Tin 2 2 ethylhexanoate sn

4-Cyano-4-[(dodecylsulfanylthiocarbonyl)sulfanyl]pentanol (CDSP, containing 15 wt % ethyl acetate as residual solvent), tin(II) 2-ethylhexanoate (Sn(Oct)₂, 95%), ε-caprolactone (ε-CL, 99%), concanavalin A (ConA, Type VI, lyophilized powder) lectin, mucin from porcine stomach (type II) and 2,2′-azoisobutyronitrile (AIBN, 98%) were purchased from Sigma-Aldrich Chemical Co (St. Louis, MO, USA), Doxorubicin (DOX) hydrochloric acid salt (DOX·HCl, 99%) was purchased from LC Laboratories (Woburn, MA, USA). All other regents were purchased from Sigma-Aldrich or Merck Chem. Co. (Darmstadt, Germany), and were used without further purification. Pentafluorophenyl acrylate (PFA) and 2-aminoethyl-α-d-mannopyranoside were synthesized according to the methods reported in the literature [18 (link),19 (link)].

The following chemicals were utilized in experiments: amikacin disulfate (AMI; Interquim); L-lactic acid (LA; 80% water solution, Merck Milipore); Tin(II) 2-ethylhexanoate (Sn(Oct)2; ~95%; Sigma-Aldrich, Schnelldorf, Germany,); solvents—chloroform, acetone, methanol (MeOH) and ethanol (all analytical-grade, Sigma-Aldrich, Schnelldorf, Germany); chloroform (HPLC-grade; Chromspec, Prague, Czech Republic); Fumed silica (SIAM; mean particle size 30 nm; mean pore size 6 nm; Sigma-Aldrich, Schnelldorf, Germany); Tetraethyl orthosilicate (TEOS; 98%; Sigma-Aldrich, Schnelldorf, Germany); ammonia (25%), dimethylformamide (Merck Millipore, Darmstadt, Germany) and ultrapure water—utilized as precursor materials for the synthesis of mesoporous silica nanoparticles; and diiodomethane (Sigma-Aldrich, Schnelldorf, Germany).
Antibacterial testing was performed on the following bacterial strains (Tomas Bata University in Zlin, Czech Republic): S. aureus CCM 4516; E. coli CCM 4517; Enterococcus faecalis CCM 3956; K. pneumoniae CCM 4415; and P. aeruginosa CM 1961.