‐Ly‐6G, FITC‐conjugated anti‐CD49b, PE‐conjugated anti‐CD19, PE‐conjugated anti‐ST2 antibodies, and APC‐conjugated anti‐NKp46: BD Biosciences, San Jose, CA, USA). After fixation and permeabilization, staining with an APC‐conjugated anti‐FOXP3 antibody (eBioscience) was performed according to the manufacturer's instructions. For IL‐33 staining of primary mouse hepatocytes, cells were stained using a biotinylated anti‐IL‐33 monoclonal antibody (Enzo Life Biosciences, Raamsdonksveer, The Netherlands) and PE‐Cy7‐labeled streptavidin (BD Pharmingen, San Diego, CA, USA). All the flow cytometric data were analyzed and plotted using FlowJo software (TreeStar, Ashland, OR, USA).
Apc conjugated anti nkp46
Manufactured by BD
Sourced in United States
APC-conjugated anti-NKp46 is a flow cytometry reagent that binds to the NKp46 receptor expressed on the surface of natural killer (NK) cells. It can be used to identify and quantify NK cell populations in biological samples.
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Lab products found in correlation
Percp conjugated anti cd3, by BD (2 mentions)
Pe cy7 conjugated anti cd25, by BD (1 mentions)
Pe conjugated anti ly6g, by BD (1 mentions)
Pe conjugated anti cd8, by BD (1 mentions)
Bv421 conjugated anti cd4, by BD (1 mentions)
Apc conjugated anti cd206, by BD (1 mentions)
Fitc conjugated anti cd49b, by BD (1 mentions)
Pe conjugated anti cd19, by BD (1 mentions)
Pe cy7 labeled streptavidin, by BD (1 mentions)
Apc conjugated anti foxp3 antibody, by Thermo Fisher Scientific (1 mentions)
Apc cy7 conjugated anti cd11b, by BD (1 mentions)
Fitc conjugated anti nkg2d, by Thermo Fisher Scientific (1 mentions)
Fitc conjugated anti cd158a, by BD (1 mentions)
Pe conjugated anti cd158b, by BD (1 mentions)
Pe cy7 conjugated anti cd56, by BD (1 mentions)
Pe conjugated anti granzyme b, by BD (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
K562 cells, by National Collection of Authenticated Cell Cultures (1 mentions)
2 protocols using apc conjugated anti nkp46
1
Comprehensive Immune Cell Phenotyping by Flow Cytometry
2
NK Cell Cytotoxicity Assay with K562 Cells
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K562 cells (China Center for Type Culture Collection, Wuhan, China) were used as target cells in the NK cytotoxicity assay. K562 cells were cultured in RPMI 1640 (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Hyclone, Logan, UT, USA). 3,3′-Dioctadecyloxacarbocyanine perchlorate (DIO) (1911717; Invitrogen, San Diego, CA, USA) was dissolved in dimethyl sulfoxide (Sigma, St. Louis, MO, USA) to a concentration of 3 mM. Propidium iodide (PI; MKCB0899V; Sigma) was used to detect apoptosis.
Flow cytometry to identify NK cells and subpopulations expressing certain markers and effector molecules was performed using the following fluorophore-conjugated monoclonal mouse anti-human antibodies: PerCP-conjugated anti-CD3 (347344; BD Pharmingen, San Jose, CA, USA), PE-cy7-conjugated anti-CD56 (557747; BD Pharmingen), PE-conjugated anti-NKp30 (12–3379; eBioscience ThermoFisher, Waltham, MA, USA), APC-conjugated anti-NKp46 (17–3359; BD Pharmingen), FITC-conjugated anti-NKG2D (11–5878; eBioscience), FITC-conjugated anti-CD158a (556062; BD Pharmingen), PE-conjugated anti-CD158b (559785; BD Pharmingen), PE-conjugated anti-granzyme B (561142; BD Pharmingen), Alexa Fluor 647-conjugated anti-perforin (563576; BD Pharmingen), and Alexa Fluor 488-conjugated anti-granulysin (558254; BD Pharmingen).
Flow cytometry to identify NK cells and subpopulations expressing certain markers and effector molecules was performed using the following fluorophore-conjugated monoclonal mouse anti-human antibodies: PerCP-conjugated anti-CD3 (347344; BD Pharmingen, San Jose, CA, USA), PE-cy7-conjugated anti-CD56 (557747; BD Pharmingen), PE-conjugated anti-NKp30 (12–3379; eBioscience ThermoFisher, Waltham, MA, USA), APC-conjugated anti-NKp46 (17–3359; BD Pharmingen), FITC-conjugated anti-NKG2D (11–5878; eBioscience), FITC-conjugated anti-CD158a (556062; BD Pharmingen), PE-conjugated anti-CD158b (559785; BD Pharmingen), PE-conjugated anti-granzyme B (561142; BD Pharmingen), Alexa Fluor 647-conjugated anti-perforin (563576; BD Pharmingen), and Alexa Fluor 488-conjugated anti-granulysin (558254; BD Pharmingen).
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