HepG2 cells were cultured at 37 °C with 5% CO2 in DMEM medium containing 10% fetal bovine serum and 50,000 units of antibiotics (penicillin and streptomycin). For VCP inhibition experiments, cells were treated with 15 μM NMS-873 (MedChem Express) or treated with vehicle. For knockdown experiments, VCP siRNA (s14765; Life Technologies), RHBDL4 siRNA (s38699; Life Technologies), gp78 siRNA (s1324; Life Technologies), HRD1 siRNA (s39019; Life Technologies), or negative control siRNA (sc-37007; Santa Cruz Biotechnology) was transfected at the final concentration of 100 nM using Opti-MEM (Gibco) and Lipofectamine RNAiMAX (Invitrogen). After 48 h of treatment or transfection, cells were harvested for Western blot analysis or quantitative real-time PCR analysis.
Vcp sirna
Manufactured by Thermo Fisher Scientific
The VCP siRNA is a laboratory tool designed for gene silencing experiments. It is a short, double-stranded RNA molecule that targets the VCP (Valosin-Containing Protein) gene, allowing for the study of its cellular functions.
Automatically generated - may contain errors
Lab products found in correlation
Opti mem, by Thermo Fisher Scientific (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
Mouse monoclonal antibody against actin, by Merck Group (1 mentions)
Hp dna transfection reagent, by Roche (1 mentions)
X tremegene sirna, by Roche (1 mentions)
Dynabeads protein a, by Thermo Fisher Scientific (1 mentions)
Superscript vilo cdna synthesis kit, by Thermo Fisher Scientific (1 mentions)
2 protocols using vcp sirna
1
VCP Inhibition and Knockdown in HepG2 Cells
2
AMFR Protein Expression and Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primers, Dynabeads protein A, Superscript VILO cDNA synthesis kit, VCP siRNA, and SVIP siRNA were purchased from Life Technologies (Carlsbad, CA), and X-tremeGENE siRNA and, HP DNA transfection reagent were purchased from Roche Applied Science (Indianapolis, IN). The rabbit polyclonal antibodies against AMFR and ubiquitin and anti-rabbit IgG (conformation specific) were purchased from Cell Signaling (Danvers, MA). The rabbit polyclonal antibody against N-terminus AMFR was purchased from LifeSpan BioScience (Seattle, WA). Polyclonal anti-rabbit antibody against SVIP and mouse monoclonal antibody against actin were purchased from Sigma-Aldrich (St. Louis, MO) Polyclonal anti-rabbit antibodies against p97/VCP and IP lysis buffer were purchased from Thermo Fisher scientific (Waltham, MA). Polyclonal anti-rabbit antibody against AAT was purchased from Dako (Carpentaria, CA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!