Agilent 1260 infinity 2 multi detector gpc sec system

The molecular weight characteristics (weight average and number average molecular weights, M_w and M_n, respectively) and the polydispersity index (PDI)) of arabinogalactans modified with carboxylic acids were determined by gel permeation chromatography on an Agilent 1260 Infinity II Multi-Detector GPC/SEC System (Agilent Technologies, Santa Clara, CA, USA) with triple detection: a refractometer (RI), a viscometer (VS), and light scattering (LS). The separation was performed on two combined PL Aquagel-OH Mixed-M columns, using 0.1 M NaNO₃ and 250 ppm NaN₃ in water as a mobile phase. The columns were calibrated using polyethylene glycol standards (Agilent, Santa Clara, CA, USA). The eluent flow rate was 1 mL/min and the sample volume was 100 μL. Before the analysis, the samples were dissolved in the mobile phase (5 mg/mL) and filtered through a 0.45 µm Agilent PES membrane filter (Merck Millipore, Burlington, MA, USA). The data collection and processing was performed using the Agilent GPC/SEC MDS software version 2.2.
The molecular weights, M_w and M_n, and the PDI value were determined from the calibration curve obtained using polyethylene glycol polydisperse standards.

The average molecular weight M_w, number average molecular weight M_n, and the polydispersity index (PDI) of the azo lignins were determined by gel permeation chromatography (GPC) using an Agilent 1260 Infinity II Multi-Detector GPC/SEC System (Agilent Technologies, Santa Clara, CA, USA) with triple detection: refractometer (RI), viscometer (VS), and light scattering (LS). The separation was performed on two combined PL Aquagel-OH Mixed-M columns using the mixture 0.2 M NaNO₃ + 0.01 M NaHPO₄ as the mobile phase for the aqueous solutions and tetrahydrofuran (THF) as the mobile phase for the organic solutions. The columns were calibrated using polyethylene glycol (PEG) and polystyrene (PS) polydisperse standards (Agilent, Santa Clara, CA, USA). The eluent flow rate was 1 mL/min and the injected sample volume was 100 µL. Before the analysis, the water-soluble samples were dissolved in water (1.5 mg/mL) and the remaining samples, due to their insolubility in water, were dissolved in THF (1.5 mg/mL) and filtered through a 0.45 µm Millipore PTFE membrane filter. The data collection and processing were performed using the Agilent GPC/SEC MDS software version 2.2.