CD4 (ab133616) and p24 (ab9071) antibodies were purchased from Abcam. APC anti-human tetherin (348410) and APC mouse IgG1 isotype control were from Biolegend. HLA-C antibody (MABF233) was from Millipore. SNAT1 antibody (sc-67080) was from Santa Cruz. GAPDH antibody (14C10) was from cell signaling. Mouse and rabbit HRP-conjugated antibodies were from Abcam. Alexa Fluor 488 conjugated anti-mouse IgG2b antibody (A-21141) and mouse IgG2b isotype control (MA5-14447) were from ThermoFisher Scientific. Polyclonal antibody against a conserved region of HIV-1 Vpu (both subtype A and B) was raised in mouse by conjugating KLH (keyhole limpet hemocyanin) to a synthetic peptide (ERAEDSGNESDG) corresponding to amino acids 48–59 of Vpu.
Apc mouse igg1 isotype control
Manufactured by BioLegend
Sourced in Germany
The APC mouse IgG1 isotype control is a laboratory reagent used as a reference control in flow cytometry experiments. It is a mouse monoclonal antibody conjugated to the fluorescent dye allophycocyanin (APC). This control is used to determine the level of non-specific binding and establish gating strategies when analyzing cell populations.
Automatically generated - may contain errors
Lab products found in correlation
P24 ab9071, by Abcam (2 mentions)
Apc anti human tetherin 348410, by BioLegend (2 mentions)
Hla c antibody mabf233, by Merck Group (2 mentions)
Snat1 antibody sc 67080, by Santa Cruz Biotechnology (2 mentions)
Alexa fluor 488 conjugated anti mouse igg2b antibody a 21141, by Thermo Fisher Scientific (2 mentions)
Mouse igg2b isotype control ma5 14447, by Thermo Fisher Scientific (2 mentions)
Gapdh antibody 14c10, by Cell Signaling Technology (2 mentions)
Mouse and rabbit hrp conjugated antibodies, by Abcam (2 mentions)
Aria fusion cytometer, by BD (1 mentions)
Apc conjugated ov6 antibody, by R&D Systems (1 mentions)
Bv421 conjugated anti cd34 antibody clone 581, by BD (1 mentions)
Facsaria 3, by BD (1 mentions)
4 protocols using apc mouse igg1 isotype control
1
Comprehensive Antibody Panel for HIV-1 Analysis
2
Sorting of HuH6 Cells by CD34 and OV-6
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The HuH6 cells were simultaneously stained with a BV421-conjugated anti-CD34 antibody (clone 581, BD Horizon, Franklin Lakes, NJ, USA) and an APC-conjugated OV-6 antibody (R&D Systems, Biotechne, Wiesbaden, Germany). The CD34 and OV-6 double-negative cells were sorted in a BD Aria Fusion cytometer. The sorting gates were established using cells stained with isotype controls (Brilliant Violet 421 mouse IgG1 isotype control and APC mouse IgG1 isotype control, Biolegend, Koblenz, Germany). After the sorting, the collected CD34−OV-6− and CD34+OV-6+ cells were re-analyzed for CD34 expression and OV-6 binding using the cytometer.
3
Comprehensive Antibody Panel for HIV-1 Analysis
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CD4 (ab133616) and p24 (ab9071) antibodies were purchased from Abcam. APC anti-human tetherin (348410) and APC mouse IgG1 isotype control were from Biolegend. HLA-C antibody (MABF233) was from Millipore. SNAT1 antibody (sc-67080) was from Santa Cruz. GAPDH antibody (14C10) was from cell signaling. Mouse and rabbit HRP-conjugated antibodies were from Abcam. Alexa Fluor 488 conjugated anti-mouse IgG2b antibody (A-21141) and mouse IgG2b isotype control (MA5-14447) were from ThermoFisher Scientific. Polyclonal antibody against a conserved region of HIV-1 Vpu (both subtype A and B) was raised in mouse by conjugating KLH (keyhole limpet hemocyanin) to a synthetic peptide (ERAEDSGNESDG) corresponding to amino acids 48–59 of Vpu.
4
Multiparameter Flow Cytometry Analysis
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Cells were counted, and stained in cold MACS buffer (PBS, 5% BSA, 2 mM EDTA). Cell staining was done using the following antibodies: anti-human APC-CD74 (Clone: 5–329, Miltenyi Biotec), anti-human PE-HLA-DR, DP, DQ (clone: REA332, Miltenyi Biotec), anti-human FITC-CD14 (Clone: TÜK4, Miltenyi Biotec), anti-human APC-B7H4 (clone: MIH43, Biolegend), APC-Mouse IgG1 isotype control (clone: MOPC-21, Biolegend) according to manufacturer’s instructions. Cells were analyzed and sorted on a BD FACSAriaIII.
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